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Large cell anaplastic lymphoma: Evaluation of immunophenotype on paraffin and frozen sections in comparison with ultrastructural features

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Summary

Eleven cases of large cell anaplastic lymphoma (T typen=5, B typen=4, 0 typen=2) were investigated using electron microscopy and immunophenotyping on formalin-fixed paraffin sections and frozen sections of fresh tissue, to determine whether morphological criteria exist for the discrimination of T, B, and 0 phenotypes. Tumour cell lineage could not be established from ultrastructural features. On paraffin material monoclonal B-cell markers Ki-B5 and L-26 served as reliable tools for recognizing the B phenotype of large cell anaplastic lymphomas (previously determined on fresh material), whereas monoclonal antibodies MT1 (CD43) and UCHL1 (CD45RO) were of limited value in lineage determination.

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Authors and Affiliations

  1. Department of Pathology, University of Kiel, Michaelisstrasse 11, W-2300, Kiel 1, Federal Republic of Germany

    M. -L. Hansmann & A. Bohm

  2. Department of Pathology, Technical University of Munich, School of Medicine, Ismaninger Strasse 22, W-8000, Munich 80, Federal Republic of Germany

    C. Fellbaum

Authors
  1. M. -L. Hansmann
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  2. C. Fellbaum
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  3. A. Bohm
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Hansmann, M.L., Fellbaum, C. & Bohm, A. Large cell anaplastic lymphoma: Evaluation of immunophenotype on paraffin and frozen sections in comparison with ultrastructural features. Vichows Archiv A Pathol Anat 418, 427–433 (1991). https://doi.org/10.1007/BF01605929

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  • DOI: https://doi.org/10.1007/BF01605929

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