Summary
A procedure is presented by which four previously described morphogenetic substances can be purified from hydra: an activator and an inhibitor of head formation and an activator and an inhibitor of foot formation. We show that all four substances act specifically. At low concentrations, the head factors only influence head and not foot formation, and the foot factors only influence foot and not head formation.
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Berking, S.: Bud formation inhydra: Inhibition by an endogenous morphogen. Wilhelm Roux's Archives181, 215–225 (1977)
Berking, S., Gierer, A.: Analysis of early stages of budding in hydra by means of an endogenous inhibitor. Wilhelm Roux's Archives182, 117–129 (1977)
Bode, H., Berking, S., David, C.N., Gierer, A., Schaller, H.C., Trenkner, E.: Quantitative analysis of cell types during growth and morphogenesis in hydra. Wihelm Roux's Archives171, 269–285 (1975)
Grimmelikhuijzen, C.J.P., Schaller, H.C.: Isolation of a substance activating foot formation in hydra. Cell Differ.6, 297–305 (1977)
Lenhoff, H.M., Brown, R.D.: Mass culture in hydra: an improved method and its application to other aquatic invertebrates. Lab. Animals4, 139–154 (1970)
MacWilliams, H.K., Kafatos, F.C., Bossert, W.H.: The feedback inhibition of basal disc regeneration in hydra has a continuously variable intensity. Dev. Biol.23, 380–398 (1970)
Schaller, H.C.: Isolation and characterization of a low-molecular-weight substance activating head and bud formation in hydra. J. Embryol. Exp. Morphol.29, 27–38 (1973)
Schaller, H.C.: Action of the head activator on the determination of interstitial cells in hydra. Cell. Differ.5, 13–20 (1976)
Schaller, H.C., Schmidt, T., Flick, K., Grimmelikhuijzen, C.J.P.: Analysis of morphogenetic mutants of hydra. I. The Aberrant. Wilhelm Roux's Archives183 193–206 (1977)
Schmidt, T., Schaller, H.C.: Evidence for a foot-inhibiting substance in hydra. Cell Differ.5, 151–159 (1976)
Shostak, S.: The complexity of hydra: homeostasis, morphogenesis, controls and intergation. Rev. Biol.49, 287–310 (1974)
Webster, G.: Morphogenesis and pattern formation in hydroids. Biol. Rev.46, 1–46 (1971)
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Schaller, H.C., Schmidt, T. & Grimmelikhuijzen, C.J.P. Separation and specificity of action of four morphogens from hydra. Wilhelm Roux' Archiv 186, 139–149 (1979). https://doi.org/10.1007/BF00848175
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DOI: https://doi.org/10.1007/BF00848175