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Swelling of normal, preneoplastic and neoplastic liver mitochondria

I. Spontaneous and thyroxine-induced swelling of normal liver mitochondria as affected by succinate and adenine nucleotides

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Summary

The swelling of rat and mouse liver mitochondria, isolated in 0.30 M sucrose and incubated in sucrose-Tris of pH 7.4 at 22±1°C, in response to succinate, adenine nucleotides (10−5 M), malonate, thyroxine, and DNP, separately or in various combinations, was measured.

It was found that both the spontaneous and thyroxine-induced swelling of fresh mitochondria were counteracted by succinate and, to a still greater extent, by succinate plus adenine nucleotide (ADP≥ATP≫ or > AMP). The protection was abolished by malonate or DNP. The protection against swelling afforded by succinate to fresh mitochondria was followed by a phase of rapid swelling. Progressive ageing or frequent washing of the particles, prior to their incubation, led to a reduction or abolition of the protective effect. It was concluded that mitochondrial swelling was governed by the degree of intrinsic stability of the isolated particles.

Zusammenfassung

Die Schwellung von in 0,30 M Saccharose isolierten und in Saccharose-Tris von pH 7,4 bei 22±1°C inkubierten Mitochondrien aus Mäuse- und Rattenlebern wurde gemessen nach Zusatz von Succinat, Adeninnucleotiden (10−5 M), Malonat, Thyroxin und DNP, allein und in verschiedenen Kombinationen. Sowohl die spontane wie die thyroxininduzierte Schwellung frischer Mitochondrien wird durch Succinat und noch mehr durch Succinat und Adeninnucleotide (ADP≥ATP≫ oder > AMP) gehemmt. Die Hemmung wird aufgehoben durch Malonat oder DPN. Auf die Hemmung der Schwellung von frischen Mitochondrien durch Succinat folgt eine Phase schneller Schwellung. Allmähliche Alterung oder wiederholte Waschungen von den Mitochondrien vor der Inkubation führen zu einer Verringerung oder Aufhebung des hemmenden Effektes des Succinats.

Daraus wird gefolgert, daß die mitochondriale Schwellung von dem Grade der Eigenstabilität der isolierten Partikel abhängig ist.

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Abbreviations

O.D.520 :

optical density at 520 mμ

EDTA:

ethylenediamine tetra-acetate

AMP, ADP and ATP:

adenosine mono-,-di- and-triphosphates

Tris:

2-amino-2-hydroxymethylpropane-1:3-diolchloride

DPN:

diphosphopyridine nucleotide

DNP:

2:4-dinitrophenol

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Authors and Affiliations

  1. Department of Biochemistry, Antoni van Leeuwenhoek-Huis, The Netherlands Cancer Institute, Amsterdam, The Netherlands

    P. Emmelot, C. J. Bos & I. H. M. Reyers

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  1. P. Emmelot
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  2. C. J. Bos
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  3. I. H. M. Reyers
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Emmelot, P., Bos, C.J. & Reyers, I.H.M. Swelling of normal, preneoplastic and neoplastic liver mitochondria. Z Krebs-forsch 64, 22–34 (1960). https://doi.org/10.1007/BF00524274

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