Summary
In 195 female Wistar rats the influence of adrenalectomy, total body X-irradiation and dexamethasone treatment on the amount of CRF-granules in the external zone of the median eminence and “classical” neurosecretory material (NSM) in the internal zone of the median and in the posterior lobe of the hypophysis has been studied.
In the normal rat only very few CRF-granules occur. 15 days after adrenalectomy increased amounts of CRF-granules but normal amounts of “classical” NSM are found. X-irradiation leads to a slight diminishment of “classical” NSM in normal rats. In adrenalectomized animals X-irradiation causes no changes in the amount of “classical” NSM but a marked decrease in the amount of CRF-granules. Application of dexamethasone from the 15th to 18th day after adrenalectomy enhances the augmentation of CRF-granules in irradiated and non-irradiated rats but does not influence the amount of “classical” NSM.
The findings show that no correlation exists between the quantitative changes of CRF-granules and “classical” NSM under the experimental conditions used. Therefore it is to be assumed that the release of the two substances is regulated by different control mechanisms and that the substances have a different functional significance. The observations do not exclude the possibility that CRF-granules and “classical” NSM are biochemically identical.
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References
Anderson, E.: Adrenocorticotrophin-releasing hormone in peripheral blood; increase during stress. Science 152, 379–380 (1966)
Arko, H., Kivalo, E., Rinne, U. K.: Hypothalamo-neurohypophysial neurosecretion after the extirpation of various endocrine glands. Acta endocr. (Kbh.) 42, 293–299 (1963)
Bach, J. H., Hennes, K. H.: Einfluß von Hydrocortison auf die Menge “Gomori-positiver” Substanzen in der Zona externa infundibuli bilateral adrenalektomierter Ratten. J. Neural Transmission 33, 11–22 (1972)
Bargmann, W.: Über die neurosekretorische Verknüpfung von Hypothalamus und Neurohypophyse. Z. Zellforsch. 34, 610–634 (1949)
Bargmann, W.: Das Zwischenhirn-Hypophysensystem. Berlin-Göttingen-Heidelberg: Springer 1954
Bargmann, W.: neurosecretion. Int. Rev. Cytol. 19, 183–201 (1966)
Bock, R.: Morphometrische Untersuchungen zum histologischen Nachweis des Corticotropin-releasing factor im Infundibulum der Ratte. Z. Anat. Entwickl.-Gesch. 137, 1–29 (1972)
Bock, R., Brinkmann, H., Feldmann, M.: Influence of corticoids on the amount of CRF granules in the median eminence of adrenalectomized rats. In: Neurosecretion—the final neuroendocrine pathway (ed. F. Knowles and L. Vollrath), p. 297. Berlin-Heidelberg-New York: Springer 1974
Bock, R., Brinkmann, H., Marckwort, W.: Färberische Beobachtungen zur Frage nach dem primären Bildungsort von Neurosekret im supraoptico-hypophysären System. Z. Zellforsch. 87, 534–544 (1968)
Bock, R., Forstner, R. v.: Beiträge zur funktionellen Morphologie der Neurohypophyse. II. Vergleichsuntersuchung histologischer Veränderungen im Infundibulum der Ratte nach beidseitiger Adrenalektomie und nach Hypophysektomie. Z. Zellforsch. 94, 434–440 (1969)
Bock, R., aus der Mühlen, K.: Beiträge zur funktionellen Morphologie der Neurohypophyse. I. Über eine “gomoripositive” Substanz in der Zona externa infundibuli beidseitig adrenalektomierter weißer Mäuse. Z. Zellforsch. 92, 130–148 (1968)
Bock, R., Salland, Th., Schwabedal, P., Watkins, W.: Histochemical and immunohistochemical properties of the CRF-granules and other “Gomori-positive” substances of the rat. In preparation (1975)
Brinkmann, H., Bock, R.: Quantitative Veränderungen “Gomori-positiver” Substanzen in Infundibulum und Hypophysenhinterlappen der Ratte nach Adrenalektomie und Kochsalz- oder Durstbelastung. J. neuro-visceral. Relat. 32, 48–64 (1970)
Brinkmann, H., Bock, R.: Influence of various corticoids on the augmentation of “Gomori-positive” granules in the median eminence of the rat following adrenalectomy. Naunyn-Schmiedebergs Arch. Pharmak. 280, 49–62 (1973)
Brodish, A., Redgate, E. S. (editors): Brain-pituitary-adrenal interrelationships. Basel-München-Paris-London-New York-Sydney: S. Karger 1973
Burler, A., Marchetti, J., Duheille, J.: Immunohistochemistry of vasopressin: Study of the hypothalymo-hypophysial system of normal, dehydrated and hypophysectomized rats. In: Neurosecretion—the final neuroendocrine pathway (ed. F. Knowles and L. Vollrath), p. 24–30. Berlin-Heidelberg-New York: Springer 1974
Chan, L. T., de Wied, D., Saffran, M.: Comparison of assays for corticotrophin releasing activity. Endocrinology, 84, 967–972 (1969)
Chowers, I., Conforti, N., Feldmann, S.: Effects of corticosteroids on hypothalamic corticotropin releasing factor and pituitary ACTH content. Neuroendocrinology 2, 193–199 (1967)
Doepfner, W.: The influence of neurohypophysial polypeptides on adenohypophysial function. In: Handbuch der experimentellen Pharmakologie (hrsg. O. Eichler, A. Farah, H. Herken, A. D. Welch), vol. XXIII. Neurohypophysial hormones and similar polypeptides (ed. B. Berde), p. 625–654. Berlin-Heidelberg-New York: Springer 1968
Donald, R. A.: Dexamethason suppression of the plasma corticosterone response to stress in the rat. J. Physiol. (Lond.) 182, 603–611 (1966)
Eichner, D.: Über den morphologischen Ausdruck funktioneller Beziehungen zwischen Nebennierenrinde und neurosekretorischem Zwischenhirnsystem der Ratte. Z. Zellforsch. 38, 488–508 (1953)
Elde, R. P.: Methods for the localization of vasopressin-containing neurons in the hypothalamus and pars nervosa by immunoenzyme histochemistry. Anat. Rec. 175, 313–314 (1973)
Evans, J. J., Watkins, W.: Localization of neurophysin in the neurosecretory elements of the hypothalamus and neurohypophysis of the normal and osmotically stimulated guinea pig as demonstrated by immunofluorescence histochemical techniques. Z. Zellforsch. 145, 39–55 (1973)
Feldmann, M.: Wirkung einer Substitutionsbehandlung mit DOCA oder Dexamethason auf die Vermehrung “Gomori-positiver” Granula im Infundibulum der Ratte nach Adrenalektomie. Einfluß von Zeitpunkt des Behandlungsbeginns und Behandlungsdauer. Inaug.-Dissert. Med. Fak. Bonn 1975
Gaunt, R., Chart, J. J.: Mineralocorticoid action of adrenocortical hormones. In: Handbuch der experimentellen Pharmakologie (shrsg. O. Eichler und A. Farah), Bd. XIV, Teil 1. The adrenocortical hormones. Their origin, chemistry, physiology, and pharmakology (Subed. H. W. Deane), p. 514–569. Berlin-Göttingen-Heidelberg: Springer 1962
Goldman, L., Winget, C., Hollingshead, G. W., Levine, S.: Postweaning development of negative feedback in the pituitary-adrenal system of the rat. Neuroendocrinology 12, 199–211 (1973)
Gomori, G.: Observations with differential stains on human islets of Langerhans. Amer. J. Path. 17, 395–406 (1941)
Groot, J. de, Hartfield, J. E.: Quantitative changes in rat pituitary neurosecretory material in altered adrenocortical function. Acta neuroveg. (Wien) 22, 177–183 (1960/61)
Guillemin, R.: Hypothalamic factors releasing pituitary hormones. Recent Progr. Hormone Res. 20, 89–130 (1964)
Hiroshige, T., Sato, T., Abe, K.: Dynamic changes in the hypothalamic content of the corticotropin-releasing factor following noxious stimuli: delayed response in early neonates in comparison with biphasic response in adult rats. Endocrinology 89, 1287–1294 (1971)
Hodges, J. R., Jones, M. T.: Changes in the pituitary corticotrophic function in the adrenalectomized rat. J. Physiol. (Lond.) 173, 190–200 (1964)
Hodges, J. R., Vernikos, J.: Circulating corticotrophin in normal and adrenalectomized rats after stress. Acta endocr. (Kbh.) 30, 188–196 (1959)
Hodges, J. R., Vernikos, J.: The effects of hydrocortison on the level of corticotrophin in the blood and pituitary glands of adrenalectomized and of stressed adrenalectomized rats. J. Physiol. (Lond.) 150, 683–693 (1960)
Hope, D. B., Pickup, J. C.: Neurophysins. In: Handbook of physiology. Section 7: Endocrinology (ed. R. O. Greep and E. B. Astwood), vol. IV: The pituitary gland and its neuroendocrine control, part I (ed. E. Knobil ahd W. H. Wawyer), p. 173–189. Washington, USA: American Physiological Society 1974
Kloet, E. R. de, Vies, J. van der, Wied, D. de: The site of the suppressive action of dexamethasone on pituitary-adrenal activity. Endocrinology 94, 61–73 (1974)
Kohn, H. I.: Effect of immaturity, hypophysectomy and adrenalectomy upon changes in blood plasma of rat during acute radiation syndrome. Amer. J. Physiol. 165, 43–56 (1961)
Kratzsch, E., Künkel, H. A., Wander, H. H.: Das Verhalten des neurosekretorischen Zwischenhirnsystems der Ratte nach Ganzkörperbestrahlung. Z. Zellforsch. 56, 683–692 (1962)
Malandra, B., Corbetta, S.: La sostanza gomoripositiva della neuroipofisi del ratto dopo surrenalektomia e trattamento con corticoidi surrenali e sale. Z. Zellforsch. 39, 318–327 (1953)
McCann, S. M., Porter, J. C.: Hypothalamic pituitary stimulating and inhibiting hormones. Phys. Rev. 49, 240–284 (1969)
Mey, J. de, Vandesande, F., Dierickx, K.: Identification of neurophysin producing cells. II. Identification of the neurophysin I and the neurophysin II producing neurons in the bovine hypothalamus. Cell Tiss. Res. 153, 531–543 (1974)
Mills, E., Wang, S. C.: Liberation of antidiuretic hormone: pharmacological blockade of ascending pathways. Amer. J. Physiol. 207, 1405–1410 (1964)
Ochonskaya, I. A., Polenov, A. L., Selivanova, G. V.: Histospectrophotometry of the neurosecretory substance in the posterior lobe of the hypophysis. Histochemie 7, 327–329 (1966)
Pelletier, G., Leclerc, R., Labrie, F., Puviani, R.: Electrone microscope immunohistochemical localization of neurophysin in the rat hypothalamus and pituitary. Molecular and Cellular Endocrinology 1, 157–166 (1974)
Pickford, M.: Neurohypophysis—antidiuretic (vasopressor) and oxytocic hormones. In: The hypothalamus (ed. W. Haymaker, E. Anderson and W. J. H. Nauta), p. 463–505. Spring-field (Illinois, USA): Charles C. Thomas 1969
Pietrzik, K., Schwabedal, P., Hesse, Ch., Bock, R.: Influence of pantothenic acid deficiency on the amount of CRF-granules in the rat median eminence. Anat. Embryol. 146, 43–55 (1974)
Riegle, G. D., Hess, G. D.: Chronic and acute dexamethasone suppression of stress activation of the adrenal cortex in young and aged rats. Neuroendocrinology 9, 175–187
Rodeck, H., Braukmann, R.: Über die Wirkung von Nikotinsäure auf das neurosekretorische System. Z. Zellforsch. 75, 83–95 (1966a)
Rodeck, H., Braukmann, R.: Zur Frage der Wirkung von Morphiumsulfat auf das neurosekretorische System. Z. Zellforsch. 75, 517–526 (1966b)
Rodeck, H., Breuer, H. A.: Tierexperimentelle Untersuchungen zur Frage der Wirkung des akuten Hungerns auf das neurosekretorische System. Z. Zellforsch. 69, 573–586 (1966)
Rothballer, A. B.: Changes in the rat neurohypophysis induced by painful stimuli with particular reference to neurosecretory material. Anat. Rec. 115, 21–36 (1953)
Rothballer, A. B.: The neurosecretory response to stress, anaesthesia, adrenalectomy and adrenal demedullation in the rat. Acta neuroveg. (Wien) 13, 179–191 (1956)
Russel, S. M., Dhariwal, A. P. S., McCann, S. M., Yates, F. E.: Inhibition by dexamethasone of the in vivo pituitary response to corticotropin-releasing factor (CRF). Endocrinology 85, 512–521 (1969)
Sachs, H., Fawcett, P., Takabatake, Y., Portanova, R.: Biosynthesis and release of vasopressin and neurophysin. Recent Progr. Hormone Res. 25, 447–484 (1969)
Saffran, M., Schally, A. V., Segal, M., Zimmermann, B.: Characterization of the corticotropin releasing factor of the neurohypophysis. In: Zweites Int. Symp. über Neurosekretion (ed. W. Bargmann, B. Hanström, B. u. E. Scharrer), p. 55–59. Berlin-Göttingen-Heidelberg: Springer 1958
Sayers, G., Sayers, M. A.: Regulation of the pituitary adrenocorticotrophic activity during the response of the rat to acute stress. Endocrinology 40, 265–273 (1947)
Schally, A. V., Arimura, A., Bowers, C. Y., Kastin, A. Y., Sawano, S., Redding, T. W.: Hypothalamic neurohormones regulating anterior pituitary function. Recent Progr. Hormone Res. 24, 497–581 (1968)
Schally, A. V., Bowers, C. Y.: Corticotrophin-releasing factor and other hypothalamic peptides. Metabolism 13, 1190–1205 (1964)
Schally, A. V., Guillemin, R.: Isolation and chemcal characterization of a β-CRF from pig posterior pituitary glands. Proc. Soc. exp. Biol. (N.Y.). 112, 1014–1017 (1963)
Schwabedal, P. E.: Influence of stress on the amount of “Gomori-positive” granules in the outer layer of the median eminence of bilaterally adrenalectomized rats. J. Neural Transmission 35, 217–231 (1974)
Schwabedal, P. E.: Einfluß von Stress und Dexamethason auf die Menge der CRF-Granula im Infundibulum adrenalektomierter Ratten. Verhandl. Anat. Ges. 69. Versammlung, Kiel. Anat. Anz. Erg.-Bd.,im Druck (1975)
Selye, H.: A syndrome produced by diverse nocuous agents. Nature (Lond.) 138, 32 (1936)
Selye, H.: Studies on adaptation. Endocrinology 21, 169–188 (1937)
Selye, H.: The general adaptation syndrome and the disease of adaptation. J. clin Endoor. 6, 117–230 (1946)
Selye, H.: Stress (The physiology and pathology of exposure to systemic stress). Montreal: Acta Med. Publ. 1950
Selye, H.: Einführing in die Lehre vom Adaptationssyndrom. Stuttgart: G. Thieme 1953
Sirett, N. E., Gibbs, F. P.: Dexamethasone suppression of ACTH release: effect of the interval between steroid administration and the application of stimuli known to release ACTH. Endocrinology 85, 355–359 (1969)
Stark, E., Makara, G. B., Marton, J., Palkovits, M.: ACTH release in rats after removal of the medial hypothalamus. Neuroendocrinology 13, 224–233 (1973/74)
Sydnor, K. L., Sayers, G.: Blood and pituitary ACTH in intact and adrenalectomized rats after stress. Endocrinology 55, 621–631 (1954)
Vandesande, F., Dierickx, K., Mey, J. de: Identification of the vasopressin-neurophysin II and the oxytocin-neurophysin I producing neurons in the bovine hypothalamus. Cell Tiss. Res. 156, 189–200 (1975a)
Vandesande, F., Dierickx, K., Mey, J. de: Identification of separate vasopressin-neurophysin II and oxytocin-neurophysin I containing nerve fibres in the external region of the bovine median eminence. Cell Tiss. Res. 158, 509–516 (1975b)
Vernikos-Danellis, J.: Effect of acute stress on the pituitary gland: changes in blood and pituitary ACTH concentration. Endocrinology 72, 574–581 (1963)
Vernikos-Danellis, J.: Estimation of corticotropin-releasing activity of rat hypothalamus and neurohypophysis before and after stress. Endocrinology 75, 514–520 (1964)
Vernikos-Danellis, J.: Effect of stress, adrenalectomy, hypophysectomy and hydrocortisone on the corticotropin-releasing activity of rat median eminence. Endocrinology 76, 122–126 (1965a)
Vernikos-Danellis, J.: Regulation of the synthesis and release of ACTH. Vitam. and Horm. 23, 97–152 (1965b)
Watkins, W. B.: Neurosecretion in the external and internal zone of the median eminence of the cat and dog. Cell Tiss. Res. 155, 201–210 (1974)
Watkins, W. B.: Presence of neurophysin and vasopressin in the hypothalamic magnocellular nuclei of rats homozygous and heterozygous for diabetes insipidus (Brattleboro strain) as revealed by immunoperoxidase histology. Cell Tiss. Res. 157, 101–113 (1975)
Watkins, W. B., Evans, J. J.: Demonstration of neurophysin in the hypothalamo-neurohypophysial system of the normal and dehydrated rat by the use of cross-species reactive antineurophysins. Z. Zellforsch. 131, 149–170 (1972)
Watkins, W. B., Evans, J. J.: The use of formalin fixation in an improved method for immunofluorescence histochemical investigation of neurophysin in the hypothalamus. J. Histochem. Cytochem. 22, 128–130 (1974)
Wied, D. de: The site of the blocking action of dexamethasone on stress-induced pituitary ACTH release. J. Endocr. 29, 29–37 (1964)
Winkler, C., Schorn, H.: Untersuchungen über Produktion und Stoffwechsel der Corticoide nach letaler Ganzkörperbestrahlung von Meerschweinchen. Strahlentherapie 127, 121–128 (1965)
Winkler, C., Schorn, H.: Untersuchungen über Bedeutung, Biosynthese und Metabolismus der Glukokortikoide im akuten Strahlensyndrom. In: Radioaktive Isotope in Klinik und Forschung, Bd. VII. Siebentes Int. Symp. Bad Gastein 10–13. Jan. 1966 (ed. K. Fellinger, R. Höfer), p. 294–302. München-Berlin-Wien: Urban & Schwarzenberg 1967
Zimmermann, E., Smyrl, R., Critchlow, V.: Suppression of pituitary-adrenal response to stress with physiological plasma levels of corticosterone in the female rat. Neuroendoerinology 10, 246–256 (1972)
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Supported by grants from the Landesamt für Forschung, Nordrhein-Westfalen BRD and from the Schutzkommission beim Bundesministerium des Innern
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Schwabedal, P., Bock, R. & Winkler, C. Influence of adrenalectomy, total body X-irradiation and dexamethasone on the amount of CRF-Granules and “classical” neurosecretory material in the rat neurohypophysis. Anat. Embryol. 148, 267–278 (1975). https://doi.org/10.1007/BF00319847
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DOI: https://doi.org/10.1007/BF00319847