Summary
The expression of cytokine genes for tumor necrosis factorα (TNFα), lymphotoxin and transforming growth factorβ (TGFβ), all of which are known to suppress normal hematopoiesis, was investigated in 32 patients with lymphoid malignancies using Northern blot analysis. Messenger RNA (mRNA) for TNFα, lymphotoxin and TGFβ was detected in 9 cases, 2 cases and 7 cases, respectively. When the relationship between cytokine gene expression and surface phenotype was analyzed, the expression of CD19 correlated significantly with expression of the TNFα gene (P<0.05). This suggests that B cell malignancies are likely to produce TNFα. When the hematological parameters of patients expressing and not expressing the gene were compared, the expression of TNFα mRNA was found to correlate with more profound anemia in acute lymphoblastic leukemia (P<0.05). Both granulocyte and platelet counts were lower in patients expressing TNFα mRNA; however, the decreases were not significant. Neither lymphotoxin nor TGFβ gene expression correlated significantly with any hematological parameter.
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Abbreviations
- TNFα :
-
tumor necrosis factorα
- TGFβ :
-
transforming growth factorβ
- IL:
-
interleukin
- ALL:
-
acute lymphoblastic leukemia
- CLL:
-
chronic lymphocytic leukemia
- ATLL:
-
adult T-cell leukemia/lymphoma
- NHL:
-
non-Hodgkin's lymphoma
- MM:
-
multiple myeloma
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Partly supported by grants-in-aid from the Ministry of Education, Science and Culture of Japan (60770949, 63015063, 02256102, 03670325) and from the Fukuoka Anti-Cancer-Society.
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Kawasaki, C., Okamura, S., Hayashi, S. et al. Gene expression of cytokines suppressing hematopoietic progenitor cells in lymphoid malignancies. J Cancer Res Clin Oncol 117, 359–363 (1991). https://doi.org/10.1007/BF01630720
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DOI: https://doi.org/10.1007/BF01630720