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The central projections of primary afferent neurons of greater splanchnic and intercostal nerves in the rat

A horseradish peroxidase study

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Summary

The central projections of primary afferent fibers of the greater splanchnic nerve of the rat were investigated using the transganglionic horseradish peroxidase transport technique. In addition, the corresponding spinal ganglion cells and the preganglionic sympathetic neurons were demonstrated. For comparing visceral and somatic afferents, intercostal nerve afferents were labelled by the same technique.

Splanchnic afferent dorsal root ganglion cells were found at segments T3 to T13 ipsilaterally, with the greatest density at T8 to T12. Labelled cells represented about 10%–15% of all neurons in the ganglia at maximal projection levels. They were randomly distributed within individual ganglia. The great majority were medium to small sized and round to slightly oval in shape.

In the spinal cord, labelled visceral afferent axons were found maximally at T8 to T11, but could be detected in decreasing density up to T1 and down to L1. They were distributed over Lissauer's tract and the dorsal funiculus to a medial and lateral collateral pathway (MCP and LCP, respectively). The MCP, somewhat more prominent than the LCP, was destined primarily to clustered presumptive terminal fields in medial lamina I and outermost lamina IIa. Only a few axons continued further to laminae V and X. Splanchnic afferent axons, most likely derived from the MCP, formed a longitudinal bundle ventral to the central canal. The LCP consisted of more or less well-defined axon bundles emanating from the lateral Lissauer's tract and curving round the lateral edge of the dorsal horn and through the dorsolateral funiculus. Presumptive terminal sites of LCP axons are the lateral laminae I and IIa, the nucleus of the dorsolateral funiculus and the dorsal part of lamina V. A few LCP axons were seen in the vicinity of lateral dendrites of preganglionic sympathetic axons. Visceroafferent terminals were absent from laminae IIb–IV and VII. The possible consequences of the MCP/LCP duality for the central connections of splanchnic afferents are discussed. Some splanchnic afferents ascended to the gracile and cuneate nuclei, and rarely to the spinal trigeminal nucleus.

These results fit into the general concept of visceroafferent terminal organization that has emerged during the last few years. Differences to other reports in the detailed arrangement of fibers and terminals are discussed.

Somatoafferent cell bodies represented the vast majority of neurons in the respective spinal ganglia. Cell sizes encompassed the whole range from very small to very large without a clear predominance of one particular size class. Cell shapes of somatic neurons were more variable than those of visceral afferent neurons. Somatic afferent fibers and presumptive terminals in the spinal cord are distributed ipsilaterally to dorsal horn laminae I–V, most heavily II–IV, to the nucleus dorsalis Clarke, to the ventral horn, and also sparsely to the dorsal horn contralaterally.

Labelled preganglionic sympathetic neurons were found in segments T3–T13. The vast majority was located in the intermediolateral nucleus. Fewer neurons occurred in the intercalated nucleus, and occasionally a neuron was labelled in the dorsal grey commissure.

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Authors and Affiliations

  1. Institute of Anatomy, University of Zürich, Zürich, Switzerland

    W. L. Neuhuber, P. A. Sandoz & T. Fryscak

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  1. W. L. Neuhuber
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  2. P. A. Sandoz
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  3. T. Fryscak
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Dedicated to Prof. Dr. W. Zenker on occasion of his 60th birthday

Parts of this study have been presented in abstract form at the 8th ENA meeting in Den Haag, September 1984

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Neuhuber, W.L., Sandoz, P.A. & Fryscak, T. The central projections of primary afferent neurons of greater splanchnic and intercostal nerves in the rat. Anat Embryol 174, 123–144 (1986). https://doi.org/10.1007/BF00318344

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  • DOI: https://doi.org/10.1007/BF00318344

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