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Pigmented esthesioneuroblastoma showing dual differentiation following transplantation in nude mice

An immunohistochemical, electron microscopical, and cytogenetic analysis

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Summary

Esthesioneuroblastoma (ESTH) is a neuroepithelial-cell-derived neoplasm of the olfactory mucosa composed of homogeneous small round cells which contain neurosecretory granules. Melanin has been detected in such tumours only occasionally. Here we describe a new case of ESTH with divergent differentiation. The primary neoplasm was found in a 67 year-old female, involving the left nasal and maxillary sinus; she died of cerebral metastasis ten months after diagnosis. Histologically only small round cells were seen, with S-100 and NSE positivity. Electron microscopy revealed neurosecretory granules and filaments, as well as the occasional presence of melanosomes. A nude mice xenograft line has been established, and is presently in its ninth transfer. Two cell types are present: small round-to-spindle shaped cells with neural features, and large epithelial-like ones. Both immunohistochemistry and electron microscopy confirm this dual differentiation, with the presence of membrane-bound dense-core neural secretion, as well as melanosomes of neuroectodermal origin. Additionally, an in vitro cell line has been established. Cytogenetic analysis confirmed the presence of both malignant human melanoma patterns; non-random abnormalities in chromosomes 1 and 6, extra copies of chromosome 7. Duplication of the long arm of chromosome 14, as seen in olfactory neuroblastoma, is also seen.

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Authors and Affiliations

  1. Department of Pathology Hospital Clinico, University of Valencia Medical School, Av Blasco Ibañiez 17, 46010, Valencia, Spain

    A. Llombart-Bosch, C. Carda, A. Peydro-Olaya, R. Noguera, J. Boix & A. Pellin

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  1. A. Llombart-Bosch
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  2. C. Carda
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  3. A. Peydro-Olaya
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  4. R. Noguera
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  5. J. Boix
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  6. A. Pellin
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Additional information

This work has been supported, in part, with a grant (83/058) from the US-Spain Joint Committee for Scientific and Technological Cooperation (Madrid). This paper was presented, in abbreviated form, at the 72nd Tagung of the Deutsche Gesellschaft für Pathologie, Hannover (24–28 May, 1988)

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Llombart-Bosch, A., Carda, C., Peydro-Olaya, A. et al. Pigmented esthesioneuroblastoma showing dual differentiation following transplantation in nude mice. Vichows Archiv A Pathol Anat 414, 199–208 (1989). https://doi.org/10.1007/BF00822023

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  • DOI: https://doi.org/10.1007/BF00822023

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