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Expression of MDR1/p-glycoprotein and multidrug resistance-associated protein in childhood solid tumours

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Abstract

We evaluated the expression of MDR1/p-glycoprotein in paediatric tumours using reverse transcriptase polymerase chain reaction (RT-PCR), RNA dot blot analysis, and immunohistochemistry on formalin fixed paraffin-embedded material with JSB-1 and C-219 monoclonal antibodies, and compared these three techniques. The expression of multidrug resistance-associated protein (MRP) gene was examined by RT-PCR assay. We studied MDR1/p-glycoprotein and MRP expression in 13 samples from 10 neuroblastoma patients, 11 samples from 10 nephroblastoma patients, 2 rhabdomyosarcomas, 1 adrenocortical carcinoma and 10 benign tumours or tumour-like lesions. Eleven of 13 neuroblastomas, 7 of 11 nephroblastomas, 2 rhabdomyosarcomas, 1 adrenocortical carcinoma, and 7 of 10 benign tumours or tumour-like lesions showed MDR1 PCR products. By RNA dot blot analysis, MDR1 transcripts were detectable in 11 of 34 specimens. Immunohistochemically, we detected positive reaction products for JSB-1 in 26 of 36 samples. There was a significant correlation between the immunoreactivity for JSB-1 and the expression of MDR1 mRNA expression by RTPCR (P=0.0001). However, the presence of p-glycoprotein immunostaining does not correlate with the MDR1 expression shown by RT-PCR in every case. As for MRP mRNA expression, 9 of 13 neuroblastomas and 10 of 11 nephroblastomas revealed PCR products.

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Authors and Affiliations

  1. Department of Pathology, Otto-von-Guericke University Magdeburg, Leipziger Strasse 44, D-39120, Magdeburg, Germany

    Y. Oda, I. Röse, K. Radig & A. Roessner

  2. Department of Pediatric Surgery, Otto-von-Guericke University, Magdeburg, Germany

    W. Wagemann

  3. Department of Pediatric Oncology, Otto-von-Guericke University, Magdeburg, Germany

    U. Mittler

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  1. Y. Oda
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  2. I. Röse
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Oda, Y., Röse, I., Radig, K. et al. Expression of MDR1/p-glycoprotein and multidrug resistance-associated protein in childhood solid tumours. Virchows Archiv 430, 99–105 (1997). https://doi.org/10.1007/BF01008030

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  • DOI: https://doi.org/10.1007/BF01008030

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