Summary
Beside traditional techniques (light, immunofluorescence, transmission electron (TEM)- and scanning electron microscopy (SEM)), a new more sensitive method for producing microcorrosion casts using a polyesterbased resin has been developed. The SEM analyses of the microcorrosion casts of alloplastic vascular grafts was realized in an absolutely stable stage of polymerization. For the first time, a transprosthetic vascularization could be shown in great three-dimensional detail. The importance of a complete and rapid endothelialization of artificial vascular grafts is discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Amselgruber W, König HE (1987) Vereinfachte Herstellung von Gefäßkorrosionspräparaten zur rasterelektronenmikroskopischen Untersuchung. Z Mikrosk Anat Forsch 101:523–531
Branson DF, Picha GJ, Desprez J (1985) Expanded polytetrafluoroethylene as a microvascular graft: A study of four fibril lengths. Plast Reconstr Surg 76:754–763
Bugge J (1963) A standardized plastic injection technique for anatomical purposes. Acta Anat (Basel) 54:177–192
Clowes AW, Gown AM, Hanson SR, Reidy MA (1985) Mechanisms of arterial graft failure. Am J Pathol 118:43–54
Clowes AW, Kirkman TR, Reidy MA (1986) Mechanisms of arterial graft healing. Am J Pathol 123:220–230
Cyba-Altunbay S (1986) Der Kunststoffersatz großer Körpervenen — eine tierexperimentelle Studie. Habilitationsschrift, Universität Ulm
Cyba-Altunbay S, Vollmar JF (1987) Der Kunststoffersatz großer Körpervenen. VASA [Suppl] 19:1–29
Florey HW, Greer SJ, Kiser J, Poole JCF, Telander R, Werthhessen NT (1962) The development of the pseudointima lining fabric grafts of the aorta. Br J Exp Pathol 43:655–660
Heyden B (1979) Der Ersatz großer Körpervenen — neue Aspekte aus tierexperimentellen Untersuchungen. Habilitationsschrift, Universität Ulm
Kogel H, Cyba-Altunbay S (1987) Das Vena cava inferior-Modell als Prüfstein für den Einsatz optimierter, biokompatibler Venenprothesen. Acta Chir Austriaca, 2:19
Kogel H, Cyba-Altunbay S, Pindur G, Seifried E, Ostermeyer U (1988) Thrombogenität von künstlichen Blutleitern — ein modifiziertes in vivo-Prüfmodell. VASA 17:177–186
Kusaba A, Fischer CR, Matulewski TJ, Matsumoto T (1981) Experimental study of the influence of porosity on development of neointima in Gore-Tex grafts: A method to increase long-term patency rate. Am Surg 47:347–354
Murakami T (1971) Application of the scanning electron microscope to the study of the fine distribution of the blood vessels. Arch Histol Jpn 32:445–454
Murakami T (1972) Vascular arrangement of the rat renal glomerulus. A scanning electron microscope study of corrosion casts. Arch Histol Jpn 34:87–107
Nowell JA, Lohse CL (1974) Injection replication of the microvasculature for SEM. In: Johari O, Corvin I (eds) Scanning electron microscopy. IIT Research Institute, Chicago, p 217
Schummer A (1935) Ein neues Mittel (Plastoid) und Verfahren zur Herstellung korrosionsanatomischer Präparate. Anat Anz 81:177–201
Schummer A (1936) Korrosionsanatomische Präparate mit neuem Verfahren hergestellt. Anat Anz 81:301
Vollmar JF (1986) Robert May memorial lecture: Advances in reconstructive venous surgery. Int Angiol 5:117–129
Voss EU (1981) Künstliche Blutleiter zum Ersatz großer Körpervenen. Habilitationsschrift, Universität Ulm
Wesolowsky SA (1972) Performance of materials as prosthetic blood vessels. Bull NY Acad Med 48:331–356
Author information
Authors and Affiliations
Additional information
Dedicated to Professor J. F. Vollmar, MD, on the occasion of his 65th birthday
Supported financially by the BMFT/GSF (BIM Phase II, Nr. 0702140/1)
Rights and permissions
About this article
Cite this article
Kogel, H., Amselgruber, W., Frösch, D. et al. New techniques of analyzing the healing process of artificial vascular grafts, transmural vascularization, and endothelialization. Res. Exp. Med. 189, 61–68 (1989). https://doi.org/10.1007/BF01856031
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01856031