Summary
The dorsal pancreatic primordia of 12.5-day-old rat embryos transplanted into the third ventricle of adult female rats were immunohistochemically examined 10, 20 and 40 days after transplantation. On day 10, the grafts grew into an epithelial sacculus (S) with a thick subepithelial tissue (ST). Tubular and vesicular structures with a single cuboidal epithelium were found within the wall of the S, but they underwent thereafter a regression without allowing the primordia to differentiate into the exocrine acinar tissues. In contrast with this, pancreatic hormone-containing cells existed in the ST, and were arranged like the islands of a mature animal. The tissue also has smooth muscle fibers and neurons. When the primordium was grafted along with its root connected to the duodenum, gut-like tubular structures differentiated, showing mucosa with villi and crypts, submucous mesenchymal tissue and muscle layers. The mucosa possesses epithelial cells immunoreactive for the pancreatic hormones, and the muscle layers have the myenteric plexuses. These findings seem to provide further evidence that in the rat pancreas, pancreatic-hormone-containing cells differ from the acinar cells in origin.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Alpert S, Hanahan D, Teitelman G (1988) Hybrid insulin genes reveal a developmental lineage for pancreatic endocrine cells and imply a relationship with neurons. Cell 53:295–308
Andrew A (1984) The development of the gastro-entero-pancreatic neuroendocrine system in birds. In: Falkmer S, Hakanson R, Sundler F (eds) Proc. of the 5th E.K. Fernstrom Symposium. Elsevier, Amsterdam, pp 91–111
Buchan AMJ, Gregor M, Riecken E-O (1987) Immunocytochemical characterization of glucagon-immunoreactive cells using monoclonal antibodies to pancreatic glucagon. Histochemistry 87:79–83
Cantino D (1970) An histochemical study of the nerve supply to the developing alimentary tract. Experientia 26:766–767
Chikamori-Aoyama M, Kawano H, Ootani T, Yokote R, Okamura Y, Daikoku S, Yanaihara N (1986) Ontogenesis of neurons containing proopiomelanocortin related- and proenkephalin A related-peptides in the rat hypothalamus: in vivo and transplantation studies. Biomed Res 7:233–244
Daikoku S, Kawano H, Okamura Y, Tokuzen M, Nagatsu I (1986) Ontogensis of immunoreactive tyrosine hydroxylase-containing neurons in rat hypothalamus. Dev Brain Res 28:85–98
Daikoku S, Chikamori-Aoyama M, Tokuzen M, Okamura Y, Kagotani Y (1988) Development of hypothalamic neurons in intraventricular grafts: expression of specific transmitter phenotypes. Dev Biol 126:382–393
Drews U (1975) Cholinesterase in embryonic development. Prog Histochem Cytochem 7:1–52
Fontaine J, Le Lièvre C, Le Douarin NM (1977) What is the developmental fate of the neural crest cells which migrate into the pancreas in the avian embryo? Gen Comp Endocrinol 33:394–404
Fritsch HAR, Van Noorden S, Pearse AGE (1976) Cytochemical and immunofluorescence investigations on insulin-like producing cells in the intestine ofMytilus edulis L. (Bivalvia). Cell Tissue Res 165:365–369
Gonet AE, Renold AE (1965) Homografting of fetal pancreas. Diabetologia 1:91–96
Håkanson R, Lundquist I (1971) Occurrence of insulin in rat duodenum and its depletion with alloxan. Experientia 27:1220–1221
Halban PA, Powers SL, George KL, Bonner-Weir S (1987) Spontaneous reassociation of dispersed adult rat pancreatic islet cells into aggregates with three-dimensional architecture typical of native islets. Diabetes 36:783–790
Hashimoto T, Kawano H, Daikoku S, Shima K, Taniguchi H, Baba S (1988) Transient coappearance of glucagon and insulin in the progenitor cells of the rat pancreatic islets. Anat Embryol 178:489–497
Hegre OD, Leonard RJ, Rusin JD, Lazarow A (1976) Transplantation of the fetal rat pancreas: Quantitative morphological analysis of islet tissue growth. Anat Rec 185:209–222
Hisano S, Chikamori-Aoyama M, Katoh S, Maegawa M, Daikoku S (1987) Immunohistochemical evidence of serotoninergic regulation of vasoactive intestinal polypeptide (VIP) in the rat suprachiasmatic nucleus. Histochemistry 86:573–578
Hisano S, Chikamori-Aoyama M, Katoh S, Kagotani Y, Daikoku S, Chihara K (1988) Suprachiasmatic nucleus neurons immunoreactive for vasoactive intestinal polypeptide have synaptic contacts with axons immunoreactive for neuropeptide Y: an immunoelectron microscopic study in the rat. Neurosci Lett 88:145–150
Ito Y, Sohma S, Hirano H (1984) Light- and electron-microscopic studies on acetylcholinesterase activity in Auerbach’s plexus of the developing rat colon. Histochemistry 81:209–212
Kawano H, Daikoku S (1988) Somatostatin-containing neuron systems in the rat hypothalamus. Retrograde tracing and immunohistochemical studies. J Comp Neurol 271:293–299
Kawano H, Daikoku S, Saito S (1982) Immunohistochemical studies of intrahypothalamic somatostatin-containing neurons in rat. Brain Res 242:227–232
Kühl C, Jensen SL, Nielsen OV (1976) Porcine gastric insulin. Endocrinology 99:1667–1670
Le Douarin M (1978) The embryological origins of the endocrine cells associated with the digestive tract: experimental analysis based on the use of a stable cell marking technique. In: Bloom SR (ed) Gut Hormones. Churchill, London, pp 49–56
McEvoy RC, Leung PE (1983) Transplantation of fetal rat islets into the cerebral ventricles of alloxan-diabetic rats. Amelioration of diabetes by syngeneic but not allogeneic islets. Diabetes 32:852–857
Millard PR, Path MRC, Garvey JFW, Jeffery EL, Morris PJ (1980) The grafted fetal rat pancreas. Features of development and rejection. Am J Pathol 100:209–224
Pearse AGE (1977) The diffuse neuroendocrine system and the APUD concept: related “endocrine” peptides in brain, intestine, pituitary, placenta, and anuran cutaneous glands. Med Biol 55:115–125
Pearse AGE (1982) Islet cell precursors are neurons. Nature 295:96–97
Popiela H, Tomita T, Hegre O, Moore W (1986) Islet neoformation in tissue culture. Mol Cell Endocrinol 48:11–20
Rothman TP, Gershon MD (1982) Phenotypic expression in the developing murine enteric nervous system. J Neurosci 2:381–393
Rothman TP, Nilaver G, Gershon MD (1984) Colonization of the developing murine enteric nervous system and subsequent phenotypic expression by the precursors of peptidergic neurons. J Comp Neurol 225:13–23
Smith J, Fauquet M, Ziller C, Le Douarin NM (1979) Acetylcholine synthesis by mesencephalic neural crest cells in the process of migration in vivo. Nature 282:853–855
Teitelman G, Lee JK (1987) Cell lineage analysis of pancreatic islet cell development: glucagon and insulin cells arise from catecholaminergic precursors present in the pancreatic duct. Dev Biol 121:454–466
Teitelman G, Joh TH, Reis DJ (1978) Transient expression of a noradrenergic phenotype in cells of the rat embryonic gut. Brain Res 158:229–234
Tsuruo Y, Kawano H, Nishiyama T, Hisano S, Daikoku S (1983) Substance P-like immunoreactive neurons in the tuberoinfundibular area of rat hypothalamus. Light and electron microscopy. Brain Res 289:1–9
Yoshinari M, Daikoku S (1982) Ontogenetic appearance of immunoreactive endocrine cells in rat pancreatic islets. Anat Embryol 165:63–70
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Daikoku, S., Hashimoto, T. & Yokote, R. Development of the dorsal pancreatic primordium transplanted into the third ventricle of rats. Anat Embryol 181, 441–452 (1990). https://doi.org/10.1007/BF02433791
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02433791