Skip to main content
SpringerLink
Log in

Detection by the polymerase chain reaction of two polymorphisms in exon 14 of the human inter-α-trypsin inhibitor heavy chain H1 gene

  • DNA Variants
  • Published:
Human Genetics Aims and scope Submit manuscript

Abstract

Two polymorphisms were detected within exon 14 of the inter-alpha-trypsin inhibitor heavy chain H1 (ITIH1) gene. The polymorphisms are detected by digesting the same 202-bp polymerase reaction product with the PstI and HphI restriction endonucleases. These gene polymorphisms lead to the change of two amino acids in the mature protein. The polymorphisms can be used for the analysis of 3p21 deletion in human carcinomas as well as to develop a better understanding of the protein polymorphism already described.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Balduyck M, Mizon J (1991) Inter-alpha-trypsin inhibitor and its derivatives. Ann Biol Clin 49:273–281

    Google Scholar 

  • Chen L, Mao STJ, Larsen WJ (1992) Identification of a factor in fetal bovine serum that stabilizes the cumulus extracellular matrix. A role for a member of the inter alpha-trypsin inhibitor family. J Biol Chem 267:12380–12386

    Google Scholar 

  • Diarra-Mehrpour M, Bourguignon J, Sesboüé R, Mattéi MG, Passage E, Salier JP, Martin JP (1989) Human plasma inter-alphatrypsin inhibitor is encoded by four genes on three chromosomes. Eur J Biochem 179:147–154

    Google Scholar 

  • Diarra-Mehrpour M, Bourguignon J, Bost F, Sesboüé R, Muschio F, Sarafan N, Martin JP (1992) Human inter-alpha-trypsin inhibitor: full-length cDNA sequence of the heavy chain H1. Biochim Biophys Acta 1132:114–118

    Google Scholar 

  • Killary M, Wolf M, Giambernadi T, Naylor S (1992) Definition of a tumor suppressor locus within human chromosome 3p21-p22. Proc Natl Acad Sci USA 89:10877–10881

    Google Scholar 

  • Vogt U, Cleve H (1990) A “new” genetic polymorphism of a human serum protein: inter-alpha-trypsin-inhibitor. Hum Genet 84:151–154

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut National de la Santé et de la Recherche Médicale (INSERM) Unité 295 and ERPUR (Etudes et Recherches en Pneumologie de l'Université de Rouen), UER de Médecine-Pharmacie de Rouen, Avenue de l'Université, F-76800, Saint-Etienne du Rouvray, France

    L. Thiberville, J. Bourguignon, M. Diarra-Mehrpour, G. Nouvet & J. P. Martin

  2. Service du Pr Kaplan, Biochimie Génétique, 123, Bd du Port-Royal, F-75014, Paris, France

    C. Beldjord

Authors
  1. L. Thiberville
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Bourguignon
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. Beldjord
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. Diarra-Mehrpour
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. G. Nouvet
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. J. P. Martin
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Thiberville, L., Bourguignon, J., Beldjord, C. et al. Detection by the polymerase chain reaction of two polymorphisms in exon 14 of the human inter-α-trypsin inhibitor heavy chain H1 gene. Hum Genet 93, 91–92 (1994). https://doi.org/10.1007/BF00218925

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00218925

Keywords

Search

Navigation