Abstract
Experimental autoimmune orchitis (EAO) was induced in SMA mice (H-2nondefined) by repeated injection at intervals of 30 days of syngeneic testis homogenate (TH) together with Klebsiella O3 lipopolysaccharide (KO3 LPS) as a potent adjuvant. EAO was not induced by repeated injection of TH alone or KO3 LPS alone. At 10 days after the secondary injection of TH + KO3 LPS, there was marked infiltration with neutrophils in the seminiferous tubules and in the interstitium of the testis accompanied by destruction of the architecture of the seminiferous tubules and hypospermatogenesis. At 20 days after the secondary injection, infiltration with neutrophils in these areas had been replaced mostly by mononuclear cells (lymphocytes, plasma cells, and macrophages). Histopathological changes of the testes became severer by further injections until the 10th injection. The EAO lesions in the terminal stage were characterized by complete destruction of the tubular architecture of the testis, fibrosis, and aspermatogenesis. Lesions in the terminal stage were not restored at all. Spermagglutinating antibody titers in the serum increased and delayed-type hypersensitivity against TH estimated by footpad swelling developed in mice injected repeatedly with TH + KO3 LPS. Using immunofluorescence, antibodies against acrosomal components and tail components of the spermatozoa were detected in serum of these mice.
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References
Anderson DJ, Alexander NJ (1981) Antisperm antibody titers, immune complex deposition and immunocompetence in long-term vasectomized mice. Clin Exp Immunol 43:99–108
Bernard CCA, Mitchell GF, Leydon J, Bargerbos A (1978) Experimental autoimmune orchitis in T-cell deficient mice. Int Arch Allergy Appl Immunol 56:256–263
Böhme D (1965) Experimentelle allergische Orchitis and reticulo-endotheliales System. II. Histologische Veränderungen. Z Immunitaets Allergioforsch 128:31–51
Freund J, Lipton MM, Thompson GE (1953) Aspermatogenesis in the guinea pig induced by the testicular tissue and adjuvants. J Exp Med 97:711–725
Hasegawa T, Ohta M, Nakashima I, Kato N, Morikawa K, Harada T, Okuyama T (1985) Structure of the polysaccharide moiety of the Klebsiella O3 lipopolysaccharide isolated from culture supernatant of decapsulated mutant (Klebsiella O3:K1−). Chem Pharm Bull 33:333–339
Iwase K, Fujii Y, Nakashima I, Kato N, Fujino Y, Kawashima H, Mochizuki M (1990) A new method for induction of experimental autoimmune uveoretinitis (EAU) in mice. Curr Eye Res 9:207–216
Kantor GL, Dixon FJ (1972) Transfer of experimental allergic orchitis with exudate cells. J Immunol 108:329–338
Kato N, Ohta M, Kido N, Naito S, Nakashima I, Nagase F, Yokochi T (1985) Strong adjuvanticity of bacterial lipopolysaccharides possessing the homopolysaccharides consisting of mannose as the O-specific polysaccharide chains. Med Microbiol Immunol 174:1–14
Kato T, Yamada H, Yokochi T, Nakashima I, Kato N (1984) Experimental autoimmune hemolytic anemia in mice induced by repeated immunization with ultrasonicated syngeneic erythrocytes together with Klebsiella O3 lipopolysaccharide. Acta Hematol Jpn 47:838–84
Kido N, Nakashima I, Kato N (1984) Correlation between strong adjuvanticity of Klebsiella O3 lipopolysaccharide and its ability to induce interleukin-1 secretion. Cell Immunol 85:477–486
Kido N, Ohta M, Ito H, Naito S, Nagase F, Nakashima I, Kato N (1985) Potent adjuvant action of lipopolysaccharides possessing the O-specific polysaccharide moieties consisting of mannose in antibody response against protein antigen. Cell Immunol 91:52–59
Kohno S, Munoz JA, Williams TM, Teuscher C, Bernard CCA, Tung KSK (1983) Immunopathology of murine experimental allergic orchitis. J Immunol 130:2675–2683
Kojima A, Spencer CA (1983) Genetic susceptibility to testicular autoimmunity: comparison between postthymectomy and postvasectomy models in mice. Biol Reprod 29:195–205
Kosuda LL, Bigazzi PE (1987) Animals models of testis immunity. In: Bigazzi PE (ed) Immunology of the male reproductive system. Marcel Dekker, New York, pp 253–352
Kuriki J, Murakami H, Kakumu S, Sakamoto N, Yokochi T, Nakashima I, Kato N (1984) Experimental autoimmune hepatitis in mice after immunization with syngeneic liver proteins together with the polysaccharide of Klebsiella pneumoniae. Gastroenterology 84:596–603
Nakashima I, Kato N (1975) Microbial adjuvant and autoimmunity. I. Induction of antibody responses to syngeneic tissue extracts in mice treated with capsular polysaccharide of Klebsiella pneumoniae. Japan J Microbiol 19:13–18
Nakashima I, Kobayashi T, Kato N (1972) Adjuvant action of capsular polysaccharide of Klebsiella pneumoniae on antibody response. I. Intensity of its action. J Immunol 108:1009–1016
Nakashima I, Yokochi T, Kato N, Asai J (1977) Microbial adjuvant and autoimmunity. II. Production of lesions in mice immunized with syngeneic tissues extracts together with the capsular polysaccharide of Klebsiella pneumoniae. Microbiol Immunol 27:279–288
Nakashima I, Nagase F, Matsuura A, Yokochi T, Kato N (1980) Adjuvant actions of polyclonal lymphocyte activators. III. Two distinct types of T-initiating adjuvant action demonstrated under different experimental conditions. Cell Immunol 52:429–437
Ohta M, Mori M, Hasegawa T, Nagase F, Nakashima I, Naito S, Kato N (1981) Further studies of the polysaccharide of Klebsiella pneumoniae possessing strong adjuvanticity. I. Production of the adjuvant polysaccharide by noncapsulated mutant. Microbiol Immunol 25:939–948
Ohta M, Nakashima I, Kato N (1982) Adjuvant action of bacterial lipopolysaccharide in induction of delayed-type hypersensitivity to protein antigens. I. Action of the O3 antigen of Klebsiella from culture fluid. Cell Immunol 66:111–120
Ohta M, Nakashima I, Kato N (1982) Adjuvant action of bacterial lipopolysaccharide in induction of delayed-type hypersensitivity to protein antigens. II. Relationships of intensity of the action to that of other immunological activities. Immunobiology 163:460–469
Pokorna Z, Vojtiskova M, Rychikova M, Chunta J (1963) An isologous model of experimental autoimmune aspermatogenesis in mice. Folia Biol (Praha) 9:203–209
Sakaguchi S, Fukuma K, Kuribayashi K, Masuda T (1985) Organ-specific autoimmune diseases induced in mice by elimination of T cell subset. I. Evidence for the active participation of T cells in natural self-tolerance; deficit of a T cell subset as a possible cause of autoimmune disease. J Exp Med 161:72–87
Sato K, Hirokawa K, Hatakeyama S (1981) Experimental allergic orchitis in mice. Histopathological and immunological studies. Virchow Arch [A] 392:147–148
Taguchi O, Nishizuka Y (1981) Experimental autoimmune orchitis after neonatal thymectomy in the mouse. Clin Exp Immunol 46:425–434
Taguchi O, Takahashi T, Seto M, Namikawa R, Matsuyama M, Nishizuka Y (1986) Developement of multiple organ-localized autoimmune diseases in nude mice after reconstitution of T cell function by rat fetal thymus graft. J Exp Med 164:60–71
Teuscher C (1985) Experimental allergic orchitis in mice. II. Association of disease susceptibility with the locus controlling Bordetella pertussis-induced sensitivity to histamine. Immunogenetics 22:417–425
Teuscher C, Smith SM, Goldberg EH, Shearer GM, Tung KSK (1985) Experimental allergic orchitis in mice. I. Genetic control of susceptibility and resistance to induction of autoimmune orchitis. Immunogenetics 22:323–333
Toullet F, Voisin GA (1974) Spermatoxic, spermagglutinating and cytotoxic antibodies of guinea pig autoantibodies to sperm autoantigen. J Reprod Fertil 37:299–313
Toullet F, Voisin GA (1976) Passive transfer of autoimmune aspermogenic orchiepididymitis (AIAO) by antispermatozoa sera. Influence of the type of autoantigen and of the class of antibody. Clin Exp Immunol 26:549–562
Tung KSK, Unanue ER, Dixon FJ (1971) Pathogenesis of experimental allergic orchitis. I. Transfer with immune lymph node cells. J Immunol 106:1453–1462
Tung KSK, Leong C, McCarty T (1977) Pathogenesis of experimental allergic orchitis. III. T lymphocyte requirement in local adoptive transfer by peritoneal exduate cells. J Immunol 118:1774–1779
Voisin GA, Delaunay A, Barber M (1951) Sur des lesions testiculaires provoquees chez le chabaye par iso et autosensitibilisation. Ann Inst Pasteur (Paris) 81:48–63
Waksman BH (1958) A histologie study of the auto-allergic testis lesions in the guinea pig. J Exp Med 109:311–324
Yamaki K, Ohta M, Nakashima I, Noda I, Asai J, Kato N (1980) Microbial adjuvant and autoimmunity. IV. Production of lesions in the exocrine pancreas of mice by repeated injection of syngeneic pancreatic extract together with the capsular polysaccharide of Klebsiella pneumoniae. Microbiol Immunol 24:945–956
Yokochi T, Nakashima I, Kato N, Asai J (1978) Microbial adjuvant and autoimmunity. III. Histological thyroiditis in mice immunized with the syngeneic thyroid extract together with the capsular polysaccharide of Klebsiella pneumoniae. Microbiol Immunol 22:619–630
Yokochi T, Nakashima I, Kato N, Miyadai T, Kimura Y (1986) Microbial adjuvant and autoimmunity. V. The induction of thyroid lesions in syngeneic X-irradiated mice by the transfer of spleen cells from mice immunized with thyroid extract and Klebsiella O3 lipopolysaccharide. Microbiol Immunol 30:553–559
Yokochi T, Inoue Y, Miyadai T, Kimura Y, Kato N (1989) A possible correlation between histological changes in regional subcutaneous tissue induced by bacterial lipopolysaccharides and their adjuvant activities. Microbiol Immunol 33:747–760
Yokochi T, Ikeda H, Inoue Y, Kimura Y, Ito H, Fujii Y, Kato N (1990) Characterization of autoantigens relevant to experimental autoimmune orchitis (EAO) in mice immunized with a mixture of syngeneic testis homogenate and Klebsiella O3 lipopolysaccharide. Am J Reprod Immunol 22:42–48
Yokochi T, Inoue Y, Kimura Y, Kato N (1990) Strong interaction of lipopolysaccharides possessing the mannose homopolysaccharides with complement and its relation to adjuvant action. J Immunol 144:3106–3110
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Fujii, Y., Yokochi, T., Nakashima, I. et al. A new mouse model for autoimmune orchitis. Med Microbiol Immunol 180, 1–14 (1991). https://doi.org/10.1007/BF00191695
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DOI: https://doi.org/10.1007/BF00191695