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Experimental investigations on the influence upon the chemical carcinogenesis

IIIrd communication: Studies with 1,2-dimethylhydrazine

Experimentelle Untersuchungen zur Beeinflussung der chemischen Carcinogenese

III. Mitteilung: Versuche mit 1,2-Dimethylhydrazin

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Summary

After ten times monthly, subcutaneous, injections of 30mg/kg 1,2-dimethylhydrazine (DMH) to Sprague-Dawley-rats, malignant tumors were found in ∼90% of the animals after a mean induction period of ∼330 days. Injections were started on the 2nd day of life and maintained during 10 months. 72% of the tumors induced were formed in the colon, 17% were squamous cell carcinomas of the ear duct, adenosarcomas of the kidney and hepatocellular carcinomas were found in 13 and 11% of the animals, respectively. Additional treatment with immunodepressive (cyclophosphamide, methotrexat, hydrocortisone) and immunostimulating substances (BCG, albumin, vitamin A-acid) as well as an enzyme-stimulating agent (Luminal) did not alter incidences and induction periods of tumors. After application of a vegetarian diet, the rate of liver and kidney tumors was diminished significantly and induction periods of intestinal and ear duct tumors increased.

Zusammenfassung

Nach zehnmaliger, monatlicher, subcutaner Injektion von 30 mg/kg 1,2-Dimethylhydrazin an Sprague-Dawley-Ratten, beginnend am 2. Lebenstag, entwickelten ∼90% der Tiere nach ∼330 Tagen Adenocarcinome des Dickdarms (72%), Plattenepithelcarcinome des Gehörgangs (17%). Adenosarkome der Niere (13%) sowie hepatocelluläre Carcinome (11%). Zusatzbehandlungen mit Immunodepressiva (Cyclophosphamid, Methotrexat, Hydrocortison), Immunostimulantien (BCG, Albumin, Vitamin A-Säure) oder Enzymstimulatoren (Luminal) änderten die Tumorausbeuten und Induktionszeiten nicht. Nach Applikation einer vegetarischen Diät waren die Ausbeuten an Nieren- und Leberkrebsen signifikant vermindert und die Induktionszeiten der Darm- und Gehörgangskrebse vergrößert.

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Dedicated to Prof. E. Boyland (London), on the occasion of his 70th birthday.

We are indebted to Mr. Hans Hausberger and Mr. Hans-Joachim Verges, for financial support of our work.

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Schmähl, D., Danisman, A., Habs, M. et al. Experimental investigations on the influence upon the chemical carcinogenesis. Z. Krebsforsch. 86, 89–94 (1976). https://doi.org/10.1007/BF00304938

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  • DOI: https://doi.org/10.1007/BF00304938

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