Summary
A transformation system for the thermophilic cellulolytic fungus Talaromyces sp. CL240 has been developed, using the phleomycin resistance gene from Streptoalloteichus hindustanus (Sh ble) as a dominant selectable marker. The plasmids (pAN8-1 and pUT720) carrying the Sh ble gene under the control of the Aspergillus nidulans glyceraldehyde-3-phosphate dehydrogenase (gpd) promoter, allowed selection of phleomycin-resistant transformants. A new promoter sequence cloned from chromosomal DNA of Trichoderma reesei (pUT737) was also able to drive efficient expression of the Sh ble gene in Talaromyces sp. CL240, resulting in the selection of transformants that were highly resistant to phleomycin.
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Armau E, Droucourt D, Etienne G, Tiraby G (1984) Application des antibiotiques de la famille des phléomycines à titre d'agent de sélection dans le domaine du génie génétique. European Patent No. 8413502
Ballance DJ, Buxton FP, Turner G (1983) Transformation of Aspergillus nidulans by the orotidine-5-phosphate decarboxylase gene of Neurospora crassa. Biochem Biophys Res Commun 112:284–289
Banks GR (1983) Transformation of Ustilago maydis by a plasmid containing yeast 2-micron DNA. Curr Genet 7:73–77
Bej AK, Perlin MH (1989) A high efficiency transformation system for the basidiomycete Ustilago violacea employing hygromycin resistance and lithium acetate treatment. Gene 80:171–176
Berdy J (1980) Bleomycin type antibiotics. In: Handbook of antibiotic compounds, vol IV. CRC Press, Boca Raton, pp 459–491
Birnboim HC, Doly J (1979) A rapid alkaline procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7:1513–1523
Bull JH, Smith DJ, Turner G (1988) Transformation of Penicillium chrysogenum with a dominant selectable marker. Curr Genet 13:377–382
Calmels TPG, Martin F, Durand H, Tiraby G (1991) Proteolytic events in the processing of secreted proteins in fungi. J Biotech 17:51–66
Case ME, Schweizer M, Kushner SR, Giles NH (1979) Efficient transformation of Neurospora crassa by utilizing hybrid plasmid DNA. Proc Natl Acad Sci USA 76:5259–5263
Collins CM, Hall RH (1985) Identification of a Tn5 determinant conferring resistance to phleomycins, bleomycins and tallysomycins. Plasmid 14:143–151
Droucourt D, Calmels T, Reynes JP, Baron M, Tiraby G (1990) Cassettes of the Streptoalloteichus hindustanus ble gene for transformation of lower and higher eukaryotes to phleomycin resistance. Nucleic Acids Res 18:4009
Durand H, Baron M, Calmels T, Tiraby G (1988) Classical and molecular genetics applied to Trichoderma reesei for the selection of improved cellulolytic industrial strains. In: Aubert JP, Beguin P, Millet J (eds) Biochemistry and genetics of cellulose degradation. Academic Press, London, pp 135–151
Hanahan D (1985) Techniques for transformation of E. coli. In: Glover DM (ed) DNA cloning — a practical approach, vol I. IRL Press, Oxford, pp 109–135
Jain S, Tiraby G (1987) Separation and characterization of the cellulolytic components of a thermophilic fungus, Talaromyces sp. CL240. In: Grass G, Delmon B, Holle JI, Zibetta H (eds) Biomass for energy and industry. Elsevier Science Publishers, England, pp 355–359
Kolar M, Punt PJ, Van den Hondel CAMJJ, Schwab H (1988) Transformation of Penicillium chrysogenum using dominant selection markers and expression of an E. coli lacZ fusion gene. Gene 62:127–134
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurements with the folin phenol reagent. J Biol Chem 193:265–275
Mandels M, Weber J (1969) The production of cellulases. Adv Chem Ser 95:391–414
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Mattern JE, Punt PJ (1988) A vector of Aspergillus transformation conferring phleomycin resistance. Fungal Genet Newslett 35:25
Osiewacz HD, Weber A (1989) DNA mediated transformation of the filamentous fungus Curvularia lunata using a dominant selectable marker. Appl Microbiol Biotechnol 30:375–380
Picknett TM, Saunders G (1989) Transformation of Penicillium chrysogenum with selection for increased resistance to benomyl. FEMS Microbiol Lett 60:165–168
Punt PJ, Oliver RP, Dingemanse MA, Pouwels PH, Van den Hondel CAMJJ (1987) Transformation of Aspergillus based on the hygromycin B resistance marker from E. coli. Gene 56:117–124
Raeder U, Broda P (1985) Rapid preparation of DNA from filamentous fungi. Lett Appl Microbiol 1:17–20
Semon D, Movva NR, Smith TF, El Alama M, Davies J (1987) Plasmid-determined bleomycin resistance in Staphylococcus aureus. Plasmid 17:46–53
Skatrud PL, Queener SW, Carr LG, Fisher DL (1987) Efficient integrative transformation of Cephalosporium acremonium. Curr Genet 12:337–348
Soliday CL, Dickman MB, Kolattukudy PE (1989) Structure of cutinase gene and detection of promoter activity in the 5′-flanking region by fungal transformation. J Bacteriol 171:1942–1951
Stahl U, Leitner E, Esser K (1987) Transformation of Penicillium chrysogenum by a vector containing a mitochondrial origin of replication. Appl Microbiol Biotechnol 26:237–241
Tilburn J, Scazzochio C, Taylor GG, Zabicky-Zissima JH, Lockington RA, Davis RW (1983) Transformation by integration in Aspergillus nidulans. Gene 26:205–221
Umezawa H (1976) Structure and action of bleomycin. Prog Biochem Pharmacol 11:18–27
Van Engelenburg F, Smith R, Goosen T, Van den Broek M, Tudzynski P (1989) Transformation of Claviceps purpurea using a bleomycin resistance gene. Appl Microbiol Biotechnol 30:364–370
Vollmer SJ, Yanofsky C (1986) Efficient cloning of genes of Neurospora crassa. Proc Natl Acad Sci USA 83:4867–4873
Ward M, Wilkinson B, Turner G (1986) Transformation of Aspergillus nidulans with a cloned oligomycin-resistant ATP synthase subunit 9 gene. Mol Gen Genet 202:265–270
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Jain, S., Durand, H. & Tiraby, G. Development of a transformation system for the thermophilic fungus Talaromyces sp. CL240 based on the use of phleomycin resistance as a dominant selectable marker. Molec. Gen. Genet. 234, 489–493 (1992). https://doi.org/10.1007/BF00538710
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DOI: https://doi.org/10.1007/BF00538710