Summary
Five subjects were injected with 5,000 IU of commercial heparin and low-molecular-weight heparin at an interval of 20 days after each injection. Both heparins produced the same platelet factor 4 release immediately after administration (commercial heparin 114.6 ± 21.6ng/ml, low-molecular-weight heparin 113.1 ± 22.1 ng/ml). However, commercial heparin induced a more evident potentiating effect on ADP-induced platelet aggregation and was still present 60min after injection. Low-molecular-weight heparin had a higher anti-Xa-specific activity than that determined by activated partial thromboplastin time. The opposite was true for the commercial preparation.
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References
Lane DA, McGregor RI, Van Ross M, Cella G, Kakkar VV (1979) Molecular weight dependence of the anticoagulant properties of heparin: Intravenous and subcutaneous administration of fractionated heparins to man. Thromb Res 16:651–662
Fussi F, Girolami A, Cella G (1980) Low-molecular-weight heparins and their effects in vitro, in experimental models and in humans. In: Lundblad RL, Brown WV, Mann KG, Roberts HR (eds) Chemistry and biology of heparin. Elsevier North-Holland Amsterdam, pp 535–546
Salzmann EW, Rosemberg RD, Smith MH, Lindon JN, Favreau L (1980) Effect of heparin and heparin fractions on platelet aggregation. J Clin Invest 65:64–73
Fabris F, Fussi F, Casonato A, Visintin L, Randi ML, Smith MR, Girolami A (1983) Normal and low-molecular-weight heparins: Interaction with human platelets. Eur J Clin Invest 13:135–139
Cella G, Menardo A, Girolami A (1980) Effect of heparin on in vivo platelet factor 4 (PF4) release and platelet aggregation after aspirin administration. Clin Lab Haematol 2:333–338
Files JC, Malpass TW, Yee EK, Ritchie JL, Harker LA (1981) Studies of human platelet alpha-granule release in vivo. Blood 58:607–617
Friberger P (1983) Properties of the Coatest heparin assay. In: Witt I (ed) Heparin. New biochemical and medical aspects. De Gruyter, Berlin New York, pp 117–127
Girolami A, De Marco L, Virgolini L, Peruffo R, Fabris F (1975) Platelet adhesiveness and aggregation in congenital afibrinogenemia. Blut 30:87–100
Handin RI, Cohen H (1976) Purification and binding properties of human platelet factor four. J Biol Chem 251:4273–42182
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Supported by grants from the CNR, Rome (grant no. 82. 02193.56), the MPI, Rome (grant no. 1952–1980), and the Venetian Regional Government, Venice, Italy
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Cella, G., Scattolo, N., Luzzatto, G. et al. Effects of low-molecular-weight heparin on platelets as compared with commercial heparin. Res. Exp. Med. 184, 227–229 (1984). https://doi.org/10.1007/BF01852381
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DOI: https://doi.org/10.1007/BF01852381