Summary
A new photoactivable reagent is described, which allows the formation of RNA-protein crosslinks via disulfide bridges in combination with mercaptobutyrimidate.
The reconstituted L24 protein-23S RNA complex from the large subunit of E. coli ribosomes has been used as a model system for the cross-linking. The main advantages of the reagent are the absence of U.V. generated cross-links, since photoactivation is carried out at 360 nm, on one hand and the ease of cleavage of the cross-link by mild reduction (β-mercaptoethanol) on the other.
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Communicated by H. G. Wittmann
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Oste, C., Parfait, R., Bollen, A. et al. A new nucleic acid-protein cross-linking reagent. Molec. Gen. Genet. 152, 253–257 (1977). https://doi.org/10.1007/BF00693078
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DOI: https://doi.org/10.1007/BF00693078