Abstract
The replication behaviour of a number ofARS1-based plasmids was investigated on propagation inSaccharomyces cerevisiae grown with either glucose or galactose as carbon source. Growth on galactose results in reduced plasmid stability, as well as in reduced replication efficiency, when the entire 1.5-kbTRP1-ARS1 fragment is present on a plasmid. The galactose sensitivity is mediated by a 0.13-kb fragment harbouring part of theGAL3 promoter. This fragment exerts its effect when situated either 5′ or 3′ to the ARS core consensus at distances up to 0.9 kb. The endogenous 2 µm plasmid remained unaffected by the choice of carbon source.
References
Bajwa W, Torchia TE, Hopper JE (1988) Yeast regulatory geneGAL3; UASGal elements in common withGAL1, GAL2, GAL7, GAL10, GAL80, andMEL1; encoded protein strikingly similar to yeast andEscherichia coli galactokinase. Mol Cell Biol 8:3439–3447
Gietz D, Jean AS, Woods RA, Schiestl RH (1992) Improved method for high efficiency transformation of intact yeast cells. Nucleic Acids Res 20:1425
Gulløv K, Friis J (1985) Maintenance and copy number control ofARS1 plasmids inSaccharomyces cerevisiae. Curr Genet 10:21–27
Johnston M, Carlson M (1992) Regulation of carbon and phosphate utilization. In: Jones EW, Pringle JR, Broach JR (eds) The molecular and cellular biology of the yeastSaccharomyces vol 2. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, pp 193–282
Kirpekar F, Gulløv K (1992) Mutational analysis of a variant ofARS1 fromSaccharomyces cerevisiae. Curr Genet 22:175–180
Kirpekar F, Friis J, Gulløv K (1994) A search for an essential function of the replication originARS1 in the life cycle ofSaccharomyces cerevisiae. Yeast 10:491–496
Mahrahrens Y, Stillman B (1992) A yeast chromosomal origin of replication defined by multiple functional elements. Science 255:817–823
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
Snyder M, Buchman AR, Davis RW (1986) Bent DNA at a yeast autonomously replicating sequence. Nature 324:87–89
Snyder M, Sapolsky RJ, Davis RW (1988) Transcription interferes with elements important for chromosome maintenance inSaccharomyces cerevisiae. Mol Cell Biol 8:2184–2194
Tschumper G, Carbon J (1980) Sequence of a yeast DNA fragment containing a chromosomal replicator and theTRP1 gene. Gene 10:157–166
Venditti P, Costanzo G, Negri R, Camilloni G (1994) ABF1 contributes to the chromatin organization ofSaccharomyces cerevisiae ARS1 B-domain. Biochim Biophys Acta 1219:677–689
Zakian VA, Scott JF (1982) Construction, replication, and chromatin structure of TRP1 RI circle, a multiple-copy synthetic plasmid derived fromSaccharomyces cerevisiae chromosomal DNA. Mol Cell Biol 3:221–232
Author information
Authors and Affiliations
Additional information
Communicated by K. Isono
Rights and permissions
About this article
Cite this article
Kirpekar, F., Gulløv, K. Replication properties ofARS1 plasmids inSaccharomyces cerevisiae: dependence on the carbon source. Molec. Gen. Genet. 251, 716–719 (1996). https://doi.org/10.1007/BF02174121
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02174121