Summary
We have studied the genetic linkage relationships of seven DNA polymorphisms on chromosome 19, with each other and with the myotonic dystrophy locus. The DNA sequences were localised to various regions of the chromosome using translocations in somatic cell hybrids. These results provide the basis for a linkage map of most of chromosome 19, and suggest that the myotonic dystrophy locus is close to the centromere.
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Berg K, Borresen AL, Heiberg A, Maartmann-Moe K (1984) Study of the linkage relationships between ApoE and familial hypercholesterolemia with xanthomatosis. Cytogenet Cell Genet 37: 418
Brook JD, Shaw DJ, Meredith AL, Bruns GAP, Harper PS (1984) Localisation of genetic markers and orientation of the linkage group on chromosome 19. Hum Genet 68:282–285
Brook JD, Shaw DJ, Meredith AL, Worwood M, Cowell J, Scott J, Knott TJ, Litt M, Bufton L, Harper PS (1985) A somatic cell hybrid panel for chromosome 19: localisation of known genes and RFLPs and orientation of the linkage group. Cytogenet Cell Genet 40:590–591
Brook JD, Shaw DJ, Thomas NST, Meredith AL, Cowell J, Harper PS (1986) Mapping genetic markers on human chromosome 19 using subchromosomal fragments in somatic cell hybrids. Cytogenet Cell Genet 41:30–37
Bufton L, Bruns G, Magenis RE, Tomar D, Shaw D, Brook D, Litt M (1985) Four RFLPs revealed by probes from a single cosmid map to chromosome 19. Cytogenet Cell Genet 40:596
Davies KE, Jackson J, Williamson R, Harper PS, Ball S, Sarfarazi M, Meredith L, Fey G (1983) Linkage analysis of myotonic dystrophy and sequences on chromosome 19 using a cloned complement 3 gene probe. J Med Genet 20:259–263
Donald JA, Wallis SC, Kessling A, Tippett P, Robson EB, Ball S, Davies KE, Scambler P, Berg K, Heiberg A, Williamson R, Humphries SE (1985) Linkage relationships of the gene for apolipoprotein CH with loci on chromosome 19. Hum Genet 69:39–43
Hulsebos T, Brunner H, Wieringa B, Friedrich U, Smeets D, Oci T, Hustinx T, Scheres J, Wienker T, Humphries S, Myklebost O, Junien C, ter Haar B, Ropers HH (1985) Regional assignment of C3, GPI, APOC2, and BHCG and their linkage relationships with DM and 19cen. Cytogenet Cell Genet 40:658
Humphries SE, Horsthemke B, Seed M, Holm M, Wynn V, Kessling AM, Donald JA, Jowett N, Galton DJ, Williamson R (1985) A common DNA polymorphism of the low-density lipoprotein (LDL) receptor gene and its use in diagnosis. Lancet I:1003–1005
Lathrop GM, Lalouel JM, Julier C, Ott J (1984) Strategies for multilocus linkage analysis in humans. Proc Natl Acad Sci USA 81: 3443–3446
Lindgren V, Luskey KL, Russell DW, Franke U (1985) Human genes involved in cholesterol metabolism: chromosomal mapping of the loci for the low-density lipoprotein receptor and 3-hydroxy-3-methylglutaryl coenzyme A reductase with cDNA probes. Proc Natl Acad Sci USA 82:8567–8571
Lusis AJ, Heinzmann C, Sparkes RS, Geller R, Sparkes MC, Mohandas T (1985) Regional mapping on human chromosome 19: apolipoprotein E, apolipoprotein CII, low density lipoprotein (LDL) receptor, peptidase D, glucose phosphate isomerase. Cytogenet Cell Genet 40:683
Myklebost O, Williamson R, Markham AF, Myklebost SR, Rogers J, Woods DE, Humphries SE (1984) The isolation and characterisation of cDNA clones for human apolipoprotein CII. J Biol Chem 259:4401–4404
O'Brien T, Ball S, Sarfarazi M, Harper PS, Robson EB (1983) Genetic linkage between the loci for myotonic dystrophy and peptidase D. Ann Hum Genet 47:117–121
Ott J (1974) Estimation of the recombination fraction in human pedigrees: efficient computation of the likelihood for human linkage studies. Am J Hum Genet 26:588–597
Shaw DJ, Meredith AL, Sarfarazi M, Huson SM, Brook JD, Myklebost O, Harper PS (1985) The apolipoprotein CII gene: subchromosomal localisation and linkage to the myotonic dystrophy locus. Hum Genet 70:271–273
Sherman SL, Ball SP, Robson EB (1985) A genetic map of chromosome 19 based on family linkage data. Ann Hum Genet 49:181–187
Whitehead AS, Solomon E, Chambers S, Bodmer WF, Povey S, Fey G (1982) Assignment of the structural gene for the third component of human complement to chromosome 19. Proc Natl Acad Sci USA 79:5021–5025
Worwood M, Brook JD, Cragg SJ, Hellkuhl B, Jones BM, Perera P, Roberts SH, Shaw DJ (1985) Assignment of human ferritin genes to chromosomes 11 and 19q13.3-19qter. Hum Genet 69:371–374
Yamamoto T, Davis CG, Brown MS, Schneider WJ, Casey ML, Goldstein JL, Russell DW (1984) The human LDL receptor: a cysteine-rich protein with multiple Alu sequences in its mRNA. Cell 39:27–38
Yang-Feng TL, Francke U, Ullrich A (1985) Gene for human insulin receptor: localisation to site on chromosome 19 involved in pre-B-cell leukemia. Science 228:728–731
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Shaw, D.J., Meredith, A.L., Sarfarazi, M. et al. Regional localisations and linkage relationships of seven RFLPs and myotonic dystrophy on chromosome 19. Hum Genet 74, 262–266 (1986). https://doi.org/10.1007/BF00282545
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DOI: https://doi.org/10.1007/BF00282545