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Geentic organization of the E. coli chromosome around the structural gene for initiation factor IF3 (infC)

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Summary

A set of λ transducing phages carrying varying lengths of the E. coli chromosome around the structural gene for initiation factor IF3 (infC) was derived from λp2 which is known to cary, besides infC, the structural genes for the α subunit of phenylalanyl-tRNA synthetase (pheS), the β subunit of phenylalanyl-tRNA synthetase (phetT) and the structural gene for threonyl-tRNA synthetase (thrS). The E. coli coding content of these derived phages was analysed by genetic complementation of a set of mutants and by SDS-polyacrylamide gel analysis of the proteins synthesized in UV irradiated cells infected with these phages. The segregation pattern of the different genes among these derived phages indicates that the order of the genes is pheT-pheS-“P12”-(infC, thrS) where infC is probably between “P12” and thrS. “P12” is the structural gene of a 12,000 molecular weight unidentified protein.

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Abbreviations

PRS (EC 6.1.1.20):

phenylalanyl-tRNA synthetase

TRS (EC 6.1.1.3):

threonyl-tRNA synthetase

IF3:

Initiation factor IF3

SDS:

Sodium dodecyl sulfate

PPR :

pyrophosphate resistant

PPS :

pyrophosphate sensitive

References

  • Böck, A., Neidhardt, F.C.: Genetic mapping of phenylalanyl-sRNA synthetase in E. coli. Science 157, 78–79 (1967)

    Google Scholar 

  • Comer, M.M., Böck, A.: Genes for the α and β subunits of the phenylalanyl transfer ribonucleic Acid Synthetase of Escherichia Coli. J. Bacteriol. 127, 923–933 (1976)

    Google Scholar 

  • Davis, R.W., Parkinson, J.S.: Deletion mutants of bacteriophage λ. III Physical structure of att ϕ. J. Mol. Biol. 56, 403–423 (1971)

    Google Scholar 

  • Epstein, W., Kim, B.S.: Protassium transport loci in E. coli K12. J. Bacteriol. 108, 639–644 (1971)

    Google Scholar 

  • Hennecke, H., Böck, A., Thomale, J., Nass, G.: Threonyl-transfer ribonucleic acid synthetase from E. coli: subunit structure and genetic analysis of the structural gene by means of a mutated enzyme and of a specialized transducing λ bacteriophage. J. Bacteriol. 131, 943–950 (1977b)

    Google Scholar 

  • Hennecke, H., Springer, M., Böck, A.: A specialized transducing λ phage carrying the E. coli genes for phenylalanyl-tRNA synthetase. Mol. Gen. Genet. 152, 205–210 (1977a)

    Google Scholar 

  • Jaskunas, S.R., Lindahl, L., Nomura, M., Burgess, R.R.: Identification of two copies of the gene for the elongation factor EF-Tu in E. coli. Nature 257, 458–462 (1975)

    Google Scholar 

  • Johnson, E.J., Cohen, G., Saint-Girons, I.: Threonyl-transfer ribonucleic acid synthetase and the regulation of the threonine operon in E. coli. J. Bacteriol. 129, 66–70 (1977)

    Google Scholar 

  • Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685 (1970)

    PubMed  Google Scholar 

  • Miller, J.H.: Experiments in molecular genetics. Cold spring Harbor, N.Y.. Cold spring Harbor laboratory 1972

    Google Scholar 

  • Nomura, M., Morgan, E.A., Jaskunas, S.R.: Genetics of bacterial ribosomes. In: Ann. Rev. Genet. Vol. 11, pp. 297–347. Palo Alto: Annual reviews inc. 1977

    Google Scholar 

  • Parkinson, J.S., Huskey, R.J.: Deletion mutants of bacteriophage λ. I. Isolation and initial characterization. J. Mol. Biol. 56, 369–384 (1971)

    PubMed  Google Scholar 

  • Russel, R.R.B., Pittard, A.J.: Mutants of E. coli unable to make protein at 42°C. J. Bacteriol. 108, 790–798 (1971)

    Google Scholar 

  • Shrenk, W.J., Weisberg, R.A.: A simple method for making new transducing lines of coliphage lambda. Mol. Gen. Genet. 137, 101–107 (1975)

    Google Scholar 

  • Springer, M., Graffe, M., Grunberg-Manago, M.: Characterization of an E. coli mutant with a thermolabile initiation factor IF3 activity. Mol. Gen. Genet. 151, 17–26 (1977a)

    Google Scholar 

  • Springer, M., Graffe, M., Hennecke, H.: Specialized transducing phage for the initiation factor IF3 gene in E. coli. Proc. Natl. Acad. Sci. USA 74, 3970–3974 (1977b)

    Google Scholar 

  • Suryanarayana, T., Subramanian, A.R.: Separation of two forms of IF3 in Escherichia Coli by two-dimensional gel electrophoresis. FEBS Letters 79, 264–268 (1977)

    Google Scholar 

  • Taylor, A.L., Thoman, M.S.: The genetic map of E. coli K12 Genetics 50, 659–677 (1964)

    Google Scholar 

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Authors and Affiliations

  1. Institut de Biologie Physico-Chimique, 13, rue Pierre et Marie Curie, F-75005, Paris, France

    M. Springer, M. Graffe & M. Grunberg-Manago

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  1. M. Springer
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  2. M. Graffe
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  3. M. Grunberg-Manago
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Communicated by H.G. Wittmann

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Springer, M., Graffe, M. & Grunberg-Manago, M. Geentic organization of the E. coli chromosome around the structural gene for initiation factor IF3 (infC). Molec. Gen. Genet. 169, 337–343 (1979). https://doi.org/10.1007/BF00382279

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  • DOI: https://doi.org/10.1007/BF00382279

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