Summary
The ultrastructure of layers I and II of the motor cerebral cortex of rat brain has been studied at birth, 4, 7 and 14 days postnatal and in the adult.
Compared with the adult, neonatal rat motor cortex exhibited a large extracellular space which decreases with increasing age. At all stages studied the neurons were seen to contain the organelles usually found in adult neurons. Growth cones were present in decreasing numbers up to 14 days old.
Synapses were detectable at birth and there was an obvious increase in their number throughout the postnatal development. At the earliest stages studied there was a lack of specialization characteristic of the adult. Many synapses were either avesicular or relatively so and lacked the high degree of modification of adult pre- and postsynaptic membranes. By 7 days after birth many synapses existed which in all morphological respects resembled those of the adult, and by 14 days, the majority were of the adult type.
These findings, particularly with reference to the postnatal development of synapses, have been discussed in relation to the known electrophysiological findings.
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References
Aghajanian, G. K., Bloom, F. E.: The formation of synaptic junctions in developing rat brain—a quantitative electron microscopic study. Brain Res. 6, 716–727 (1967).
Armstrong-James, M. A.: Histological and neurophysiological studies on the postnatal development of the rat cerebral cortex. Ph. D. Thesis, University of Bristol (1965).
—, Johnson, F. R.: Quantitative studies of postnatal changes in synapses in rat superficial motor cortex. Z. Zellf. 110, 559–568 (1970).
-, Williams, T. D.: Postnatal development of the direct cortical response in the rat. J. Physiol. (Lond.) 168, 19P (1963).
- - Differences in the direct cortical response to unifocal stimuli during the post-natal development of the rat cerebral cortex. J. Physiol. (Lond.) 170, 15P (1964).
Bodian, D.: Development of fine structure of spinal cord in monkey fetuses. I. The motoneuron neuropil at the time of onset of reflex activity. Bull. Johns Hopk. Hosp. 119, 10–45 (1966).
—: Development of fine structure of spinal cord in monkey fetuses. II. Pre-reflex period to period of long intersegmental reflexes. J. comp. Neurol. 133, 113–166 (1968).
—: An electron microscopic characterisation of classes of synaptic vesicles by means of controlled fixation. J. Cell Biol. 44, 115–124 (1970).
Bondareff, W., Pysh, J. J.: Distribution of the extracellular space during postnatal maturation of rat cerebral cortex. Anat. Rec. 160, 773–780 (1968).
Cajal, Ramon. Y.: A quelle époque apparaissant les expansions des cellules nerveuses de la moëlle épinière du poulet? Anat. Anz. 5, 609–613, 681–689 (1890).
Caley, D. W.: Light and electron microscopic study of the developing rat cerebral cortex. Anat. Rec. 154, 325–326 (1966).
—, Maxwell, D. S.: An electron microscopic study of neurons during postnatal development of the rat cerebral cortex. J. comp. Neurol. 133, 17–44 (1968).
Cerro, M. O. del, Snider, R. S.: Studies on the developing cerebellum. Ultrastructure of the growth cones. J. comp. Neurol. 133, 341–362 (1968).
Colonnier, M.: Synaptic patterns on different cell types in the different laminae of the cat visual cortex. An electron microscope study. Brain Res. 9, 268–287 (1968).
Deza, L., Eidelberg, E.: Development of cortical electrical activity in the rat. Exp. Neurol. 17, 425–438 (1967).
Fawcett, D. W.: Surface specializations of absorbing cells. J. Histochem. Cytochem. 13, 75–91 (1965).
Flexner, L. B., Tyler, D. B., Gallant, L. J.: Biochemical and physiological differentiation during morphogenesis. X. Onset of electrical activity in developing cerebral cortex of foetal guinea pig. J. Neurophysiol. 13, 427–430 (1950).
Gray, E. G.: Axo-somatic and axo-dendritic synapses of the cerebral cortex. An electron microscope study. J. Anat. (Lond.) 93, 420–433 (1959).
—, Guillery, R. W.: Synaptic morphology in the normal and degenerating nervous system. Int. Rev. Cytol. 19, 111–182 (1966).
Harreveld, A. van, Khattab, F. I.: Perfusion fixation with glutaraldehyde and post-fixation with osmium tetroxide for electron microscopy. J. Cell Sci. 3, 579–594 (1968).
—: Changes in extracellular space of the mouse cerebral cortex during hydroxyadipaldehyde fixation and osmium tetroxide post-fixation. J. Cell Sci. 4, 437–453 (1969).
—, Malhotra, S. K.: Extracellular space in the cerebral cortex of the mouse. J. Anat. (Lond.) 101, 197–207 (1967).
Holtzman, E., Peterson, E. R.: Uptake of protein by mammalian neurons. J. Cell Biol. 40, 863–869 (1969).
Hughes, A. F., Egar, M., Turner, T.: Degeneration of nerve fibres within the embryonic spinal cord. Nature (Lond.) 221, 579–581 (1969).
Huttenlocher, P. R.: Development of cortical neuronal activity in the neonatal cat. Exp. Neurol. 17, 247–262 (1967).
Huttenlocher, P. R., Rawson, M. D.: Neuronal activity and ATPase in immature cerebral cortex. Exp. Neurol. 22, 118–129 (1968).
Larramendi, L. H. M., Fickenscher, L., Lemkey-Johnston, N.: Synaptic vesicles of inhibitory and excitatory terminals in the cerebellum. Science 156, 967–969 (1967).
Palay, S. L.: Synapses in the central nervous system. J. biophys. biochem. Cytol. (Suppl.) 2, 193–206 (1956).
Peters, A., Kaiserman-Abramof, I. R.: The small pyramidal neuron of the rat cerebral cortex. The synapses upon dendritic spines. Z. Zell. 100, 487–506 (1969).
Purpura, D. P.: Analysis of axo-dendritic synaptic organizations in immature cerebral cortex. Ann. N. Y. Acad. Sci. 94, 604–654 (1961).
—, Carmichael, M. W., Housepian, E. M.: Physiological and anatomical studies on the development of superficial axo-dendritic synaptic pathways in neocortex. Exp. Neurol. 2, 324–347 (1960).
—, Shofer, R. J., Scarff, T.: Properties of synaptic activities and spike potentials of neurons in immature neocortex. J. Neurophysiol. 28, 925–942 (1965).
Reynolds, E. S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963).
Uchizono, K.: Characteristics of excitatory and inhibitory synapses in the central nervous system of the cat. Nature (Lond.) 207, 642–643 (1965).
Vernadakis, A., Woodbury, D. M.: Cellular and extracellular spaces in developing rat brain. Arch. Neurol. (Lond.) 12, 284–293 (1965).
Voeller, K., Pappas, G. D., Purpura, D. P.: Electron microscopical study of the development of cat superficial neocortex. Exp. Neurol. 7, 107–130 (1963).
Walberg, F.: Elongated vesicles in terminal boutons of the central nervous system, a result of aldehyde fixation. Acta anat. (Basel) 65, 224–235 (1966).
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The authors wish to express their thanks to Mr. R. Birchenough and Mr. J. Manston for their technical assistance.
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Johnson, R., Armstrong-James, M. Morphology of superficial postnatal cerebral cortex with special reference to synapses. Z. Zellforsch. 110, 540–558 (1970). https://doi.org/10.1007/BF00330103
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DOI: https://doi.org/10.1007/BF00330103