Abstract
The urinary excretion of free and peptide bound hydroxyproline as well as creatinine and total nitrogen was measured in 198 infants, children, adolescents as well as 11 women aged 20 years. From the data the ratio of urinary hydroxyproline to body weight, creatinine and total nitrogen excretion was calculated from the 1st month of life to the age of 20 years. It is shown that with regard to the above mentioned parameters the largest amount of hydroxyproline is excreted with the urine in the 1st year of life. This also holds true when only the bound hydroxyproline is taken into account. After a significant fall, the relationship between hydroxyproline excretion and body weight, urinary creatinine and nitrogen remains practically constant or decreases minimally between the age of 2 and 20 years. The increased rate of growth during puberty manifests itself in a rapid increase of the absolute value of the excreted hydroxyproline from the age of 9 years onwards. This increase parallels the gain in weight and height (in girls earlier than in boys). At the same time creatinine and nitrogen excretion rises. The absolute hydroxyproline values are considerably lower in girls between the ages of 14 to 17 years than those in boys of the same age.
Zusammenfassung
Bei 198 Säuglingen, Kindern und Adoleszenten sowie bei 11 Frauen im 20. Altersjahr wurde die Ausscheidung von gebundenem und freiem Hydroxyprolin sowie von Kreatinin und Gesamtstickstoff gemessen. Daraus wurde das Verhältnis der Hydroxyprolinwerte zum Körpergewicht, zur Kreatinin- und zur Gesamtstickstoffausscheidung für die Altersstufen vom 1. Lebensmonat bis zum 20. Jahr berechnet. Es wird gezeigt, daß, bezogen auf die genannten Parameter, im 1. Lebenshalbjahr am meisten Hydroxyprolin im Urin ausgeschieden wird. Dies trifft auch zu, wenn nur die gebundene Hydroxyprolinfraktion berücksichtigt wird. Nach einem deutlichen Abfall bleiben die Relationen vom 2. bis zum 20. Altersjahr mehr oder weniger konstant oder sinken nur noch minimal ab. Die Wachstumsbeschleunigung während der Pubertät findet ihren Ausdruck in einer rapiden Zunahme der Absolutwerte des ausgeschiedenen Hydroxyprolins vom 9. Lebensjahr an. Diese Zunahme erfolgt parallel mit dem Gewichtszuwachs (bei den Mädchen früher als bei den Knaben). Ebenso sind Kreatinin- und Stickstoffexkretion in dieser Zeit im Ansteigen begriffen. Die absoluten Hydroxyprolinwerte liegen bei den Mädchen vom 14. bis zum 17. Altersjahr wesentlich tiefer als diejenigen der Knaben in derselben Zeit.
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Literatur
Allison, D. J., Walker, A., Smith, Q. T.: Urinary hydroxyproline: creatinine ratio of normal humans at various ages. Clin. chim. Acta 14, 729 (1966).
Crowne, R. S., Wharton, B. A., McCance, R. A.: Hydroxyproline indices and hydroxyproline/creatinine ratios in older children. Lancet 1969 I, 395.
Dull, T. A., Henneman, P. H.: Urinary hydroxyproline as an index of collagen turnover in bone. New Engl. J. Med. 268, 132 (1963).
Efron, M. L., Bixby, E. M., Pryles, C. V.: Hydroxyprolinemia II. A rare metabolic disease due to a deficiency of the enzyme “hydroxyproline oxidase”. New Engl. J. Med. 272, 1299 (1965).
Folin, O., Wu, H.: A system of blood analysis. J. biol. Chem. 38, 98 (1919).
Hinsberg, K., Lang, K.: In: Medizinische Chemie, S. 964. Berlin-Wien: Urban & Schwarzenberg 1938.
Jasin, H. E., Fink, C. W., Wise, W., Ziff, M.: Relationship between urinary hydroxyproline and growth. J. clin. Invest. 41, 1928 (1962).
Jones, C. R., Bergman, M. W., Kittner, P. J., Pigman, W. W.: Urinary hydroxyproline excretion in normal children and adolescents. Proc. Soc. exp. Biol. (N.Y.) 115, 85 (1964).
Kivirikko, K. J., Laitinen, O.: Clinical significance of urinary hydroxyproline determinations in children. Ann. Paediat. Fenn. 11, 148 (1965).
Klein, L., Teree, T. M.: Skeletal metabolism in early infancy: Urinary hydroxyproline. J. Pediat. 69, 266 (1966).
Laitinen, O., Nikkilä, E., Kivirikko, K. J.: Hydroxyproline in the serum and urine. Acta med. scand. 179, 3 (1966).
Lindstedt, S., Prockop, D. J.: Isotopic studies on urinary hydroxyproline as evidence for rapidly catabolized forms of collagen in the young rat. J. biol. Chem. 236, 1399 (1961).
Matiasevic, D., Gershberg, H.: Studies on hydroxyproline excretion and corticosteroid-induced dwarfism: Treatment with human growth hormone. Metabolism 15, 720 (1966).
McCance, R. A.: Age and renal function. In Black, D. A. K. (ed.): Renal Disease, p. 161. Philadelphia: David Company 1962.
Morrow, G.: Effect of free and peptide hydroxyproline. Pediat. Res. 2, 329 (1968).
—, Kivirikko, K. I., Prockop, D. J.: Catabolism and excretion of free hydroxyproline in infancy. J. clin. Endocr. 27, 1365 (1967).
Neuman, R. E.: Hydroxyproline content of the developing chick embryo. Proc. Soc. exp. Biol. (N.Y.) 76, 289 (1951).
—, Logan, M. A.: The determination of hydroxyproline. J. biol. Chem. 184, 299 (1950).
Picon, D., Alleyne, G. A., Seakin, A.: Hydroxyproline and creatinine excretion in infantile protein malnutrition. Clin. Sci. 29, 517 (1965).
Prockop, D. J.: Isotopic studies on collagen degradation and the urine excretion of hydroxyproline. J. clin. Invest. 43, 453 (1964).
—, Sjoerdsma, A.: Significance of urinary hydroxyproline in man. J. clin. Invest. 40, 843 (1961).
—, Udenfriend, S.: A specific method for the analysis of hydroxyproline in tissues and urine. Analyt. Biochem. 1, 228 (1960).
Saleh, A. E. C., Coenegracht, J. M.: The influence of age and weight on the urinary excretion of hydroxyproline and calcium. Clin. chim. Acta 21, 445 (1968).
Smiley, D. J., Ziff, M.: Urinary hydroxyproline excretion and growth. Physiol. Rev. 44, 30 (1964).
Smith, R., Dehan, M., Reynolds, E. O. R.: Changes in urinary hydroxyproline in premature infants. Clin. chim. Acta 21, 491 (1968).
Weiss, P. H., Klein, L.: The quantitative relationship of urinary peptide hydroxyproline excretion to collagen degradation. J. clin. Invest. 48, 1 (1969).
Whitehead, R. G.: Hydroxyproline creatinine ratio as an index of nutritional status and rate of growth. Lancet 1965 II, 567.
Woolf, L. I., Norman, A. P.: The urinary excretion of amino acids and sugars. J. Pediat. 50, 271 (1957).
Yamamoto, K., Matsuda, I.: Urinary hydroxyproline excretion in normal Japanese children. Clin. chim. Acta 22, 648 (1968).
Younoszai, M. K., Andersen, D. W., Filer, L. J., Fomon, S. J.: Urinary excretion of endogenous hydroxyproline by normal male infants. Pediat. Res. 1, 266 (1967).
—, Haworth, J. C.: Excretion of hydroxyproline in urine by premature and normal full-term infants and those with intrauterine growth retardation during the first three days of life. Pediat. Res. 2, 17 (1968).
Ziff, M., Kibrick, A., Dresner, E., Gribetz, H. J.: Excretion of hydroxyproline in patients with rheumatic and non-rheumatic diseases. J. clin. Invest. 35, 579 (1956).
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Graf, U., Vest, M. Zur normalen Hydroxyprolinausscheidung im Urin bei Säuglingen, Kindern und Adoleszenten. Z. Kinder-Heilk. 110, 128–139 (1971). https://doi.org/10.1007/BF00442287
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DOI: https://doi.org/10.1007/BF00442287