Summary
A substance was isolated from crude extracts of hydra that inhibits foot regeneration. This substance, the foot inhibitor, has a molecular weight of ≦500 daltons. It is a hydrophilic molecule, slightly basic in character and it has no peptide bonds. The pruified substance acts specifically and at concentrations lower than 10−7 M. At this low concentration only foot and not head regeneration is inhibited. Hydra are sensitive to purified foot inhibitor between the second and eight hour after initiation of foot regeneration by cutting. In normal animals the foot inhibitor is most likely produced by nerve cells. A substance with similar biological and physico-chemical properties is found in other coelenterates.
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References
Berking S (1977) Localization and purification of a substance which inhibits bud formation in hydra. Wilhelm Roux's Archives 181:215–225
Berking S (1979) Analysis of head and foot information in hydra by means of an endogenous inhibitor. Wilhelm Roux's Archives 186:189–210
Bode H, Berking S, Gierer A, Schaller H, Trenkner E (1973) Quantitative analysis of cell types during growth and morphogenesis in hydra. Wilhelm Roux's Archives 171:269–285
David CN (1973) Quantitative method for maceration of hydra tissue. Wilhelm Roux's Archives 171:259–268
Diamant B, Redlich D v, Glick D (1967) Comparison of the phenolreagent and brosulfalein-binding methods for determination of protein and its measurement in mast cells. Anal Biochem 21:135–146
Diehl F, Burnett AL (1964) The role of interstitial cells in maintenance of hydra. I. Specific destruction of interstitial cells in normal, asexual, non-budding animals. J Exp Zool 155:253–260
Epp L, Tardent P (1978) The distribution of nerve cells in Hydra attenuata Pall. Wilhelm Roux's Archives 185:185–193
Grimmelikhuijzen CJP (1979) Properties of the foot activator from hydra. Cell Differ 8:267–273
Grimmelikhuijzen CJP and Schaller HC (1977) Isolation of a substance activating foot formation in hydra. Cell Differ 6:297–305
Lenhoff HM, Brown RD (1970) Mass culture in hydra: an improved method and its application to other aquatic invertebrates. Lab Anim 4:139–154
MacWilliams HK, Kafatos FC (1968) Inhibition by the basal disc of basal disc differentiation in hydra. Science 159:1246–1247
MacWilliams HK, Kafatos FC (1974) The basal inhibition in hydra may be mediated by a diffusing substance. Am Zool 14:633–645
Schaller H (1973) Isolation and characterization of a low-molecular-weight substance activating head and bud formation in hydra. J Embryol Exp Morphol 29:27–38
Schaller H, Gierer A (1973) Distribution of head activating substance in hydra and its localisation in membranous particles in nerve cells J Embryol Exp Morphol 29:39–52
Schaller HC, Schmidt T, Grimmelikhuijzen CJP (1979) Separation and specificity of action of four morphogens from hydra. Wilhelm Roux's Archives 186:139–149
Schmidt T, Schaller HC (1976) Evidence for a foot-inhibiting substance in hydra. Cell Differ 5:151–159
Webster G (1971) Morphogenesis and pattern formation in hydroids. Biol Rev 46:1–46
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Schmidt, T., Schaller, H.C. Properties of the foot inhibitor from hydra. Wilhelm Roux' Archiv 188, 133–139 (1980). https://doi.org/10.1007/BF00848805
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DOI: https://doi.org/10.1007/BF00848805