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Isoelectric focusing with immobilized pH gradients for the analysis of transferrin (Tf) subtypes and variants

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Summary

The human serum transferrin (Tf) system was analyzed by isoelectric focusing (IEF) with immobilized pH gradients. For the demonstration of the genetic variability the Fe1-Tf region was chosen. The pH range suitable for analysis of the Tf system was pH 5.20 to pH 5.75. The phenotypes of the common six TfC subtypes are described. No further heterogeneity among TfC1, TfC2, and TfC3 was noted. Also presented are the phenotypes of the TfC6 subtype, and of three different TfB and three different TfD variants. IEF with immobilized pH gradients appears to be a suitable method for the analysis of the inherited transferrin polymorphism.

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Authors and Affiliations

  1. Lehrstuhl für Allgemeine Lebensmitteltechnologie, Technische Universität München, D-8050, Freising-Weihenstephan, Federal Republic of Germany

    Angelika Görg, J. Weser, R. Westermeier & W. Postel

  2. Institut für Anthropologie und Humangenetik der Universität München, Richard-Wagner-Str. 10/I, D-8000, München 2, Federal Republic of Germany

    S. Weidinger, W. Patutschnick & H. Cleve

Authors
  1. Angelika Görg
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  2. J. Weser
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  3. R. Westermeier
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  4. W. Postel
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  5. S. Weidinger
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  6. W. Patutschnick
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  7. H. Cleve
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Görg, A., Weser, J., Westermeier, R. et al. Isoelectric focusing with immobilized pH gradients for the analysis of transferrin (Tf) subtypes and variants. Hum Genet 64, 222–226 (1983). https://doi.org/10.1007/BF00279397

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  • DOI: https://doi.org/10.1007/BF00279397

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