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Characterization of serum alkaline phosphatase in infancy and childhood

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Abstract

In 364 serum samples of cord blood, infants, children, and adults alkaline phosphatase was characterized by means of L-phenylalanine inhibition, L-homoarginine inhibition, heat inactivation, acrylamide gel disc electrophoresis, and neuraminidase sensitivity. The increase of serum alkaline phosphatase in cord blood serum and in infant's and children's serum in comparison with adult levels was attributed mainly to the bone enzyme and in smaller proportion to the intestinal enzyme. Using acrylamide gel disc electrophoresis, an increase in the frequency of subjects with an intestinal alkaline phosphatase band with increasing age values was found.

Zusammenfassung

In 364 Seren von Neugeborenen (Nabelschnurblut), Säuglingen, Kindern und Erwachsenen wurde die vorhandene alkalische Phosphatase genauer differenziert unter Anwendung der L-Phenylalaninhemmung, der L-Homoargininhemmung, der Hitzedenaturierung, der Arcylamidgel-Scheibenelektrophorese und der Neuramidaseempfindlichkeit. Die hohen Werte der alkalischen Phosphatase im Nabelschnur-, Säuglings-und Kinderserum im Vergleich zu den Erwachsenenwerten werden mehr den Phosphatasen der Knochen als den intestinalen Phosphatasen zugerechnet. Mit Hilfe der Acrylgelelektrophorese konnte eine Zunahme der intestinalen alkalischen Phosphatase mit steigendem Lebensalter nachgewiesen werden.

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Authors and Affiliations

  1. Clinic of Paediatrics, University of Perugia, Perugia, Italy

    A. Cao, G. Coppa, N. Trabalza, F. Marcucci, S. de Virgiliis, S. Lungarotti & M. Furbetta

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  1. A. Cao
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  2. G. Coppa
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  3. N. Trabalza
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  4. F. Marcucci
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  5. S. de Virgiliis
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  6. S. Lungarotti
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  7. M. Furbetta
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This work was supported by the National Research Council.

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Cao, A., Coppa, G., Trabalza, N. et al. Characterization of serum alkaline phosphatase in infancy and childhood. Z. Kinder-Heilk. 113, 289–296 (1972). https://doi.org/10.1007/BF00440307

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  • DOI: https://doi.org/10.1007/BF00440307

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