Summary
The occurrence of “up promoter” mutations appears to be a general feature of eukaryotic gene regulation. Such mutations have been described in organisms as different as Yeast, Aspergillus, and Drosophila (Douglas and Hawthorne, 1966; Arst and Scazzochio, 1975; Hynes, 1975; Ciriacy, 1976; Chovnick et al., 1976), yet little is known about the underlying regulatory mechanisms.
Some years ago, Clavilier et al. (Clavilier et al., 1969) discovered in S. cerevisiae regulatory mutations enhancing the synthesis of iso-2 cytochrome c. One of these mutations was found to entail a secondary phenotype prohibiting growth on a glucose medium containing high levels of chloramphenicol (Péré-Aubert, 1970).
In this paper evidence is presented that:
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1.
This regulatory mutation, CYP3-15, is linked to the structural gene of iso-2 cytochrome c, is cis dominant, and therefore qualifies as an “up promoter” mutation of the iso-2 cytochrome c producing unit.
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2.
The secondary phenotype entailed by CYP3-15 never occurs in the absence of the main phenotype of iso-2 cytochrome c stimulation, and is a consequence of the extension into a nearby function of the genetic alteration corresponding to CYP3-15. This secondary phenotype is manifest whatever the genotype at the CYC1 locus, including in the presence of the wild type allele.
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3.
Despite being cis dominant, CYP3-15 is still receptive to the effect of three other independent regulatory mutations. One of these, which reduces both the iso-1 and iso-2 cytochrome c level, is a null allele of the CYP1 locus. This later locus was also originally identified by mutations which enhance, to various degrees, the amount of iso-2 cytochrome c produced by the cell (Clavilier et al., 1976).
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References
Arst, H., Scazzochio, G.: Initiator constitutive mutation with an “up promotor” effect in Aspergillus nidulans. Nature 254, 31–34 (1975)
Chovnick, A., Celbart, W., McCarron, M., Osmond, B., Candido, E.P., Baillie, D.L.: Organisation of the rosy locus in Drosophila melanogaster: Evidence for a control element adjacent to the xantine deshydrogenase structural element. Genetics 84, 233–255 (1976)
Ciriacy, M.: Cis dominant regulatory mutations affecting the formation of glucose repressible alcohol dehydrogenase in Saccharomyces cerevisiae. Mol. Gen. Genet. 145, 327–333 (1976)
Claisse, M., Péré-Aubert, G., Clavilier, L., Slonimski, P.P.: Méthode d'estimation de la concentration de cytochromes dans les cellules entières de levure. Eur. J. Biochem. 16, 430–438 (1970)
Clavilier, L., Péré-Aubert, G., Slonimski, P.P.: Mise en évidence de plusieurs loci indépendants impliqués dans la synthèse de l'iso-2-cytochrome c chez la levure. Mol. Gen. Genet. 104, 195–218 (1969)
Clavilier, L., Péré-Aubert, G., Somlo, M., Slonimski, P.P.: Réseau d'interactions entre des gènes non liés: régulation synergique ou antagoniste de la synthèse de l'iso-1 cytochrome c, de l'iso-2 cytochrome c et du cytochrome b2. Biochimie 58, 155–172 (1976)
Dubois, E., Herniaux, D., Grenson, M., Wiam, J.M.: Specific induction of catabolism and its relation to repression of biosynthesis in Arginine metabolism of Saccharomyces cerevisiae. J. Mol. Biol. 122, 383–406 (1978)
Douglas, H.C., Hawthorne, D.C.: Regulation of genes controlling synthesis of the galactose pathway enzymes in yeast. Genetics 54, 911–916 (1966)
Downie, J.A., Stewart, J.W., Brockman, M., Schweingruber, A.M., Sherman, F.: Structural gene for yeast iso-2 cytochrome c. J. Mol. Biol. 133, 369–384 (1977)
Hawthorne, D.: The selection of nonsense suppressors in yeast. Mutat. Res. 7, 187–197 (1969)
Hynes, M.: A Cis dominant regulatory mutation affecting enzyme induction in the eukaryote Aspergillus nidulans. Nature 253, 210–211 (1975)
Messenguy, F.: Regulation of arginine biosynthesis in Saccharomyces cerevisiae isolation of a cis-dominant, constitutive mutant for ornithine carbamoyltransferase synthesis. J. Bacteriol. 128, 49–55 (1976)
Péré-Aubert, G.: Aspects de la régulation de la biosynthèse du cytochrome c chez la levure. Thèse de Doctorat d'Etat (1970)
Petrochilo, E., Verdiére, J.: A glycine to serine substitution identifies the CYP3 locus as the structural gene of iso-2 cytochrome c in Saccharomyces cerevisiae. Biochem. Biophys. Res. Commun. 79, 364–271 (1977)
Sherman, F., Stewart, J.W., Helms, C., Downie, A.: Chromosome mapping of the CYC7 gene determining yeast iso-2 cytochrome c: Structural and regulatory regions. Proc. Natl. Acad. Sci. U.S.A. 75, 1437–1441 (1978)
Sherman, F., Stewart, J.W., Margoliash, E., Parker, J., Campbell, W.: The structural gene for yeast cytochrome c. Proc. Natl. Acad. Sci. U.S.A. 55, 1498–1504 (1966)
Thuriaux, P., Ramos, F., Pierard, A., Grenson, M., Wiame, J.M.: Regulation of the carbomoylphosphate synthetase belonging to the arginine biosynthetic pathway of Saccharomyces cerevisiae. J. Mol. Biol. 67, 277–287 (1972)
Verdière, J., Petrochilo, E.: CYP3, a likely candidate for the structural gene of iso-2 cytochrome c in Saccharomyces cerevisiae. Biochem. Biophys. Res. Commun. 67, 1451–1458 (1975)
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Communicated by W. Gajewski
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Verdière, J., Petrochilo, E. CYP3–15: An up-promoter mutation at the ISO-2 cytochrome c structural gene locus and its interaction with other independent regulatory mutations of cytochrome c synthesis in Saccharomyces cerevisiae . Molec. Gen. Genet. 175, 209–216 (1979). https://doi.org/10.1007/BF00425538
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DOI: https://doi.org/10.1007/BF00425538