Abstract
An indirect ELISA using whole tachyzoites ofToxoplasma gondii fixed onto the bottom of microtiter plate wells is described for detection of specific G and M anti-toxoplasma antibodies. ELISA results were compared with those of IFA and IHA tests. Similarity of antigens (cell surface) involved in ELISA and IFA permits high correlation between the two tests (r = 0.84-0.93,P < 0.001) and results in good accordance for negative sera. As ELISA offers several advantages over the classical IFA test, it may be considered an alternative method for screening of G and M anti-toxoplasma antibodies.
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Tomasi, J.P., Barka, N. & Stadtsbaeder, S. Serodiagnosis of human G and M immunoglobulins toToxoplasma gondii by ELISA using whole tachyzoites as antigens: a comparative study with the indirect haemagglutination (IHA) and immunofluorescence (IFA) tests. Med Microbiol Immunol 175, 261–269 (1986). https://doi.org/10.1007/BF02123734
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DOI: https://doi.org/10.1007/BF02123734