Summary
Human adult peripheral blood lymphocytes were successfully immortalized by co-cultivation with irradiated autologous and homologous T-cell lines harbouring human T-cell leukaemia virus-I (HTLV-I). The efficiency of transformation was the same in both cases. The participation of alloantigen stimulation in co-cultivation procedures is discussed, and it is stressed that factors other than alloantigen stimulation might be required for the efficient immortalization of human T-lymphocytes in vitro by HTLV.
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Akagi, T., Ohtsuki, Y., Takahashi, K. et al. Immortalization of human lymphocytes by co-cultivation with lethally irradiated autologous T-cell lines harbouring human T-cell leukaemia virus-I. J Cancer Res Clin Oncol 110, 82–84 (1985). https://doi.org/10.1007/BF00402508
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DOI: https://doi.org/10.1007/BF00402508