Abstract
Nine KOX zinc finger genes were localized on four human chromosomes by in situ hybridization of cDNA probes to metaphase chromosomes. KOX1 (ZNF10), KOX11 (ZNF18), and KOX12 (ZNF19) were mapped to chromosome bands 12q24.33, 17p13-p12, and 16q22-q23, respectively. Six other KOX genes were localized on chromosome 19: KOX6 (ZNF14) and KOX13 (ZNF20) to 19p13.3-p13.2, KOX5 (ZNF13) and KOX22 (ZNF27) to 19q13.2-qter, and KOX24 (ZNF28) and KOX28 (ZNF30) to 19q13.4. Pulsed field gel electrophoresis experiments showed that the pairs of KOX genes found on the chromosome bands 12q24.33, 16q22-q23, 19p13.3-p13.2, or 19q13.3-qter lie within 200–300 kb DNA fragments. This suggests the existence of KOX gene clusters on these chromosomal bands.
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Aubry M, Marineau C, Zhang FR, Zahed L, Figlewicz D, Delattre O, Thomas G, Jong P de, Julien JP, Rouleau GA (1992) Cloning of six new genes with zinc fingers motifs mapping to short and long arms of human acrocentric chromosomes 22 (p and q11.2). Genomics 13:641–648
Bellefroid EJ, Lecocq PJ, Benhida Q, Poncelet DA, Belayew A, Martial JA (1989) The human genome contains hundreds of genes encoding zinc finger protein of the Krüppel type. DNA 8:377–387
Bellefroid EJ, Marine J-C, Ried T, Lecocq PJ, Riviere M, Amemiya C, Poncelet DA, Colie PG, Jong P de, Szpirer C, Ward DC, Martial JA (1993) Clustered organization of homologous KRAB zinc-finger genes with enhanced expression in human T lymphoid cells. EMBO J 12:1363–1374
Bray P, Thiesen HJ (1990) Putting the finger on the DNA. New Biol 2:325–327
Bray P, Lichter P, Thiesen HJ, Ward D, Dawid IB (1991) Characterization and mapping of human genes encoding zinc finger proteins. Proc Natl Acad Sci USA 88:9563–9567
Cannizzaro LA, Aronson MM, Thiesen HJ (1993) Human zinc finger gene ZNF 23 (KOX 16) maps to a zinc finger gene cluster on chromosome 16q22 and ZNF32 (KOX 30) to chromosome region 10q23-q24. Hum Genet 91:383–385
Chowdhury K, Deutsch U, Gruss P (1987) A multiple family encoding several “finger” structures is present and differentially active in mammalian genomes. Cell 48:771–778
Feinberg AP, Vogelstein B (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13
Ginsberg A, King BO, Roeder RG (1984) Xenopus 5 S gene transcription factor, TF III A: characterization of a cDNA clone and measurement of RNA levels throughout development. Cell 39: 479–489
Harper ME, Saunders GF (1981) Localization of single copy DNA sequences on G-based human chromosomes by in situ hybridization. Chromosoma 83:431–439
Hoovers JMN, Mannens M, John R, Bliek J, Heyningen V van, Porteous DJ, Leschot NJ, Westerveld A, Little PFR (1992) High-resolution localization of 69 potential human zinc finger protein genes: a number are clustered. Genomics 12:254–263
Huebner K, Druck T, Croce CM, Thiesen HJ (1991) Twenty seven non overlapping zinc finger cDNAs from human T cells map to nine different chromosomes with apparent clustering. Am J Hum Genet 48:726–740
Lichter P, Bray P, Ried T, Dawid I, Ward D (1992) Clustering of C2-H2. Zinc finger motif sequences within telomeric and fragile site regions of human chromosomes. Genomics 13:999–1007
Lovering R, Trowsdale J (1991) A gene encoding 22 highly related zinc fingers is expressed in lymphoid cell lines. Nucleic Acids Res 19:2921–2928
McGinnis W, Krumlauf R (1992) Homeobox genes and axial patterning. Cell 68:283–302
Miller J, McLachlan AD, Klug A (1985) Repetitive zinc binding domains in the protein transcription factor III A from Xenopus oocytes. EMBO J 4:1604–1614
Panutti A, Lanfrancone L, Pascussi A, La Mantia G, Pelicci PG, Nania L (1988) Isolation of cDNA encoding finger proteins and measurements of the corresponding mRNA levels during myeloid terminal differentiation. Nucleic Acids Res 16:4227–4237
Pellegrino GR, Berg JM (1991) Identification and characterization of “zinc finger” domains by the polymerase chain reaction. Proc Natl Acad Sci USA 88:671–675
Rosenberg UB, Schroeder C, Priess A, Kielin A, Cote S, Riede J, Jackle H (1986) Structural homology of the product of the Drosophila Krüppel gene with Xenopus transcription factor III A. Nature 319:336–339
Rousseau-Merck MF, Zahraoui A, Touchot N, Tavitian A, Berger R (1991a) Chromosome assignment of four RAS-related RAB genes. Hum Genet 86:350–354
Rousseau-Merck MF, Huebner K, Berger R, Thiesen HJ (1991b) Chromosomal localization of two human zinc finger protein genes, ZNF24 (KOX 17) and ZNF29 (KOX26), to 18q12 and 17p13-12, respectively. Genomics 9:154–161
Rousseau-Merck MF, Tunnacliffe A, Berger R, Ponder BA, Thiesen HJ (1992) A cluster of expressed zinc finger protein genes in the peri centromeric region of human chromosome 10. Genomics 13:845–848
Saleh M, Selleri L, Little PFR, Evans GA (1992) Isolation and expression of linked zinc finger gene clusters on human chromosome 11q. Genomics 14:970–978
Seite P, Huebner K, Rousseau-Merck MF, Berger R, Thiesen HJ (1991) Two human genes encoding zinc finger proteins genes, ZNF12 (KOX3) and ZNF26 (KOX20), map to chromosomes 7p22-p21 and 12q24.33, respectively. Hum Genet 86:585–590
Thiessen HJ (1990) Multiple genes encoding zinc finger domains re expressed in human T cells. New Biol 2:363–374
Tunnacliffe A, Liu L, Moore JK, Leversha MA, Jackson MS, Papi L, Ferguson-Smith MA, Thiesen HJ, Ponder BAJ (1993) Duplicated KOX zinc finger gene clusters flank the centromere of human chromosome 10: evidence for a pericentric inversion during primate evolution. Nucleic Acids Res 21:1409–1417
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Rousseau-Merck, MF., Hillion, J., Jonveaux, P. et al. Chromosomal localization of 9 KOX zinc finger genes: physical linkages suggest clustering of KOX genes on chromosomes 12, 16, and 19. Hum Genet 92, 583–587 (1993). https://doi.org/10.1007/BF00420943
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DOI: https://doi.org/10.1007/BF00420943