Abstract
The efferent neurons of the lateral line system of the euteleost Aplocheilus lineatus and the osteoglossomorph Pantodon buchholzi, both surface feeding fish, were examined by neuronal tract tracing. Besides horseradish peroxidase, fluorescent dextrans were used as tracers to allow simultaneus visualization of projections from different lateral line branches. Labeled efferent neurons were found in nuclei situated in the medulla ventral of ventricle IV. This position resembles the octavolateralis efferent nucleus of previous studies. The number of labeled cells in the efferent nucleus is low in both species. Most neurons were found ipsilaterally to the application site, some along the midline and only very few contralaterally. The size of efferent cells differs distinctly between Aplocheilus, possessing small cellbodies (length 16.5 μm), and Pantodon, which has very large efferent cells (length 47.0 μm). Efferent axon bundles course rostrally in both species, leaving the brain at the level of the anterior lateral line nerve. Only Aplocheilus has in addition lateral axon bundles leaving the brain at the level of the posterior lateral line nerve. After application of one fluorescent tracer to the lateral ramus and a different fluorescent tracer to the superficial ophtalmic ramus in a given animal, double-labeling of efferent cells hardly ever occurs. If the neuromasts I and IV of the dorsal skull of Pantodon are applied with one fluorescent tracer each, ∼10% of centrally labeled cells are double-labeled. Considering the results of double-labeling, the concept of a differential innervation of lateral line branches is supported and discussed.
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Adams JC (1981) Heavy metal intensification of DAB-based HRP reaction product. J Histochem Cytochem 29: 775
Adams JC, Mroz EA, Sewell WF (1987) A possible neurotransmitter role for CGRP in a hair-cell sensory organ. Brain Res 419: 347–351
Bell CC (1981) Central distribution of octavolateral afferents and efferents in a teleost (Mormyridae). J Comp Neurol 195: 391–414
Bleckmann H, Tittel G, Blübaum-Gronau E (1989) The lateral line system of surface-feeding fish: anatomy, physiology and behavior. In: Coombs S, Görner P, Münz H (eds) The mechanosensory lateral line — neurobiology and evolution. Springer, Berlin Heidelberg New York, pp 501–526
Bleckmann H, Niemann U, Fritzsch B (1991) Peripheral and central aspects of the acoustic and lateral line system of a bottom dwelling catfish, Ancistrus sp. J Comp Neurol 314: 452–466
Blübaum-Gronau E, Münz H (1987) Topologische Repräsentation primärer Afferenzen einzelner Seitenlinienabschnitte beim Schmetterlingsfisch Pantodon buchholzi. Verh Dtsch Zool Ges 80: 268–269
Claas B, Münz H (1980) Bonyfish lateral line efferent neurons identified by retrograde axonal transport of horseradish peroxidase (HRP). Brain Res 193: 249–253
Claas B, Fritzsch B, Münz H (1981) Common efferents to lateral line and labyrinthine hair-cells in aquatic vertebrates. Neurosci Lett 27: 231–235
Flock Å, Russell IJ (1976) Inhibition by efferent nerve fibres: action on hair-cells and afferent synaptic transmission in the lateral line canal organ of the burbot, Lota lota. J Physiol (Lond) 257: 45–62
Fritzsch B (1988) The amphibian octavo-lateralis system and its regressive and progressive evolution. Acta Biol Hung 39: 305–322
Fritzsch B (1989) Diversity and regression in the amphibian lateral line and electrosensory system In: Coombs S, Görner P, Münz H (eds) The mechanosensory lateral line — neurobiology and evolution. Springer, Berlin Heidelberg New York, pp 99–114
Fritzsch B, Wilm C (1990) Dextran amines in neuronal tracing. Trends Neurosci 13: 14
Fritzsch B. Crapon de Caprona D (1984) The origin of centrifugal inner ear fibers of gymnophions (amphibia). A horseradish peroxidase study. Neurosci Lett 81: 131–136
Fritzsch B, Dubuc R, Ohta Y, Grillner S (1989) Efferents to the labyrinth of the river lamprey (Lampetra fluviatilis) as revealed with retrograde tracing techniques. Neurosci Lett 96: 241–246
Fritzsch B, Christensen MA, Nichols DH (1993) Fiber pathways and positional changes in efferent perikarya of 2.5- to 7-day chick embryos as revealed with DiI and dextran amines. J Neurobiol 24: 1481–1499
Gimlich RL, Braun J (1985) Improved fluorescent Compounds for tracing cell lineage. Dev Biol 109: 509–514
Glover JC, Petursdottir G, Jansen JKS (1986) Fluorescent dextranamines used as axonal tracers in the nervous system of the chicken embryo. J Neurosci Methods 43: 986–1025
Gonzalez A, Meredith GE, Roberts BL (1993) Choline acetyltransferase immunoreactive neurons innervating labyrinthine and lateral line sense organs in amphibians. J Comp Neurol 332: 258–268
Görner P (1967) Independence of afferent activity from efferent activity in the lateral line organ of Xenopus laevis Daudin. In: Lateral line detectors. Indiana University Press, pp 199–217
Highstein SM, Baker R (1986) Organization of the efferent vestibular nuclei and nerves of the Toadfish Opsanus tau. J Comp Neurol 243: 309–325
Klinke R, Schmidt CL (1970) Efferent influence on the vestibular organ during active movements of the body. Pflügers Arch 318: 325–332
Klüver H, Barrera E (1953) A method for the combined staining of cells and fibers in the nervous system. J Neuropathol Exp Neurol 12: 400–403
Kohyama H, Kishida R, Goris RC, Kusunoki T (1989) Afferent and efferent projections of the VIIIth cranial nerve in the lamprey Lampetra japonica. J Comp Neurol 280: 663–671
La Vail MM, La Vail JH (1972) Retrograde intraaxonal transport of horseradish peroxidase in retinal ganglion cells of the chick. Brain Res 85: 273–280
Meredith GE, Roberts BL (1986) Central organization of the efferent supply to the labyrinthine and lateral line receptors of the dogfish. Neuroscience 17: 225–233
Meredith GE, Roberts BL (1987) Distribution and morphological characteristics of efferent neurons innervating end organs in the ear and lateral line of the european eel. J Comp Neurol 265: 494–506
Metcalfe WK, Kimmel CB, Schabtach E (1985) Anatomy of the posterior lateral line system in young larvae of the zebrafish. J Comp Neurol 233: 377–389
Münz H, Claas B (1991) Activity of the lateral line efferents in the axolotl (Ambystoma mexicanum). J Comp Physiol [A] 169: 461–469
New JG, Singh S (1994) Central topography of anterior lateral line nerve projections in the channel catfish, Ictalurus punctatus. Brain Behav Evol 43: 34–50
Puzdrowski RL (1989) Peripheral distribution and central projections of the lateral line nerves in goldfish Carassius auratus. Brain Behav Evol 34: 110–131
Roberts BL, Meredith GE (1989) The efferent system. In: Coombs S, Görner P, Münz H (eds) The mechanosensory lateral line neurobiology and evolution. Springer, Berlin Heidelberg New York, pp 446–459
Roberts BL, Meredith GE (1992) The efferent innervation of the ear: variations on an enigma. In: Fay RA, Popper AN, Webster DB (eds) The evolutionary biology of hearing. Springer, Berlin Heidelberg New York, pp 185–210
Roberts BL, Maslam S, Los I, Jagt B van der (1994) Coexistence of calcitonin gene-related peptide and choline acetyltransferase in eel efferent neurons. Hear Res 74: 231–237
Russell IJ (1971) The role of the lateral line efferent system in Xenopus laevis. J Exp Biol 54: 621–641
Schellart NAM, Prins M, Kroese ABA (1992) The pattern of trunk lateral line afferents and efferents in the rainbow trout (Salmo gairdneri). Brain Behav Evol 39: 371–380
Schmidt RS (1965) Amphibian acoustico-lateralis efferents. J Cell Comp Physiol 65: 155–162
Schwartz E (1965) Bau und Funktion der Seitenlinie des Streifenhechtlings. Z Vergl Physiol A 50: 55–87
Schwartz E (1970) Ferntastsinnesorgane von Oberflächenfïschen. Z Morphol Tiere 67: 40–57
Song J, Northcutt RG (1991) The primary projections of the lateral-line nerves of the florida gar, Lepisosteus platyrhincus. 37: 38–63
Tittel G, Müller U (1984) Vergleichende Anatomie der Seitenlinien von Aplocheilus lineatus (Cyprinidontiformes) und von Pantodon buchholzi (Pantodontidae) — beeinflußt die Struktur die Leistungsfähigkeit? Verh Dtsch Zool Ges 77: 311
Wagner T, Schwartz E (1992) Efferent lateral-line neurons of teleosts and their projection to lateral-line segments. Proc Göttingen Neurobiol Conference 20: 251
Will U (1982) Efferent neurons of the lateral line system and the VIII cranial nerve in the brainstem of anurans. Cell Tissue Res 225: 673–685
Zottoli SJ, Horne C van (1983) Posterior lateral line afferent and efferent pathways within the central nervous system of the goldfish with special reference to the Mauthner cell. J Comp Neurol 219: 100–111
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Wagner, T., Schwartz, E. Efferent neurons of the lateral line system and their innervation of lateral line branches in a euteleost and an osteoglossomorph. Anat Embryol 194, 271–278 (1996). https://doi.org/10.1007/BF00187138
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DOI: https://doi.org/10.1007/BF00187138