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Histogenetic capacity of rat and mouse embryonic shields cultivatedin vitro

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Summary

The organ culture technique was used for the study of early cytodifferentiation in explanted rat and mouse embryonic shields. After 15 daysin vitro the main tissues were differentiated in explants. The full differentiation depended on the presence of homologous serum in the culture medium. 95% oxygen in the atmosphere was either deleterious or without measurable effect if introduced from the beginning or toward the end of the cultivation period, respectively. Some chemically defined media supported the development for only a limited time span during the initial period of cultivation.

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Authors and Affiliations

  1. Department of Biology and Department of Histology and Embryology, Faculty of Medicine, University of Zagreb, Yugoslavia

    Nikola Škreb & Anton Švajger

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  1. Nikola Škreb
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  2. Anton Švajger
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This investigation was partly supported by NIH PL 480 Research Agreement No. 02-138-1.

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Škreb, N., Švajger, A. Histogenetic capacity of rat and mouse embryonic shields cultivatedin vitro . W. Roux' Archiv f. Entwicklungsmechanik 173, 228–234 (1973). https://doi.org/10.1007/BF00573116

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  • DOI: https://doi.org/10.1007/BF00573116

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