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Effect of DNA sequences adjacent to the termini of Tn3 on sequential translocation

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Summary

Insertion of Tn3 generates a five base pair repeat of a nucleotide sequence indigenous to the recipient genome. Tn3 promoted deletions extend precisely from the Tn3 terminus and remove one of the 5 base pair repeats while not affecting the ability of Tn3 to subsequently undergo translocation. A direct repeat of a 10 bp sequence located in the Tn3 termini occurs internally within Tn3 and may affect the orientation of insertion.

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References

  • Allet, B.: Mu insertion duplicates a 5 base pair sequence at the host inserted site. Cell 16, 123–129 (1979)

    Google Scholar 

  • Berg, D., Davies, J., Allet, B., Rochaix, J.-D.: Transposition of R factor genes to bacteriophage lambda. Proc. Natl. Acad. Sci. U.S.A. 72, 3628–3632 (1975)

    Google Scholar 

  • Berg, D.E.: Insertion and excision of the transposable kanamycin resistance determinant Tn5. In: DNA insertion elements, plasmids and episomes (A.I. Bukhari, J.A. Shapiro, S. Adhya, eds.) pp. 205–218. Cold Spring Harbor, NY: Cold Spring Harbor Press 1977

    Google Scholar 

  • Botstein, D., Kleckner, N.: Translocation and illegitimate recombination by the tetracycline resistance element Tn10. In: DNA insertion elements, plasmids and episomes (A.I. Bukhari, J.A. Shapiro, S. Sdhya, eds.), pp. 185–203. Cold Spring Harbor NY: Cold Spring Harbor Press 1977

    Google Scholar 

  • Brevet, J., Kopecko, D.J., Nisen, P., Cohen, S.N.: Promotion of insertions and deletions by translocating segments of DNA carrying antibiotic resistance genes. In: DNA insertions elements, plasmids and episomes (A.I. Bukhari, J.A. Shapiro, S. Adhya, eds.), pp. 169–178. Cold Spring Harbor, NY: Cold Spring Harbor Press 1977

    Google Scholar 

  • Bukhari, A.I., Shapiro, J.A., Adhya, S.: DNA insertion elements, plasmids and episomes (A.I. Bukhari, J.A. Shapiro, S. Adhya, eds.), pp. 169–178. Cold Spring Harbor, NY: Cold Spring Harbor Press 1977

    Google Scholar 

  • Bukhari, A.I., Shapiro, J.A., Adhya, S.: DNA insertion elements, plasmids and episomes (A.I. Bukhari, J.A. Shapiro, S. Adhya, eds.). Cold Spring Harbor, NY: Cold Spring Harbor Press 1977

    Google Scholar 

  • Calos, M.P., Johnsrud, L., Miller, J.H.: DNA sequence at the integration sites of the insertion element IS1. Cell 13, 411–418 (1978)

    Google Scholar 

  • Chang, A.C.Y., Cohen, S.N.: Construction and characterization of amplifiable multi-copy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J. Bacteriol. 134, 1141–1156 (1978)

    Google Scholar 

  • Cohen, S.N.: Transposable genetic elements and plasmid evolution. Nature 263, 731–738 (1976)

    Google Scholar 

  • Cohen, S.N., Casadaban, M.J., Chou, J., Tu, C.-P.D.: Studies of the specificity and control of transposition of the Tn3 element. Cold Spring Harbor Symp. Quant. Biol. 43, 1247–1255 (1979)

    Google Scholar 

  • Foster, T.J., Willetts, N.S.: Genetic analysis of delections of R100-1 that are both transfer-deficient and tetracycline-sensitive. J. Gen. Microbiol. 93, 133–140 (1976)

    Google Scholar 

  • Ghosal, D., Sommer, H., Saedler, H.: Nucleotide sequence of the transposable element IS2. Nucleic Acid Res. 6, 1111–1122 (1979)

    Google Scholar 

  • Gottesman, M.M., Rosner, J.L.: The acquisition of a determinant for chloramphenicol resistance by coliphage lambda. Proc. Natl. Acad. Sci. U.S.A. 72, 5041–5045 (1975)

    Google Scholar 

  • Grindley, N.D.F.: IS1 insertion generates duplication of a nine base pair sequence at its target site. Cell 13, 419–426 (1978)

    Google Scholar 

  • Haberman, P., Klaer, R., Kuhn, S., Starlinger, P.: IS4 is found between eleven or twelve base pair duplications. Mol. Gen. Genet. 175, 369–373 (1979)

    Google Scholar 

  • Heffron, F., Rubens, C., Falkow, S.: Translocation of a plasmid DNA sequence which mediates ampicillin resistance: Molecular nature and specificity of insertion. Proc. Natl. Acad. Sci. U.S.A. 72, 3623–2627 (1975)

    Google Scholar 

  • Johnsrud, L., Calos, M.P., Miller, J.H.: The transposon Tn9 generates a 9 bp repeated sequence during integration. Cell 15, 1209–1219 (1978)

    Google Scholar 

  • Kleckner, N.: Translocatable elements in prokaryotes. Cell 11, 11–23 (1977)

    Google Scholar 

  • Kleckner, N.: DNA sequence analysis of Tn10 insertions: Origin and role of 9 bp flanking repetitions during Tn10 translocation. Cell 16, 711–720 (1979)

    Google Scholar 

  • Kleckner, N., Chan, R.K., Tye, B.-K., Botstein, D.: Mutagenesis by insertion of a drug-resistance element carrying an inverted repetition. J. Mol. Biol. 97, 561–575 (1975)

    Google Scholar 

  • Kopecko, D.J., Brevet, J., Cohen, S.N.: Involvement of multiple translocating DNA segments and recombinational hot spots in the structural evolution of bacterial plasmids. J. Mol. Biol. 108, 333–360 (1976)

    Google Scholar 

  • Kopecko, D.J., Cohen, S.N.: Site-specific recA-independent recombination between bacterial plasmids: Involvement of palindromes at the recombination loci. Proc. Natl. Acad. Sci. U.S.A. 72, 1373–1377 (1975)

    Google Scholar 

  • Lehman, I.R., Chien, J.R.: Persistence of deoxyribonucleic acid polymerase I and its 5′→3′ exonuclease activity in polA mutants of Escherichia coli K12. J. Biol. Chem. 248, 7717–7723 (1973)

    Google Scholar 

  • Maxam, A.M., Gilbert, W.: A new method for DNA sequence analysis. Proc. Natl. Acad. Sci. U.S.A. 74, 560–564 (1977)

    Google Scholar 

  • Monk, M., Kinross, J.: Conditional lethality of recA and recB derivatives of a strain of Escherichia coli K12 with a temperature-sensitive deoxyribonucleic acid polymerase I. J. Bacteriol. 109, 971–978 (1972)

    Google Scholar 

  • Nevers, P., Saedler, H.: Transposable genetic elements as agents of gene instability and chromosomal rearrangement. Nature 268, 109–115 (1977)

    Google Scholar 

  • Nisen, P.D., Kopecko, D.J., Chou, J., Cohen, S.N.: Site-specific DNA deletions occurring adjacent to the termini of a transposable ampicillin resistance element (Tn3). J. Mol. Biol. 117, 975–998 (1977)

    Google Scholar 

  • Ohtsubo, H., Ohmori, H., Ohtsubo, E.: Nucleotide sequence analysis of the ampicillin resistance transposon Tn3. Cold Spring Harbor Symp. Quant. Biol. 43, 1269–1277 (1979)

    Google Scholar 

  • Ohtsubo, H., Ohtsubo, E.: Nucleotide sequence of an insertion element, IS1. Proc. Natl. Acad. Sci. U.S.A. 75, 615–619 (1978)

    Google Scholar 

  • Oka, A., Nomura, N., Sugimoto, K., Sugimoto, K., Sugisaki, H., Takanami, M.: Nucleotide sequence at the insertion sites of a kanamycin transposon. Nature 276, 845–847 (1978)

    Google Scholar 

  • Peterson, P.A., Ghosal, D., Sommer, H., Saedler, H.: Development of a system useful for studying the formation of unstable alleles of IS2. Mol. Gen. Genet. 173, 15–22 (1979)

    Google Scholar 

  • Reif, H.J., Saedler, H.: IS1 in involved in deletion formation in the Gal region of E. coli K12. Mol. Gen. Genet. 131, 17–28 (1975)

    Google Scholar 

  • Rosenberg, M., Court, D., Shimatake, H., Brady, C., Wulff, D.L.: The relationship between function and DNA sequence in an inter-cistronic regulatory region in phage lambda. Nature 272, 414–422 (1978)

    Google Scholar 

  • Ross, D.G., Swan, J., Kleckner, N.: Physical structures of Tn10-promoted deletions and inversions: Role of 1400 bp inverted repetitions. Cell 16, 721–731 (1979)

    Google Scholar 

  • Roychoudhury, R., Jay, E., Wu, R.: Terminal labeling and addition of homopolymer tracts to duplex DNA fragments by terminal deoxynucleotidyl transferase. Nucleic Acid Res. 3, 863–878 (1976)

    Google Scholar 

  • Sanger, F., Air, G.M., Barrell, B.G., Brown, N.L., Coulson, A.R., Fiddes, J.C., Hutchison III, C.A., Slocombe, P.M., Smith, M.: Nucleotide sequence of bacteriophage ϕX174 DNA. Nature 265, 687–695 (1977)

    Google Scholar 

  • Starlinger, P., Saedler, H.: IS elements in microorganisms. Curr. Top. Microbiol. Immunol. 75, 111–152 (1976)

    Google Scholar 

  • Takeya, T., Nomiyama, H., Miyoshi, J., Shimada, K., Takagi, Y.: DNA sequence of the integration sites and inverted repeated structure of transposon Tn3. Nucleic Acid Res. 6, 1831–1841 (1979)

    Google Scholar 

  • Taylor, D.P., Cohen, S.N.: Molecular cloning of discrete DNA segments containing the replication and incompatibility regions of a miniplasmid derived from a copy mutant of NR1. J. Bacteriol. 137, 92–104 (1979)

    Google Scholar 

  • Timmis, K., Cabello, F., Cohen, S.N.: Cloning and characterization of EcoRI a and HindIII restriction endonuclease-generated fragments of antibiotic resistance plasmid R6-5 and R6. Mol. Gen. Genet. 162, 121–137 (1978)

    Google Scholar 

  • Tu, C.-P.D., Cohen, S.N.: Translocation specificity of the Tn3 element: Characterization of sites of multiple insertions. Cell 19, 151–160 (1980)

    Google Scholar 

  • Wu, R., Jay, E., Roychoudhury, R.: Nucleotide sequence analysis of DNA. Methods Cancer Res. 12, 87–176 (1976)

    Google Scholar 

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Authors and Affiliations

  1. Department of Genetics, Stanford University, School of Medicine, 94305, Stanford, California, USA

    Chen-Pei D. Tu & Stanley N. Cohen

  2. Department of Medicine, Stanford University, School of Medicine, 94305, Stanford, California, USA

    Chen-Pei D. Tu & Stanley N. Cohen

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  1. Chen-Pei D. Tu
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  2. Stanley N. Cohen
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Communicated by J. Schell

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Tu, CP.D., Cohen, S.N. Effect of DNA sequences adjacent to the termini of Tn3 on sequential translocation. Molec. Gen. Genet. 177, 597–601 (1980). https://doi.org/10.1007/BF00272669

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  • DOI: https://doi.org/10.1007/BF00272669

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