Summary
The Indirect Fluorescent Antibody Technique (IFAT) and the Indirect Immuno Peroxidase Technique (IIPT) have been applied to cryostat sections and intact stages of the hookworm speciesAncylostoma caninum with sera from infected dogs. Especially the role of the body surface (=cuticle (cortex, matrix, basal layer) and hypodermis) in immunity was studied. Using cryostat sections and dead intact stages as the antigen, specific antibody binding was demonstrated round the ovum membrane and the cuticle of all stages of this hookworm species. Cryostat sections of adult worms showed, that it probably is not the cuticle itself that is antigenic, but that the specific reaction that is observed consisted of a layer, covering the cortex of the cuticle. Infective and parasitic living stages, however, showed no antibody binding in contrast to the free-living stages in which specific antibody binding was demonstrated.
Article PDF
Similar content being viewed by others
References
Azevedo, J. Fraga de, Rombert, P.: Antigen factors that can interfere with results of immunofluorescence tests in some helminthiasis. In: Standardization in immunofluorescence. E.J. Holborow, ed., Oxford and Edingburgh: Blackwell 1970
Ball, P.A.J., Bartlett, A.: Serological reactions to infection withNecator americanus. Trans. R. Soc. Trop. Med. Hyg.63, 362–369 (1969)
Bird, A.F.: Further observations on the structure of the nematode cuticle. Parasitology48, 32–39 (1958)
Clegg, J.A., Smithers, S.R.: The effects of immune rhesus monkey serum on schistosomula ofSchistosoma mansoni during cultivation in vitro. Int. J. Parasitol.2, 79–88 (1972)
Goldman, M.: Fluorescent antibody methods. New York: Academic Press 1968
Hogarth-Scott, R.S.: Naturally occuring antibodies to the cuticle of nematodes. Parasitology58, 221–226 (1968)
Lee, D.L.: The structure and composition of the helminth cuticle. Adv. Parasitol.4, 187–254 (1966)
Lumsden, R.D.: Parasitological review. Surface ultrastructure and chemistry of parasitic helminths. Exp. Parasitol.37, 267–339 (1975)
Miller, T.A.: Vaccination against the canine hookworm diseases. Adv. Parasitol.9, 153–183 (1971)
Otto, G.F., Kerr, K.B.: The immunization of dogs against hookworm,Ancylostoma caninum, by subcutaneous injection of graded doses of living larvae. Am. J. Hyg.29, 25–45 (1939)
Ourth, D.D., Matre, R., Helgeland, S.M., Tönder, O: An indirect immuno peroxydase test for human antibodies toToxoplasma gondii. Acta Pathol. Microbiol. Scand.82, 145–150 (1974)
Owen, J., Slocombe, D., Whitlock, J.H.: Rapid ecdysis of infectiveHaemonchus contortus cayugensis larvae. J. Parasitol.55, 1102–1104 (1969)
Pearse, A.G.E.: Histochemistry, Vol 2. p. 1438 Edingburgh and London: Churchill Livingstone 1972
Seesee, F.M., Wescott, R.B., Gorham, J.R.:Nippostrongylus brasiliensis: indirect fluorescent antibody studies of immunity in mice. Exp. Parasitol.39, 214–221 (1976)
Sinclair, I.J.: The relationship between circulating antibodies and immunity to helminthic infections. Adv. Parasitol.8, 97–127 (1970)
Swellengrebel, N.H., Sterman, M.M.: Animal parasites in man. Princeton, N.J.: Van Nostrand 1961
Zaman, V., Singh, M.: Immuno-fluorescent studies with hookworms. I. Antigenic relationships of ova. Trans. R. Soc. Trop. Med. Hyg.59, 690–693 (1965)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Klaver-Wesseling, J.C.M., Vetter, J.C.M. & Visser, W.K. A comparative in vitro study of antibody binding to different stages of the hookwormAncylostoma caninum . Z. Parasitenkd. 56, 147–157 (1978). https://doi.org/10.1007/BF00930745
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00930745