Abstract
Twenty-one protein components were measured in urine specimens from 12 subjects with postural proteinuria in the recumbent posture and after a period of upright lordotic posture. The lordotic posture produced an increased urinary excretion of total protein and 19 protein components, particularly of albumin, IgG globulin, and transferrin (128, 68, and 167-fold, respectively). However, 90 min after the lordotic posture, most of the protein components returned to initial values, whereas albumin, IgG globulin, and transferrin remained high. β2-Microglobulin, retinol binding protein, and lysozyme showed the smallest increases after the lordotic posture. These findings suggest that proteinuria occurring after the lordotic posture may be characterized by an increased glomerular permeability.
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Bauman JW (1981) On the mechanism of angiotensin-induced proteinuria. I. Studies in aminonucleoside nephrotic rats and in saralasin blockade. Nephron 27:47–50
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Ellis D, Buffone GJ (1977) New approach to evaluation of proteinuric states. Clin Chem 23:666–670
Frey FJ, Frey BM, Koegel R, Hodler J (1979) Selectivity as a clue to diagnosis of postural proteinuria. Lancet 1:343–345
Hardwicke J (1975) Laboratory aspects of proteinuria in human disease. Clin Nephrol 3:37–41
Johansson BG, Ravnskov U (1972) The serum level and urinary excretion of α2-microglobulin, β3-microglobulin and lysozyme in renal disease. Scand J Urol Nephrol 6:249–256
King SE (1952) Benign and pathologic albuminuria: A study of 600 hospitalized cases. Ann Intern Med 36:765–785
King SE, Baldwin DS (1954) Renal hemodynamics during erect lordosis in normal man and subjects with orthostatic proteinuria. Proc Soc Exp Biol Med 86:634–636
Manuel Y, Colle A, Leclercq M, Tonnelle C (1975) Low molecular weight proteinuria. Contrib Nephrol 1:156–162
Mckay E, Slater RJ (1962) Studies on human proteinuria. II. Some characteristics of the gamma globulins excreted in normal, exercise, postural, and nephrotic proteinuria. J Clin Invest 41: 1638–1652
Ogura T, Tomiyama T, Kimura M (1979) Determination of β2-microglobulin by reverse passive hemagglutination. Igakuno Ayumi 108:31–33
Pabico RC, Cala AR, McKenna BA, Freeman RB (1977) Massive proteinuria and its effects on renal tubular functions. Proc Eur Dial Transplant Assoc 14:495–500
Peterson PA, Berggård I (1971) Urinary immunoglobulin components in normal ‘tubular’ and glomerular proteinuria: Quantities and characteristics of free light chains, IgG, IgA, and Fcγ fragment. Europ J Clin Invest 1:255–264
Peterson PA, Evrin PE, Berggård I (1966) Differentiation of glomerular, tubular, and normal proteinuria: Determinations of urinary excretion of β2-microglobulin, albumin, and total protein. J Clin Invest 48:1189–1198
Poortmans J, Jeanloz RW (1968) Quantitative immunological determination of 12 plasma proteins excreted in human urine collected before and after exercise. J Clin Invest 47:386–393
Robinson RR, Glenn WG (1964) Fixed and reproducible orthostatic proteinuria. IV. Urinary albumin excretion by healthy human subjects in the recumbent and upright postures. J Lab Clin Med 64:717–723
Robinson RR, Glover SN, Phillippi PJ, Lecocq FR, Langelier PR (1961) Fixed and reproducible orthostatic proteinuria. I. Light microscopic studies of the kidney. Am J Pathol 39:291–306
Robinson RR, Ashworth CT, Glover SN, Phillippi PJ, Lecocq FR, Langelier PR (1961) Fixed and reproducible orthostatic proteinuria. II. Electron microscopy of renal biopsy specimens from five cases. Am J Pathol 39:405–417
Robinson RR, Lecocq FR, Phillippi PJ, Glenn WG (1963) Fixed and reproducible orthostatic proteinuria. III. Effect of induced renal hemodynamic alterations upon urinary protein excretion. J Clin Invest 42:100–110
Rowe DS, Soothill JF (1961) The proteins of postural and exercise proteinuria. Clin Sci 21:87–91
Sinniah R, Law CH, Pwee HS (1977) Glomerular lesions in patients with asymptomatic persistent and orthostatic proteinuria discovered on routine medical examination. Clin Nephrol 7:1–14
Suzuki Y, Okada T, Konishi T, Higuchi A, Shimao S, Ogita Z (1980) The low molecular weight of protein components in children urine. Acta Pediatr Jap 22:1–5
Terano Y, Shibata K, Ichihashi H (1973) Further studies on single radial immunodiffusion in antibody-contained gel and antigen-contained gel layers. Jap J Clin Chem 2:28–55
Watson A (1951) Orthostatic albuminuria. A time-saving method of diagnosis. Lancet 1:1196–1197
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Suzuki, Y., Shimao, S., Okada, T. et al. Quantitative determination of urinary protein components of children with postural proteinuria. Eur J Pediatr 140, 268–272 (1983). https://doi.org/10.1007/BF00443374
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DOI: https://doi.org/10.1007/BF00443374