Summary
In previous studies pineal “synaptic” ribbons have been shown to undergo striking numerical changes under various physiological and experimental conditions and to be regulated by β-adrenergic mechanisms. The aim of the present investigation was to study the numbers of pineal “synaptic” ribbons and spherules in Wistar rats throughout the estrous cycle and to compare them with those in males. There were no statistically significant differences in the numbers of ribbons and spherules between males and females and in the females at the different stages of the estrous cycle, indicating that the structures in question, in vivo, do not appear to be regulated by naturally occurring changes of sex steroid hormones and gonadotrophins.
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References
Arendt J, Wetterberg L, Heyden T, Sizonenko PC, Paunier L (1977) Radioimmunassay of melatonin: Human serum and cerebrospinal fluid. Horm Res 8:65–75
Bunt AH (1971) Enzymatic digestion of synaptic ribbons in amphibian retinal photoreceptors. Brain Res 25:571–577
Cardinali DP (1981) Hormone effect on the pineal gland. In: Reiter RJ (ed) The pineal gland, Vol I. CRC Press, Boca Raton, Florida, pp 243–272
Cardinali DP, Vacas MI (1978) Progesterone-induced decrease of pineal protein synthesis in rats. Possible participation in estrous-related changes of pineal function. J Neural Transm 42:193–205
Cardinali DP, Nagle CA, Rosner JM (1974) Effects of estradiol on melatonin and protein synthesis in the rat pineal organ. Horm Res 5:304–310
Cardinali DP, Nagle CA, Rosner JM (1975) Control of estrogen and androgen receptors in the rat pineal gland by catecholamine transmitter. Life Sci 16:93–106
Commentz JC, Willig RP (1981) Radioimmunological determination of diurnal melatonin plasma levels in children with Turner's syndrome using a new antiserum. Acta Endocrinol 96:suppl. 240:108–109
Davis GA (1978) Response of adenosine 3′,5′-monophosphate to norepinephrine in hypothalamus and pineal organ of female rats in proestrus or diestrus. Endocrinology 103:1048–1053
Fasano AV, Kasprow BA, Velardo JT (1977) Dehydrogenase activity of the pineal gland during the estrous cycle. Endocr Res Comm 4:257–266
Gray EG, Pease HL (1971) On understanding the organization of the retinal receptor synapses. Brain Res 35:1–15
Hewing M (1979) Synaptic ribbons during postnatal development of the pineal gland in the golden hamster (Mesocricetus auratus). Cell Tissue Res 199:473–482
Hewing M (1980) Synaptic ribbons in the pineal system of normal and light deprived golden hamsters. Anat Embryol 159:71–80
Johnson LY, Vaughan MK, Richardson BA, Petterborg LJ, Reiter RJ (1982) Variation in pineal melatonin content during the estrous cycle of the rat. Proc Soc Exp Biol Med 169:416–419
Kachi T, Matsushima S, Ito T (1973) Diurnal variations in pineal glycogen content during the estrous cycle in female mice. Arch Histol Jpn 35:153–159
Karasek M (1974) Ultrastructure of rat pineal gland in organ culture; influence of norepinephrine, dibutyryl cyclic adenosine 3′,5′-monophosphate and adenohypophysis. Endokrinologie 64:106–114
Karasek M, Vollrath L (1982) “Synaptic” ribbons and spherules of the rat pineal gland: Day/night changes in vitro? Exp Brain Res 46:205–208
Karasek M, Marek K, Kunert-Radek J (1978) Ultrastructure of rat pinealocytes in vitro: Influence of gonadotropic hormones and LH-RH. Cell Tissue Res 195:547–556
Karasek M, King TS, Richardson BA, Hurlbut EC, Hansen JT, Reiter RJ (1982a) Day-night differences in the number of pineal “synaptic” ribbons in two diurnal rodents, the chipmunk (Tamias striatus) and the ground squirrel (Spermophilus richardsonii). Cell Tissue Res 224:689–692
Karasek M, King TS, Brokaw J, Hansen JT, Petterborg LJ, Reiter RJ (1982b) Inverse correlation between “synaptic” ribbon number and the density of adrenergic nerve endings in the pineal gland of various mammals. Anat Rec (in press)
Karnovsky MJ (1965) A formaldehyde-glutaraldehyde fixative of high osmomality for use in electron microscopy. J Cell Biol 27:137A-138A
King TS, Dougherty WJ (1980) Neonatal development of circadian rhythm in “synaptic” ribbon numbers in the rat pinealocyte. Am J Anat 157:335–343
King TS, Dougherty WJ (1982a) Effect of denervation on “synaptic” ribbon populations in the rat pineal gland. J Neurocytol 11:19–28
King TS, Dougherty WJ (1982b) Age-related changes in pineal “synaptic” ribbon populations in rats exposed to continuous light or darkness. Am J Anat 163:169–179
Kurumado K, Mori W (1977) A morphological study of the circadian cycle of the pineal gland of the rat. Cell Tissue Res 182:565–568
Kurumado K, Mori M (1980) Pineal synaptic ribbons in blinded rats. Cell Tissue Res 208:229–235
Lues G (1971) Die Feinstruktur der Zirbeldrüse normaler, trächtiger und experimentell beeinflußter Meerschweinchen. Z Zellforsch 114:38–60
McNeill ME, Whitehead DS (1979) The synaptic ribbons of the guinea-pig pineal gland in sterile, pregnant and fertile but non-pregnant females and in reproductively active males. J Neural Transm 45:149–164
McNulty JA (1981) Synaptic ribbons in the pineal organ of the goldfish: Circadian rhythmicity and the effects of constant light and constant darkness. Cell Tissue Res 215:491–497
Nagle CA, Cardinali DP, Rosner JM (1973) Uptake of estradiol by the rat pineal organ. Effects of cervical sympathectomy, stage of the estrous cycle and estradiol treatment. Life Sci 13:1089–1103
Nir I Hirschmann N, Sulman FG (1972) Influence of the oestrus cycle on the nucleic acid and protein content of the rat pineal gland. Experientia 28:88–89
Ozaki Y, Wurtman R, Alonso R, Lynch HJ (1978) Melatonin secretion decreases during the proestrous stage of the rat estrous cycle. Proc Natl Acad Sci USA 75:531–534
Peters A, Palay SL, Webster H de F (1976) The fine structure of the nervous system: The neurons and supporting cells WB Saunders Company Philadelphia-London-Toronto
Pévet P (1981) Ultrastructure of the mammalian pinealocyte. In: RJ Reiter (ed) The pineal gland, Vol I, Anatomy and biochemistry. CRC Press, Boca Raton, Florida
Quay WB (1963) Circadian rhythm in rat pineal serotonin and its modification by estrous cycle and photoperiod. Gen Comp Endocrinol 3:473–479
Quay WB (1964) Circadian and estrous rhythms in pineal and brain serotonin. Progr Brain Res 8:61–63
Reiter RJ (1981) The pineal. Vol 6. Annual Research Reviews. Eden Press, Montreal
Rollag MD, O'Callaghan PL, Niswender GD (1978) Serum melatonin concentrations during different stages of the annual reproductive cycle in ewes. Biol Reprod 18:279–285
Rollag MD, Chen HJ, Ferguson BN, Reiter RJ (1979) Pineal melatonin content throughout the hamster estrous cycle. Proc Soc Biol Med 162:211–213
Romijn HJ (1975) The ultrastructure of the rabbit pineal gland after sympathectomy, parasympathectomy, continuous illumination, and continuous darkness. J Neural Transm 36:183–194
Romijn HJ, Gelsema AJ (1976) Electron microscopy of the rabbit pineal organ in vitro. Evidence of norepinephrine-stimulated secretory activity of the Golgi apparatus. Cell Tissue Res 172:365–377
Rudeen PK, Reiter RJ, Vaughan MK (1975) Pineal serotonin-N-acetyltransferase activity in four mammalian species. Neurosci Letters 1:225–229
Sachs L (1978) Angewandte Statistik. Springer-Verlag. Berlin-Heidelberg-New York
Semm P, Vollrath L (1980) Electrophysiological evidence for circadian rhythmicity in a mammalian pineal organ. J Neural Transm, 47:181–190
Shivers BD, Yochim JM (1979) Pineal serotonin N-acetyltransferase activity in the rat during the estrous cycle and in response to light. Biol Reprod 21:385–391
Theron JJ, Biagio R, Meyer AC, Boekkooi S (1979) Microfilaments, the smooth endoplasmic reticulum and synaptic ribbon fields in the pinealocytes of the baboon (Papio ursinus). Am J Anat 154:151–162
Theron JJ, Biagio R, Meyer AC (1981) Circadian changes in microtubules, synaptic ribbons and synaptic ribbons fields in the pinealocytes of the baboon (Papio ursinus). Cell Tissue Res 217:405–413
Vollrath L (1973) Synaptic ribbons of a mammalian pineal gland. Circadian changes. Z Zellforsch 145:171–183
Vollrath L (1981) The pineal organ. Hdb d mikr Anat d Mensch A Oksche, L Vollrath (eds). Vol VI/7. Springer-Verlag. Berlin-Heidelberg-New York
Vollrath L, Howe C (1976) Light and drug induced changes of epiphysial synaptic ribbons. Cell Tissue Res 165:383–390
Vollrath L, Huss H (1973) The synaptic ribbons of the guinea-pig pineal gland under normal and experimental conditions. Z Zellforsch 139:417–429
Vollrath L, Schultz RL, McMillan PJ (1982) “Synaptic” ribbons and spherules of the guineapig pineal gland: Inverse day/night rhythmicity. Am J Anat (submitted)
Wallen EP, Yochim JM (1974) Rhythmic function of pineal hydroxyindole-O-methyl transferase during the estrous cycle: an analysis. Biol Reprod 10:461–466
Weiss B, Crayton J (1970) Ovarian regulation of the norepinephrine-sensitive adenyl cyclase system of rat pineal gland. Fed Proc 29:615
Wolfe DE (1965) The epiphyseal cell: An electron microscopic study of its intercellular relationships and intracellular morphology in the pineal body of the albino rat. Progr Brain Res 10:332–386
Wurtman RJ, Axelrod J, Snyder SH, Chu EW (1965) Changes of the enzymatic synthesis of melatonin in the pineal during the estrous cycle. Endocrinology 76:798–800
Wurtman RJ, Axelrod J, Kelly DE (1968) The pineal. Academic Press, New York-London
Zweens J (1963) Influence of the oestrous cycle and ovariectomy on the phospholipid content of the pineal gland in the rat. Nature (Lond) 197:1114–1115
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Supported by grant Vo 135/7 from the Deutsche Forschungsgemeinschaft within the Schwerpunktprogramm Neuroendokrinologie
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Kosaras, B., Welker, H.A. & Vollrath, L. Pineal “synaptic” ribbons and spherules during the estrous cycle in rats. Anat Embryol 166, 219–227 (1983). https://doi.org/10.1007/BF00305084
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DOI: https://doi.org/10.1007/BF00305084