Summary
The human RAB genes share structural and biochemical properties with the RAS gene superfamily. The encoded RAB proteins show 38 to 75% amino acid identity with the yeast YPT1 and SEC4 gene products. We used four human RAB-cDNAs, RAB3B, RAB4, RAB5 and RAB6, to map the corresponding genes on human chromosomes. These genes were assigned to 1p32-p31, 1q42-q43, 3p24-p22 and 2q14-q21, respectively, by in situ hybridization.
Similar content being viewed by others
References
Balch WE (1989) Biochemistry of interorganelle transport. A new frontier in enzymology emerges from versatile in vitro model systems. J Biol Chem 264:16965–16968.
Cannizzaro LA, Emanuel BS (1984) An improved method for G-banding chromosomes after in situ hybridization. Cytogenet Cell Genet 38:308–311.
Cannizzaro LA, Madaule P, Hecht F, Axel R, Croce MC, Huebner K (1990) Chromosome localization of human ARH genes, a ras-related gene family. Genomics 6:197–203.
Casey PJ, Gilman AG (1988) G-protein involvement in receptor-effector coupling. J Biol Chem 263:2577–2580.
Caubet JF, Mathieu-Mahul D, Bernheim A, Larsen CJ, Berger R (1985) Human proto-oncogene c-mos maps to 8q11. EMBO J 4:2245–2248.
Chardin P, Tavitian A (1986) The “RAL” gene: a new “RAS” related gene isolated by the use of a synthetic probe. EMBO J 5:2203–2208.
Emanuel BS, Cannizzaro LA, Seyer JM, Myers JC (1985) Human alpha-1 (III) and alpha-2 (V) procollagen genes are located on the long arm of chromosome 2. Proc Natl Acad Sci USA 82:3385–3389.
Feinberg AP, Vogelstein B (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13.
Goud B, Salminen A, Walworth N, Novick P (1988) A GTP-binding protein required for secretion rapidly associates with secretory vesicles and the plasma membrane in yeast. Cell 53:753–768.
Haluska FG, Huebner K, Isobe M, Nishimura T, Croce CM, Vogt PK (1988) Localization of the human JUN protooncogene to chromosome region 1p31–32. Proc Natl Acad Sci USA 85:2215–2218.
Harper ME, Saunders GF (1981) Localization of single copy DNA sequences on G-banded human chromosomes by in situ hybridization. Chromosoma 83:431–439.
Huerre-Jeanpierre C, Henry I, Bernard M, Gallano P, Weil D, Grzeschik KH, Ramirez F, Junien C (1986) The pro-alpha-2 (V) collagen gene (COL5A2) maps to 2q14→2q32, syntenic to the pro-alpha-1 (III) collagen locus (COL3A1). Hum Genet 73:64–67.
Lowe DG, Capon DJ, Delwart E, Sakaguchi AY, Naylor SL, Goedel DV (1987) Structure of the human and murine R-ras genes, novel genes closely related to ras protooncogenes. Cell 48:137–146.
Madaule P, Axel R, Myers A (1987) Characterization of two members of the RHO gene family from the yeast saccharomyces cerevisiae. Proc Natl Acad Sci USA 84:779–783.
McBride OW, Kirsch I, Hollis G, Nau M, Battey J, Minna J (1985) Human L-myc (MYCL) protooncogene is on chromosome 1p32. (8th International Workshop on Human Gene Mapping) Cytogenet Cell Genet 40:694.
Naylor SL, Bishop DT (1989) Report of the committee on the genetic consitution of chromosome 3. (10th International Workshop on Human Gene Mapping) Cytogenet Cell Genet 51:106–120.
Pizon V, Chardin P, Lerosey I, Olofsson B, Tavitian A (1988) Human cDNAs RAP1 and RAP2 homologous to the Drosophila gene Dras3 encode proteins closely related to RAS in the “effector” region. Oncogene 3:201–204.
Ropers HH, Craig IW (1989) Report of the committee on the genetic constitution of chromosomes 12 and 13. (10th International Workshop on Human Gene Mapping) Cytogenet Cell Genet 51:259–279.
Rousseau-Merck M-F, Bernheim A, Chardin P, Miglierina R, Tavitian A, Berger R (1988) The RAS-retated RAL gene maps to chromosome 7p15–22. Hum Genet 79:132–136.
Rousseau-Merck MF, Zahraoui A, Bernheim A, Touchot N, Miglierina R, Tavitian A, Berger R (1989) Chromosome mapping of the human RAS-related rab3A gene to 19p13.2. Genomics 5:694–698.
Rousseau-Merck MF, Pizon V, Tavitian A, Berger R (1990) Chromosome mapping of the human RAS-related RAP1A, RAP1B and RAP2 genes to chromosomes 1p12→, 12q14 and 13q34, respectively. Cytogenet Cell Genet 53:2–4.
Segev N, Mulholland J, Botstein D (1988) The yeast GTP-binding YTP1 protein and a mammalian counterpart are associated with the secretion machinery. Cell 52:915–924.
Tokino T, Satoh H, Yoshida MC, Ochiya T, Matsubara K (1988) Regional localization of the LCA oncogene to human chromosome region 2q14→q21. Cytogenet Cell Genet 48:63–64.
Trent JM, Kaneko Y, Mitelman F (1989) Report of the committee on structural chromosome changes in neoplasia. (10th International Workshop on Human Gene Mapping) Cytogenet Cell Genet 51:533–562.
Webb AC, Collins KL, Auron PE, Eddy RL, Nakai H, Byers M, Shows TB (1985) The gene for interleukin 1 (IL1) is on human chromosome 2 at q13q21. (8th International Workshop on Human Gene Mapping) Cytogenet Cell Genet 40:774.
Yunis JJ, Sawyer JR, Ball DW (1978) Characterization of banding patterns of metaphase-prophase G-banded chromosomes and their use in gene mapping. Cytogenet Cell Genet 22:679–683.
Zahraoui A, Nicolas T, Chardin P, Tavitian A (1989) The human Rab genes encode a family of GTP-binding proteins related to yeast YPT1 and SEC4 products involved in secretion. J Biol Chem 264:12394–12401.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rousseau-Merck, MF., Zahraoui, A., Touchot, N. et al. Chromosome assignment of four RAS-related RAB genes. Hum Genet 86, 350–354 (1991). https://doi.org/10.1007/BF00201831
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00201831