Summary
The gene encoding human 17beta-hydroxy-steroid dehydrogenase (17-HSD; EC 1.1.1.62) is assigned to chromosome 17 by Southern blotting analyses of panels of human x rodent somatic cell hybrids and independently to 17q12–q21 using chromosomal in situ hybridization. A search for physical linkage between 17-HSD and the proto-oncogenes, THRA1 and ERBB2 (both reported to be located in this region of chromosome 17) was performed by pulsed-field gel electrophoresis (PFGE) using several rare-cutting restriction endonucleases. Because all three genes hybridized to DNA fragments of different lengths it seems unlikely that the gene for 17-HSD is located very close to THRA1 and ERBB2. Further evidence for this assumption was obtained from the absence of any coamplification of the 17-HSD gene in 9 breast tumors with amplification of the ERBB2 gene. Analyses of Southern blots of ScaI-digested DNAs from unrelated individuals from Northern Finland revealed a relatively infrequent diallelic restriction fragment length polymorphism, the allele frequencies of which were 0.04 (A1) and 0.96 (A2).
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Balazs J, Purello M, Kurnit DM, Grzeschik K-H, Siniscalco M (1984) Isolation and characterization of human random cDNA clones homologous to DNA from the X chromosome. Somat Cell Mol Genet 10:385–397
Bargmann CI, Hung M-C, Weinberg RA (1986) The neu oncogene encodes an epidermal growth factor receptor-related protein. Nature 319:226–230
Bonney RC, Reed MJ, Beranek PA, Ghilchik MW, James VHT (1986) Metabolism of 3H-oestradiol in vivo by normal breast and tumour tissue in postmenopausal women. J Steroid Biochem 24:361–364
Camerino G, Grzeschik K-H, Jaye M, Salle H de la, Tolstoshev P, Lecocq JP, Heilig R, Mandel J-L (1984) Regional localization on the human X chromosome and polymorphism of the coagulation factor IX gene (hemophilia B locus). Proc Natl Acad Sci USA 81:498–502
Coussens L, Yang-Feng TL, Liao Y-C, Chen E, Gray A, McGrath J, Seeburg PH, Libermann TA, Schlessinger J, Francke U, Levinson A, Ullrich A (1985) Tyrosine kinase receptor with extensive homology to EGF receptor shares chromosomal location with neu oncogene. Science 230:1132–1139
Feinberg AP, Vogelstein B (1984) A technique for radiolabeling DNA restriction endonuclease fragments to a high specific activity. Anal Biochem 137:266–267
Harper ME, Saunders GF (1981) Localization of single-copy DNA sequences on G-banded human chromosomes by in situ hybridization. Chromosoma 83:431–439
Human Gene Mapping 10 (1989) 10th International Workshop on Human Gene Mapping. Cytogenet Cell Genet 51:325
Liu H-C, Tseng L (1979) Estradiol metabolism in isolated human endometrial epithelial glands and stromal cells. Endocrinology 104:1674–1681
Mäentausta O, Peltoketo H, Isomaa V, Jouppila P, Vihko R (1990) Immunological measurement of human 17β-hydroxysteroid dehydrogenase. J Steroid Biochem (in press)
Mehta RR, Valcourt L, Graves J, Green R, Das Gupta TK (1987) Subcellular concentrations of estrone, estradiol, androstenedione and 17beta-hydroxysteroid dehydrogenase (17-beta-OH-SDH) activity in malignant and non-malignant human breast tissues. Int J Cancer 40:305–308
Peltoketo H, Isomaa V, Mäentausta O, Vihko R (1988) Complete amino acid sequence of human placental 17beta-hydroxysteroid dehydrogenase deduced from cDNA. FEBS Lett 239:73–77
Poutanen M, Isomaa V, Kainulainen K, Vihko R (1990) Progestin induction of 17β-hydroxysteroid dehydrogenase enzyme protein in the T-47D human breast cancer cell line. Int J Cancer (in press)
Prudhomme JF, Malet C, Gompel A, Lalardrie JP, Ochoa C, Boué A, Mauvais-Jarvis P, Kuttenn F (1984) 17Beta-hydroxysteriod dehydrogenase activity in human breast epithelial cell and fibroblast cultures. Endocrinology 114:1483–1489
Sap J, Munoz A, Damm K, Goldberg Y, Ghysdael J, Leutz A, Berg H, Vennström B (1986) The c-erb-A protein is a high-affinity receptor for thyroid hormone. Nature 324:635–640
Satyaswaroop PG, Wartell DJ, Mortel R (1982) Distribution of progesterone receptor, estradiol dehydrogenase, and 20alpha-dihydroprogesterone dehydrogenase activities in human endometrial glands in vitro is restricted to the glandular epithelium. Endocrinology 111:743–749
Schwartz DC, Cantor CR (1984) Separation of yeast chromosome-sized DNAs by pulsed-field gradient gel electrophoresis. Cell 37:67–75
Shiloh Y, Shipley J, Brodeur GM, Bruns G, Korf B, Donlon T, Schreck RR, Seeger R, Sakai K, Latt SA (1985) Differential amplification, assembly, and relocation of multiple DNA sequences in human neuroblastomas and neuroblastoma cell lines. Proc Natl Acad Sci USA 82:3761–3765
Slamon DJ, Godolphin W, Jones LA, Holt JA, Wong SG, Keith DE, Levin WJ, Stuart SG, Udove J, Ullrich A, Press MF (1989) Studies of the HER-2/neu proto-oncogene in human breast and ovarian cancer. Science 244:707–712
Spurr NK, Solomon E, Jansson M, Sheer D, Goodfellow PN, Bodmer WF, Vennström B (1984) Chromosomal localization of the human homologues to erbA and B. EMBO J 3:159–163
Tseng L (1980) Hormonal regulation of steroid metabolic enzymes in human endometrium. In: Thomas JA, Singhal RL (eds) Advances in steroid hormone research, vol 4. Urban & Schwarzenberg, Baltimore München Wien, pp 329–361
Vandenplas S, Wiid I, Grobler-Rabie A, Brebner K, Ricketts M, Wallis G, Bester A, Boyd C, Mathew C (1984) Blot hybridization of genomic DNA. J Med Genet 21:164–172
Zabel BV, Naylor SL, Grzeschik K-H, Sakaguchi AY (1984) Regional assignment of human proto-oncogene c-myb to 6q21-qter. Somat Cell Mol Genet 10:105–108
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Winqvist, R., Peltoketo, H., Isomaa, V. et al. The gene for 17beta-hydroxysteroid dehydrogenase maps to human chromosome 17, bands q12–q21, and shows an RFLP with ScaI. Hum Genet 85, 473–476 (1990). https://doi.org/10.1007/BF00194219
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DOI: https://doi.org/10.1007/BF00194219