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Identification and characterization of a locus inhibiting extrachromosomal maintenance of theStreptomyces plasmid SLP1

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Summary

We report here the existence and initial characterization of a genetic locus (imp) that inhibits maintenance of SLP1-derived plasmids as extrachromosomal replicons in a manner distinct from normal incompatibility between autonomous SLP1 replicons. Thetrans-actingimp function has been localized to a 1.8 kbEco47III restriction fragment present on integrated SLP1 elements. At least part of this DNA segment is absent from SLP1-derived plasmids. DNA sequence analysis of theimp region indicates that it contains three overlapping open reading frames (ORFs) that may constitute a polycistronic operon. The effects of insertions within theimp region indicate that uninterrupted transcription through all three ORFs is necessary forimp activity.

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Author notes

  1. Stephen C. Lee

    Present address: Department of Genetics, Stanford University School of Medicine, 94305, Stanford, CA, USA

  2. Sarah R. Grant

    Present address: Max Planck Institut für Züchtungsforschung, D-5000, Köln 30, Federal Republic of Germany

Authors and Affiliations

  1. Department of Genetics, Stanford University School of Medicine, 94305, Stanford, CA, USA

    Sarah R. Grant, Kevin Kendall & Stanley N. Cohen

  2. Department of Medicine, Stanford University School of Medicine, 94305, Stanford, CA, USA

    Stanley N. Cohen

Authors
  1. Sarah R. Grant
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  2. Stephen C. Lee
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  3. Kevin Kendall
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  4. Stanley N. Cohen
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Communicated by W. Goebel

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Grant, S.R., Lee, S.C., Kendall, K. et al. Identification and characterization of a locus inhibiting extrachromosomal maintenance of theStreptomyces plasmid SLP1. Molec. Gen. Genet. 217, 324–331 (1989). https://doi.org/10.1007/BF02464900

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  • DOI: https://doi.org/10.1007/BF02464900

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