Summary
A mixed type of pituitary adenoma is described consisting of heavily and sparsely granulated cells. It produces GH and prolactin (PRL) and has been examined by immunocytochemistry. The superimposition immunocytochemical procedure reveals that single cells contain both GH and PRL. Furthermore, electron immunocytochemistry using adjacent sections reveals that single secretory granules contain both GH and PRL simultaneously.
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References
Baskin DG, Erlandsen SL, Parsons JA (1979) Immunocytochemistry with osmium-fixed tissue I. Light microscopic localization of growth hormone and prolactin with the unlabeled antibody-enzyme method. J Histochem Cytochem 27:867–972
Baskin DG, Erlandsen SL, Parson JA (1980) Functional classification of cell types in the growth hormone- and prolactin-secreting rat MtTW15 mammosomatotropic tumor with ultrastructural immunocytochemistry. Am J Anat 158:455–461
Corenblum B, Sirek AMP, Horvath E, Ezrin C (1976) Human mixed somatotrophic and lactotrophic pituitary adenoma. J Clin Endocrinol Metab 42:857–863
Fukaya T, Kageyama N, Kuwayama A, Takanohashi M, Okada C, Yoshida J, Osamura Y (1980) Morphofunctional study of pituitary adenoma with acromegaly by immunoperoxidase technique and electron microscopy. Cancer 45:1598–1603
Guyda H, Robert F, Colle E, Hardy J (1973) Histologic ultrastructural, and hormonal characterization of a pituitary tumor secreting both hGH and prolactin. J Clin Endocrinol Metab 36:531–547
Horvath E, Kovacs K (1976) Ultrastructural classification of pituitary adenoma. Can J Neurol Sci 3:9–21
Ishikawa H, Nogami H, Kamio M, Suzuki T (1983) Immunocytochemical studies of mixed human somatotrophic and lactotrophic pituitary adenoma. Jikeikai Med J (in press)
Kovacs K, Horvath E, Pritzker KPH, Schwarts ML (1977) Pituitary growth hormone cell adenoma with cytoplasmic tubular aggregates in the capillary endothelium. Acta Neuropathol (Berl) 37:77–79
Landolt AM (1978) Progress in pituitary adenoma biology: Results of research and clinical applications, In: Krayenbuhl H (ed) Advances and technical standards in neurosurgery. Springer Vienna and New York, p 3
Lewis PD, Van Noorden S (1974) “Nonfunctioning” pituitary tumors. Arch Pathol (Chicago) 97:178–182
Millonig G (1962) Further observation on a phosphate buffer for osmium solutions in fixation. Electron microscopy. Fifth international congress for electron microscopy, Academic Press 2:8
Mollenhauer HH (1964) Plastic embedding mixtures for use in electron microscopy. Stain Technol 39:111–114
Robert F (1973) Etude macroscopique, histologique et ultrastructurale. Neurochirurgie (Paris) 19, suppl 2:117–162
Robert F, Hardy J (1975) Prolactin-secreting adenoma. A light and electron microscopical study. Arch Pathol (Chicago) 99:625–633
Yoshida J, Kageyama N, Seo H, Kanzaki M (1975) Growth hormone and prolactin secretion of pituitary adenoma. Neurol Med-Chir (Tokyo) 15:13–21
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Ishikawa, H., Nogami, H., Kamio, M. et al. Single secretory granules contain both GH and prolactin in pituitary mixed type of adenoma. Vichows Archiv A Pathol Anat 399, 221–226 (1983). https://doi.org/10.1007/BF00619581
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DOI: https://doi.org/10.1007/BF00619581