Abstract
The imperfect yeast Candida maltosa has an ill-defined genetic constitution; it is nominally diploid, but probably highly aneuploid, in nature. We report on polymorphisms specifically affecting those chromosomes which bear the cm-ADE1 gene. This gene encodes phosphoribosylaminoimidazole-succinocarboxamide synthetase, an enzyme in the adenine biosynthetic pathway. By electrophoretic karyotype analysis, three differently sized chromosomes were demonstrated to carry cm-ADE; the size (but not the number) of these chromosomes was also found to vary, both between strains and during the mitotic growth of a single strain. Four different alleles of cm-ADE1 have been cloned and sequenced from one prototrophic strain. DNA sequence divergence between these different alleles is as high as 8%, with the greatest divergence being found in the upstream region. Mitotic recombination events that led to changes in the karyotype were followed by using cm-ADE1 DNA as an hybridization probe. A recombination hot-spot in the neighbourhood of the gene appears to be responsible for the instability of the chromosomes on which it resides.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Akins RA, Lambowitz AM (1985) General method for cloning Neurospora crassa nuclear genes by complementation of mutants. Mol Cell Biol 5:2272–2278
Becher D, Wedler H, Schulze S, Bode R, Kasuske A, Samsonova I (1991) Correlation of biochemical blocks and genetic lesions in leucine auxotrophic strains of the imperfect yeast Candida maltosa. Mol Gen Genet 227:361–368
Becher D, Schulze S, Kasuske A, Wedler H, Samsonova I, Oliver SG (1994) Molecular analysis of a leu2 mutant of Candida maltosa demonstrates presence of multiple alleles. Curr Genet 26:208–216
Becher-André M, Hahlbrock K (1989) Absolute mRNA quantification using the polymerase chain reaction (PCR). A novel approach by PCR-aided transcript titration assay (PATTY). Nucleic Acids Res 17:9437–9446
Boeke JD, Sandmeyer SB (1991) Yeast transposable elements. In: Broach JR, Pringle JR, Jones EW (eds) The molecular and cellular biology of the yeast Saccharomyces. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 193–261
Brooks P, Sims P Broda P (1993) Isozyme-specific polymerase chain reaction analysis of differential gene expression: a general method applied to lignin petroxidase genes of Phanerochaete chrysosporium. Bio/Technology 11:830–834
Carle GF, Olson MV (1984) Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis. Nucleic Acids Res 12:5647–5664
Clarke L, Carbon J (1976) A colony bank containing synthetic ColE1 hybrid plasmids representative of the entire E. coli genome. Cell 9:91–99
Coleman KG, Steensma HY, Kaback, DB, Pringle JR (1986) Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: isolation and characterization of the CDC24 gene and adjacent regions of the chromosome. Mol Cell Biol 6:4516–4525
Cryer DR, Eccleshall R, Marmur J (1975) Isolation of yeast DNA. Methods Cell Biol 12:41–45
Dujon B (1989) Group I introns as mobile genetic elements: facts and mechanistic speculations — a review. Gene 82:91–114
Feinberg AP, Vogelstein B (1983) A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13
Foury F, Lahaye A (1987) Cloning and sequencing of the PIF gene involved in repair and recombination of yeast mitochondrial DNA. EMBO J 6:1441–1449
Higgins MJ, Turmel C, Noolandi J, Lalande M (1989) Detection of Saccharomyces cerevisiae size markers directly on Southern blots of pulsed-field gels with a single hybridization probe. Nucleic Acids Res 17:10136
Ito H, Fukuda, Y, Muruda K, Kimura A (1983) Transformation of intact yeast cells treated with alkali cations. J Bacrteriol 153:163–168
Kamiryo T, Mito N, Niki T, Suzuki T (1991) Assignment of most genes encoding major peroxisomal polypeptides to chromosomal band V of the asporogenic yeast Candida tropicalis. Yeast 7:503–511
Kasüske A, Wedler H, Schulze S, Becher D (1992) Efficient electropulse transformation of intact Candida maltosa cells by different homologous vector plasmids. Yeast 8:691–697
Kawai S, Hikiji T, Murao S, Takagi M, Yano K (1991) Isolation and sequencing of a gene, C-ADEI, and its use for a host-vector system in Candida maltosa with two genetic markers. Agric Biol Chem 55:59–65
Kawamura M, Takagi M, Yano K (1982) Cloning of a LEU gene and an ARS site of Candida maltosa. Gene 24:157–162
Lott TJ, Kuykendall RJ, Welbel SF, Pramanik A, Lasker BA (1993) Genomic heterogeneity in the yeast Candida parapsilosis. Curr Genet 23:463–467
Maniatis T, Fritsch EF, Sambroook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
1Mortimer RK, Schild D, Contopoulo CR, Kans JA (1989) Genetic map of Saccharomyces cerevisiae, edition 10. Yeast 5:321–404
O'Connell MP (1984) Genetic transfer in prokaryotes: transformation, transduction, and conjugation. In: Pühler A, Timmis KN (eds) Advanced molecular genetics. Springer, Berlin, pp 2–5
Oliver SG et al. (1992) The complete DNA sequence of yeast chromosome III. Nature 357:38–46
Ono B, Ishino-Aran Y (1988) Inheritance of chromosome length polymorphisms in Saccharomyces cerevisiae. Curr Genet 14:413–418
Prentki P, Karch F, Iida S, Meyer J (1981) The plasmid cloning vector pBR325 contains a 482 base-pair-long inverted duplication. Gene 14:289–299
Rustchenko-Bulgac EP, Sherman F, Hicks JB (1990) Chromosomal rearrangements associated with morphological mutants provide a means for genetic variation of Candida albicans. J Bacteriol 172:1276–1283
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467
Sasnauskas K, Jomantiene R, Geneviciute E, Januska A, Lebedys J (1991) Molecular cloning of the Candida maltosa ADE1 gene. Gene 107:161–164
Sasnauskas K, Jomantiene R, Januska A, Lebidiene E, Lebedys J, Janululaitis A (1992) Cloning and analysis of a Candida maltosa gene which confers resistance to formaldehyde in Saccharomyces cerevisiae. Gene 82:91–114
Schrank A, Tempelaars C, Sims PFG, Oliver SG, Broda P (1991) The trpC gene of Phanerochaete chrysosporium is unique in containing an intron but nevertheless maintains the order of functional domains found in other fungi. Mol Microbiol 2:467–476
Schwartz DC, Cantor CR (1984) Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell 37:67–75
Szostak JW, Wu R (1980) Unequal crossing over in the ribosomal DNA of Saccharomyces cerevisiae. Nature 284:426–430
Wedler H (1990) Moleculargenetische Charakterisierung der Hefe Candida maltosa L4. PhD Thesis, Ernst-Moritz-Arndt University, Greifswald, Germany
Wicksteed BL, Collins I, Dershowitz A, Stateva LI, Green RP, Oliver SG, Brown AJP, Newlon CS (1994) A physical comparison of chromosome III in six strains of Saccharomyces cerevisiae. Yeast 10:39–57
Wolf K (1994) Mitochondrial introns in yeast — mobile genetic elements. Endocytobiosis Cell Res 10:55–63
Yanisch-Perron C, Vieira J, Messing J (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103–119
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Becher, D., Schulze, S., Kasüske, A. et al. Chromosome polymorphisms close to the cm-ADE1 locus of Candida maltosa . Molec. Gen. Genet. 247, 591–602 (1995). https://doi.org/10.1007/BF00290351
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00290351