Skip to main content
Log in

Scanning electron microscope observation of the dog mesenteric lymph node

  • Published:
Zeitschrift für Zellforschung und Mikroskopische Anatomie Aims and scope Submit manuscript

Summary

The perfused large mesenteric lymph node of the dog was observed under the scanning electron microscope.

The lymph sinus contains reticulum cells which mostly are two-dimensionally formed stellate plates oriented in a uniform direction. Large round macrophages are loosely fixed by the reticulum cell processes. No intermediate type between both cells has been observed. Macrophages having a few long tentacle-like projections are densely covered by clubbed cytoplasmic processes. Smaller round cells, probably plasma cells and lymphocytes also remained in the sinus.

The pulp of the node is built up by reticulum cells, much smaller than those in the sinus, and by densely packed round cells including a few macrophages.

The trabeculae and the reticulum of the nodal parenchyme form a continuous structure.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Anderson, T. F.: Techniques for the preservation of three-dimensional structure in preparing specimens for the electron microscopy. Trans. N. Y. Acad. Sci., Ser. II, 13, 130–134 (1951).

    Google Scholar 

  • Bloom, W., Fawcett, D. W.: A textbook of histology, 9th ed. Philadelphia-London-Toronto: Saunders 1968.

    Google Scholar 

  • Clark, S. L., Jr.: The reticulum of lymph nodes in mice studied with the electron microscope. Amer. J. Anat. 110, 217–258 (1962).

    Google Scholar 

  • Drinker, C. K., Wislocki, G. B., Field, M. E.: The structure of the sinuses in the lymph nodes. Anat. Rec. 56, 261–265 (1933).

    Google Scholar 

  • Han, S. S.: The ultrastructure of the mesenteric lymph node of the rat. Amer. J. Anat. 109, 183–225 (1961).

    Google Scholar 

  • Ishii, T.: Über den lichtmikroskopischen Feinbau der Retikulumfasern des Lymphknotens (ein Beitrag zur Frage von Natur und Aufbau der Retikulumfasern). Proc. Jap. Soc. Reticulo-endothelial System 6, 24–37 (1966).

    Google Scholar 

  • Maximow, A. A., Bloom, W.: A text-book of histology, 1st ed. 1930, 2nd ed. 1935. Philadelphia-London: Saunders 1935.

    Google Scholar 

  • Meyer, A. W.: The hemolymph glands of the sheep. Anat. Rec. 2, 62–64 (1908).

    Google Scholar 

  • Miyoshi, M., Fujita, T.: Stereo-fine structure of the splenic red pulp. A combined scanning and transmission electron microscope study on dog and rat spleen. Arch. histol. jap. 33, 225–246 (1971).

    Google Scholar 

  • Miyoshi, M., Fujita, T., Tokunaga, J.: The red pulp of the rabbit spleen studied under the scanning electron microscope. Arch. histol. jap. 32, 286–306 (1970).

    Google Scholar 

  • Moe, R. E.: Electron microscopic appearance of the parenchyma of lymph nodes. Amer. J. Anat. 114, 341–369 (1964).

    Google Scholar 

  • Mori, Y., Lennert, K.: Electron microscopic atlas of lymph node cytology and pathology. Berlin-Heidelberg-New York: Springer 1969.

    Google Scholar 

  • Nopajaroonsri, C., Luk, S. C., Simon, G. T.: Ultrastructure of the normal lymph node. Amer. J. Path. 65, 1–24 (1971).

    Google Scholar 

  • Sakaguchi, H.: Animal tissues under the scanning electron microscope. In: Modelling in life (ed. J. Ohta et al., in Japanese), p. 27–78. Tokyo: Kodansha 1971.

    Google Scholar 

  • Sorenson, G. D.: An electron microscopic study of popliteal lymph nodes from rabbits. Amer. J. Anat. 107, 73–96 (1960).

    Google Scholar 

  • Tanaka, K.: A simple type of apparatus for critical point drying method. J. Electronmicroscopy (Tokyo) 21, 55–56 (1972).

    Google Scholar 

  • Turner, D. R.: The vascular tree of the heamal node in the rat. J. Anat. (Lond.) 104, 481–493 (1969).

    Google Scholar 

  • Watanabe, S.: Lymph node [in Japanese]. Saibo (Tokyo) 3, Suppl. 32–36 (1971).

Download references

Author information

Authors and Affiliations

Authors

Additional information

Cordial thanks are expressed to Dr. Toshihiro Ishii, Professor of Anatomy of the Tohoku University Medical School, for his valuable advice. Thanks are also due to the kind cooperation of Mr. Akira Kubotsu of the Central Research Laboratories, Kuraray Co. Ltd.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fujita, T., Miyoshi, M. & Murakami, T. Scanning electron microscope observation of the dog mesenteric lymph node. Z.Zellforsch 133, 147–162 (1972). https://doi.org/10.1007/BF00307138

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00307138

Key words

Navigation