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Ordering markers in the region of the ataxia-telangiectasia gene (11q22-q23) by fluorescence in situ hybridization (FISH) to interphase nuclei

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Abstract

Fluorescence in situ hybridization (FISH) to interphase nuclei was performed to order probes corresponding to bands 11q22-q23 where the ataxia-telangiectasia (AT) gene(s) have been located. Cosmid probes and one phage probe previously localized to this chromosome 11 region by FISH to metaphase chromosomes, were hybridized to interphase nuclei of the somatic cell hybrid J1a, which contains chromosome 11 as the only human chromosome. Two-color FISH was used with a centromeric reference probe marker. The following order was obtained: cen-D11S385 (CJ52.75)-CJ52.3-D11S384 (CJ52.193) CJ52.114-D11S424 (CJ52.77)-D11S132-NCAM-D11S351 (CJ52.208)-tel. The validity of using the centromeric probe was illustrated by showing that a probe corresponding to 11p13 hybridized more closely to the centromere than a probe corresponding to 11q22-q23, and by using cosmids hybridized three by three.

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Authors and Affiliations

  1. Unité INSERM U 301 and SDI No 16954 I CNRS, 27 Rue Juliette Dodu, F-75010, Paris, France

    Dorra Cherif, Hera Der-Sarkissian & Roland Berger

  2. CEPH, 27 Rue Juliette Dodu, F-75010, Paris, France

    Hera Der-Sarkissian

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  1. Dorra Cherif
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  2. Hera Der-Sarkissian
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  3. Roland Berger
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Cherif, D., Der-Sarkissian, H. & Berger, R. Ordering markers in the region of the ataxia-telangiectasia gene (11q22-q23) by fluorescence in situ hybridization (FISH) to interphase nuclei. Hum Genet 93, 1–6 (1994). https://doi.org/10.1007/BF00218903

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  • DOI: https://doi.org/10.1007/BF00218903

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