Summary
Is the presence of germinal cells necessary for the Sertoli cells to acquire normal features? To respond to this question we have studied the development of the Sertoli cells in rats irradiated at the end of the foetal life.
In the prenatal irradiated rats, the lumen of the seminiferous tubules appears later than in the control rats. The Sertoli cells show numerous flexuous apical processes, with central microtubule bundles. These processes regress progressively after the 40th day of life when the tubular lumen appears; numerous junctional complexes differentiate with the same structure as those of control animals. There are important dilatations of the intercellular spaces. The cytoplasmic organelles show a normal development up to the 40th day of life. After this period, the rough endoplasmic reticulum and the Golgi apparatus clearly regress while important dilatations appear in the smooth endoplasmic reticulum and persist in the adult animal. From the 35th day on, the basal lamina of the seminiferous tubules is irregular and multilayered.
The differentiation of the Sertoli cells seems to be independent of the presence of germinal cells until the 40th day of life and presents several particularities; thereafter the Sertoli cells show signs of regression.
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References
Abreu I, David-Ferreira JF (1982) Fine structure of seminiferous tubules from prenatally irradiated rats. Cell Tissue Res 222:143–152
Aumüller G, Hartmann K, Giers U, Schenck B (1980) Fine structure of the Sertoli cells of the rat testis in experimental unilateral cryptorchidism. Int J Androl 3:301–311
Beaumont HM (1960) Changes in the radiosensitivity of the testis during foetal development. Int J Radiat Biol 23:247–256
Bustos-Obregon E, Holstein AF (1973) On structural patterns of the lamina propria of human seminiferous tubules. Z Zellforsch 141:413–425
Cameron DF, Snydle FE, Ross MH, Drylie DM (1980) Ultrastructural alterations in the adluminal testicular compartment in men with varicocele. Fertil Steril 33:526–533
Chemes HE, Dym M, Fawcett DW, Taradpour N, Sherins RJ (1977) Pathological observations of Sertoli cells in patients with germinal aplasia or severe germ cell depletion. Ultrastructural findings and hormone levels. Biol Reprod 17:108–123
Coffigny HG, Pasquier CF, Perrault G, Dupouy JP (1978) Study in adult rats of the effects of irradiation with 150 rads at different stages during gestation and the neonatal period: effect on the development of the genital organs. In: Late biological effects of ionizing radiation Vol II Vienna. International Atomic Energy Agency 207–220
De Jong FH, Sharpe RM (1977) Gonadotrophins, testosterone and spermatogenesis in neonatally irradiated male rats: evidence for a role of the Sertoli cell in follicle-stimulating hormone feedback. J Endocrinol 75:209–219
Dym M, Cavicchia JC (1977) Further observations on the blood testis barrier in monkeys. Biol Reprod 17:390–403
Hall E, Hupp EW (1970) Localization of irradiation damage in rat seminiferous tubules. Nature 225:85–86
Handel MA, Eppig JJ (1979) Sertoli cell differentiation in the testes of mice genetically deficient in germ cells. Biol Reprod 20:1031–1038
Hatier R, Grignon G (1980) Ultrastructural study of Sertoli cells in rat seminiferous tubules during intrauterine life and the postnatal period. Anat Embryol 160:11–27
Kerr JB, Rich KA, De Kretser DM (1979) Effects of experimental cryptorchidism on the ultrastructure and function of the Sertoli cell and peritubular tissue of the rat testis. Biol Reprod 21:823–838
Lüders G (1976) Strukturelle Veränderungen der Tubulus membran beim sogenannten Sertoli-Zell-Syndrom. Arch Dermatol Res 255:193–202
Means AR, Fakunding JL, Huckings L, Tindall DJ, Vitale R (1976) Follicle-stimulating hormone, the Sertoli cell and spermatogenesis. Recent Prog Horm Res 32:477–527
Nistal M, Paniagua R (1979) Ultrastructure of testicular biopsy from an XX male. Virchows Arch (Cell Pathol) 31:45–55
Osman DI, Plöen L, Hagenäs L (1979) On the postnatal development of the terminal segment of the seminiferous tubules in normal and germ cell depleted rat testes. Int J Androl 2:419–431
Ritzen EM, Hansson V, French FS (1981) The Sertoli cell. In: Comprehensive endocrinology. In: H Burger, D de Kretser (Ed) The testis. Raven Press New York
Schulze C, Holstein AF, Schirren C, Körner F (1976) On the morphology of the human Sertoli cells under normal conditions and in patients with impaired fertility. Andrologia 8:167–178
Takayama H (1973) An electron microscopic study of the Sertoli cell in the human testis. Acta Urol Japonica 19:47–118
Terquem A, Dadoune JP (1981) Morphological findings in varicocele. Int J Androl 4:515–531
Tindall DJ, Vitale R, Means AR (1975) Androgen binding protein as a biochemical marker of formation of the blood-testis barrier. Endocrinology 97:636–648
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Hatier, R., Grignon, G. & Touati, F. Ultrastructural study of seminiferous tubules in the rat after prenatal irradiation. Anat Embryol 165, 425–435 (1982). https://doi.org/10.1007/BF00305578
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DOI: https://doi.org/10.1007/BF00305578