Abstract
The functional intronless gene coding for a calmodulin-like protein (CLP) has been localized to human chromosome 10p13-ter. Chromosomal assignment was performed by Southern blot analysis of DNA from human-rodent somatic cell hybrids and amplification of a CLP gene-specific 1090-bp DNA fragment by the polymerase chain reaction (PCR) on DNA from human-hamster cell hybrids. Chromosomal sublocalization was carried out by in situ hybridization of human chromosome metaphase spreads. The CLP gene is the first member of the human calmodulin/calmodulin-like gene family to be chromosomally sublocalized. Its presence near the telomeric end of the short arm of chromosome 10 may be of significance with respect to its highly (epithelial) cell-type restricted expression in vivo and strong downregulation upon malignant transformation. The generation of a human CLP gene-specific sequence tag site specified by the two primers used for PCR should prove useful for future linkage studies.
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References
Feinberg AP, Vogelstein B (1983) A technique for labeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13
Heim R, Hug M, Iwata T, Strehler EE, Carafoli E (1992) Microdiversity of human-plasma-membrane calcium-pump isoform 2 generated by alternative RNA splicing in the N-terminal coding region. Eur J Biochem 205:333–340
Henderseon ER, Larson DD (1991) Telomeres — what's new at the end? Curr Opin Genet Dev 1:538–543
Klee CB, Vanaman TC (1982) Calmodulin. Adv Prot Chem 35:213–321
Koller M, Strehler EE (1988) Characterization of an intronless human calmodulin-like pseudogene. FEBS Lett 239:121–128
Koller M, Strehler EE (1992) Functional analysis of the promoters of the human CaMIII calmodulin gene and of the intronless gene coding for calmodulin-like protein. Biochim Biophys Acta (in press)
Koller M, Schnyder B, Strehler EE (1990) Structural organization of the human CaMIII calmodulin gene. Biochim Biophys Acta 1087:180–189
Koller M, Baumer A, Strehler EE (1991) Characterization of two novel human retropseudogenes related to the calmodulin-encoding gene, CaMII. Gene 97:245–251
Kunze NL, Yang GC, Jiang ZY, Hameister H, Adolph S, Wiedorn K-H, Richter A, Knippers R (1989) Localization of the active type I DNA topoisomerase gene on human chromosome 20q11. 2–13.1, and two pseudogenes on chromosomes 1q23–24 and 22q11. 2–13.1. Hum Genet 84:6–10
McPherson JD, Hickie RA, Wasmuth JJ, Meyskens FL, Perham RN, Strehler EE, Graham MT (1991) Chromosomal localization of multiple genes encoding calmodulin. Cytogenet Cell Genet 58:1951
Nojima H, Kishi K, Sokabe H (1987) Multiple calmodulin mRNA species are derived from two distinct genes. Mol Cell Biol 7:1873–1880
Nojima H, Sokabe H (1986) Structure of rat calmodulin processed genes with implications for a mRNA-mediated process of insertion. J Mol Biol 190:391–400
Nojima H (1989) Structural organization of multiple rat calmodulin genes. J Mol Biol 208:269–282
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
SenGupta B, Detera-Wadleigh SD, McBride OW, Friedberg F (1989) A calmodulin pseudogene on human chromosome 17. Nucleic Acids Res 17:1868
Simpson NE, Cann HM (1991) Report of the committee on the genetic constitution of chromosome 10. Cytogenet Cell Genet 58:428–458
Stampfer MR, Yaswen P (1992) Factors influencing growth and differentiation of normal and transformed human mammary epithelial cells in culture. In: Milo GE, Casto BC, Shuler CF (eds) Transformation of human epithelial cells: molecular and oncogenetic mechanisms. CRC Press, Boca Raton, pp 117–140
Stein JP, Munjaal RP, Lagacé L, Lai EC, O'Malley BW, Means AR (1983) Tissue-specific expression of a chicken calmodulin pseudogene lacking intervening sequences. Proc Natl Acad Sci USA 80:6485–6489
Yaswen P, Smoll A, Peehl DM, Trask DK, Sager R, Stampfer MR (1990) Down-regulation of a calmodulin-related gene during transformation of human mammary epithelial cells. Proc Natl Acad Sci USA 87:7360–7364
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Berchtold, M.W., Koller, M., Egli, R. et al. Localization of the intronless gene coding for calmodulin-like protein CLP to human chromosome 10p13-ter. Hum Genet 90, 496–500 (1993). https://doi.org/10.1007/BF00217447
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DOI: https://doi.org/10.1007/BF00217447