Summary
The brain of young domestic chicks was investigated using a Timm sulfide silver method. Serial Vibratome sections were analyzed under the light microscope, and the localization of zinc-positive structures in selected areas was determined at the ultrastructural level. Both strong and differential staining was visible in the avian telencephalon whereas most subtelencephalic structures showed a pale reaction. The highest staining intensity was found in the nonprimary sensory regions of the telencephalon such as the hyperstriatum dorsale, hyperstriatum ventrale, hippocampus, palaeostriatum augmentatum, lobus parolfactorius and caudal parts of neostriatum. There was an overall gradient of staining intensity in neostriatal areas from rostral to caudal with the heaviest zinc deposits in the caudal neostriatum. Primary sensory projection areas, such as the ectostriatum (visual), hyperstriatum intercalatum superius (visual), nucleus basalis (beak representation), the input layer L2 of the auditory field L and the somatosensory area rostral to field L were selectively left unstained. Fiber tracts throughout the brain were free of zinc deposits except for glial cells. In electron micrographs of stained regions, silver grains were localized in some presynaptic boutons of asymmetric synapses (Gray type I), within the cytoplasm of neuronal somata and sporadically in the nucleus. The possible involvement of zinc in synaptic transmission and other processes is discussed.
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Abbreviations
- Ac :
-
Nucleus accumbens
- Ad :
-
Archistriatum dorsale
- Ai :
-
Archistriatum intermedium
- Am :
-
Archistriatum mediale
- Ap :
-
Archistriatum posterior
- APH :
-
Area parahippocampalis
- BAS :
-
Nucleus basalis
- BO :
-
Bulbus olfactorius
- Cb :
-
Cerebellum;
- CbI :
-
Nucleus cerebellaris internus
- CbM :
-
Nucleus cerebellaris intermedius
- CDL :
-
Area corticoidea dorsolateralis
- CPi :
-
Cortex piriformis
- CT :
-
Commissura tectalis
- DMP :
-
Nucleus dorsomedialis posterior thalami
- E :
-
Ectostriatum
- H :
-
Hyperstriatum
- HA :
-
Hyperstriatum accessorium
- HD :
-
Hyperstriatum dorsale
- HIS :
-
Hyperstriatum intercalatum superius
- Hp :
-
Hippocampus
- HV :
-
Hyperstriatum ventrale
- ICo :
-
Nucleus intercollicularis
- Ipc :
-
Nucleus isthmi, pars parvocellularis
- L :
-
Lingula
- L 1, 2, 3 :
-
Field L
- La :
-
Nucleus laminaris
- LFM :
-
Lamina frontalis suprema
- LFS :
-
Lamina frontalis superior
- LH :
-
Lamina hyperstriatica
- LMD :
-
Lamina medullaris dorsalis
- LNH :
-
Rostrolateral neostriatum/Hyperstriatum ventrale
- LPO :
-
Lobus parolfactorius
- M :
-
Medulla
- MLd :
-
Nucleus mesencephalicus lateralis, pars dorsalis
- MNH :
-
Rostromedial neostriatum/Hyperstriatum ventrale
- N :
-
Neostriatum
- NC :
-
Neostriatum caudale
- NEB :
-
Nucleus of ectostriatal belt
- NHA :
-
Nucleus of HA
- PA :
-
Palaeostriatum augmentatum
- Pap :
-
Nucleus papillioformis
- PL :
-
Nucleus pontis lateralis
- PP :
-
Palaeostriatum primitivum
- RP :
-
Nucleus reticularis pontis caudalis
- Rt :
-
Nucleus rotundus
- S :
-
Nucleus septalis
- SS :
-
Somatosensory area
- TeO :
-
Tectum opticum
- Tn :
-
Nucleus taeniae
- TPO :
-
Area temporoparieto-occipitalis
- V :
-
Ventricle
- Va :
-
Vallecula
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Faber, H., Braun, K., Zuschratter, W. et al. System-specific distribution of zinc in the chick brain. Cell Tissue Res. 258, 247–257 (1989). https://doi.org/10.1007/BF00239445
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DOI: https://doi.org/10.1007/BF00239445