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Sensitive quantitation of unlabelled SV40 cRNA by competition hybridization with3H-labelledin vitro cRNA

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Summary

A sensitive method has been developed for the quantitation of small amounts of SV40 cRNA by means of competition hybridization of3H-SV40 cRNA (transcribed in vitro with RNA polymerase fromE. coli). The rate of competition is only slightly diminished by a moderate excess of filter-bound SV40 DNA. By using a tenfold excess of SV 40 DNA and a3H-SV40 cRNA of high specific activity it is possible to obtain a competition rate of 50% with only ng amounts of unlabelled SV40 cRNA.

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Authors and Affiliations

  1. Department of Virology, Institute of Hygiene, University of Freiburg im Breisgau, Germany

    N. Mueller, E. E. Petersen & G. Brandner

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  1. N. Mueller
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  2. E. E. Petersen
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  3. G. Brandner
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Mueller, N., Petersen, E.E. & Brandner, G. Sensitive quantitation of unlabelled SV40 cRNA by competition hybridization with3H-labelledin vitro cRNA. Med Microbiol Immunol 158, 83–90 (1972). https://doi.org/10.1007/BF02120472

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  • DOI: https://doi.org/10.1007/BF02120472

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