Summary
With the method of Rohr et al. (1971) the outer zona fasciculata of Sprague-Dawley-rats was compared ultramorphometrically in 5 groups of 4 animals each: males, females during diestrus, females during estrus, male and female castrates (castration on 4th day of life).
Adrenal cortical cells of the outer fasciculata in females are hypertrophic in comparison with those of normal males: females during diestrus show in comparison with males larger individual cells with increased SER, increased volume of the individual mitochondrium and total volume of mitochondria as well as an increased lipoid content together with a more pronounced dispersion of liposomes. The same function-specific structures show a further increase in females during estrus. Castration of females on the 4th day of life leads to a reduction of the function-specific morphometric parameters below the values of the diestrus animals. Male castrates show adaption of the values of their function-specific structures towards those of females during diestrus. Following castration on the 4th day of life, female animals thus show somewhat lower morphometric parameters in the outer zona fasciculata than male castrates.
Zusammenfassung
Ultramorphometrisch wurde nach dem Verfahren von Rohr et al. (1971) die äußere Zona fasciculata von Sprague-Dawley-Ratten bei 5 Gruppen zu je 4 Tieren verglichen: Männchen, Weibchen im Diöstrus, Weibchen im Östrus, männliche und weibliche Kastraten (Kastration am 4. Lebenstag).
Die Nebennierenrindenzellen der äu\eren Fasciculata sind bei Weibchen gegenüber denen normaler Männchen hypertroph: Weibchen im Diöstrus zeigen gegenüber Männchen größere Einzelzellen mit vermehrtem glattem endoplasmatischem Reticulum, erhöhten Einzel- und Gesamtvolumina der Mitochondrien sowie einem höheren Lipoidgehalt bei stärkerer Dispersion der Liposomen. Dieselben funktionsspezifischen Strukturen nehmen bei den Weibchen im Östrus noch weiter zu. Die Kastration der Weibchen am 4. Lebenstag führt zu einer Reduktion der funktionsspezifischen morphometrischen Parameter unter die Werte der Diöstrus-Tiere. Männliche Kastraten gleichen die Werte ihrer funktionsspezifischen Strukturen weitgehend denen der Weibchen im Diöstrus an. Nach Kastration am 4. Lebenstag zeigen weibliche Tiere somit etwas niedrigere morphonietrische Parameter in der äußeren Zona fasciculata als die männlichen Kastraten.
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References
Andersen, D. H., Kennedy, H. S.: Studies on physiology and reproduction; changes in adrenal gland of female rat associated with oestrus cycle. J. Physiol, (Lond.) 76, 247–260 (1932)
Colby, H. D., Gaskin, J. H., Kitay, J. I.: Requirement of the pituitary gland for gonadal hormone effects on hepatic corticosteroid metabolism in rats and hamsters. Endocrinology 92, 769–774 (1973)
Colby, H. D., Kitay, J. I.: Interaction of Testosterone and ACTH in the regulation of the adrenal corticosterone in the male rat. Endocrinology 91, 1247–1252 (1972)
Coyne, M. D., Kitay, J. I.: Effect of ovariectomy on pituitary secretion of ACTH. Endocrinology 85, 1097–1107 (1969)
Coyne, M. D., Kitay, J. I.: Effect of orchiectomy on pituitary secretion of ACTH. Endocrinology 89, 1024–1028 (1971)
Critchlow, V., Liebelt, R. A., Bar-Sela, M., Mountcastle, W., Lipscomb, H. S.: Sex difference in resting pituitary-adrenal function in the rat. Amer. J. Physiol. 205, 807–815 (1963)
Dean, F. D., Cole, P. M., Chester, J. I.: Relative rates of corticosterone secretion in intact and gonadectomized male and female rats. J. Endocr. 18, iii-iv (1959)
Dhom, G., Seebach, H. B. v., Stephan, G.: Der Geschlechtsdimorphismus der Nebenniere der Ratte, lichtmikroskopische und histochemische Untersuchungen. Z. Zellforsch. 116, 119–135 (1971)
Glenister, D. W., Yates, F. E.: Sex difference in the rate of disappearance of corticosterone4-C14 from plasma of intact rats: Further evidence for the influence of hepatic A-4-steroid hydrogenase activity of adrenal cortical function. Endocrinology 68, 747–758 (1961)
Kitay, J. I.: Sex differences in adrenal cortical secretion in the rat. Endocrinology 68, 818–824 (1961)
Kitay, J. I.: Pituitary-adrenal function in the rat after gonadectomy and gonadal hormone replacement. Endocrinology 73, 253–260 (1963)
Kitay, J. I., Coyne, M. D., Swygert, N. H., Gaines, K. E.: Effects of gonadal hormones and ACTH on the nature and rates of secretion of adrenocortical steroids by the rat. Endocrinology 89, 565–570 (1971)
Kraus, M., Křeček, J., Popp, M.: The developmet of corticosterone production by the adrenal gland in normally and prematurely weaned rats. Physiol. bohemoslov. 16, 120–127 (1967)
Mäusle, E.: Geschlechtsunterschiede in der Ultrastruktur der Nebennierenrinde der Ratte. Z. Zellforsch. 116, 136–150 (1971)
Mäusle, E., Fröhlke, M.: Geschlechtsabhängige Größenunterschiede der Mitochondrien in der Zona fasciculata der Nebennierenrinde der Ratte. Experientia (Basel) 27, 700 (1971)
Nussdorfer, G. G.: Effects of corticosteroid hormone on the smooth endoplasmic reticulum of rat adrenocortical cells. Z. Zellforsch. 106, 143–154 (1970a)
Nussdorfer, G. G., Mazzocchi, G.: Correlated morphometric and autoradiographic studies of the effect of corticosterone on adrenocortical cells of intact and hypophysectomized ACTH-treated rats. Z. Zellforsch. 111, 90–105 (1970b)
Plattner, H.: Quantitative Struktur-Funktions-Relation an Biomembranen. Progr. Histochem. Cytochem. 5, No. 3 (1973)
Rohr, H. P., Eichensberger, P.: In prep. 1974
Rohr, H. P., Riede, U. N., Sandoz, P.: Quantitative Morphologie edit. Experimentelle Abteilung d. Institutes f. Pathologie der Universität Basel (1971)
Schneider, G., French, A., Bullock, L. P., Wayne, B. C.: Absence of androgen stimulation of hepatic steroid-sulfatase in the female rat. Endocrinology 89, 308–319 (1971)
Schriefers, H., Ghraf, R., Hoff, H. G., Ockenfels, H.: Einfluß von Alter und Geschlecht auf die Entwicklung und Differenzierung der Aktivitätsmuster von Enzymen des Steroidhormon-Stoffwechsels in der Leber von Ratten zweier verschiedener Tierstämme. Hoppe Seylers Z. physiol. Chem. 352, 1363–1371 (1971)
Schriefers, H., Hoff, H. G., Ghraf, R., Ockenfels, H.: Geschlechtsund altersabhängige Entwicklung der Aktivitätsmuster von Enzymen des Steroidhormon-Stoffwechsels in der Rattenleber nach neonatalem Eingriff in die sexuelle Differenzierung. Acta endocr. (Kbh.) 69, 789–800 (1972)
Seebach, H. B. v., Dhom, G., Kubatsch, B.: Growth and development of the adrenals in postnatally castrated rats. Acta endocr. (Kbh.) Suppl. 155 (1971) 155
Sekyjama, S., Nagasami, Y.: A study in the correlation between function and ultrastructure in the rat adrenal cortex. Acta path. jap. 22, 77–98 (1972)
Skelton, F. R., Bernardis, L. L.: Effects of age, sex, hypophysectomy and gonadectomy on plasma corticosterone levels and adrenal weights following the administration of ACTH and stress. Experientia (Basel) 22, 551 (1966)
Weibel, E. R.: Stereological principles for morphometry in electron-microscopic cytology. Int. Rev. Cytol. 26, 235–299 (1969)
Weibel, E. R., Gomez, D. M.: J. appl. Physiol. 17, 343 (1962). Zit. nach Weibel, E. R.: Int. Rev. Cytol. 26, 235–299 (1969)
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We want to thank Prof. Dr. H. P. Rohr, Department of Pathology, University of Basel, for his aid with the statistical evaluation of our results and Mrs. B. Kubatsch and Mrs. S. Sigrist, Department of Pathology, University of the Saarland, for their excellent technical assistance. The electron microscope was donated by Stiftung Volkswagenwerk.
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Mäusle, E., Scherrer, H. Ultramorphometric studies regarding the effect of sex-hormones on the adrenal cortex of the rat. Cell Tissue Res. 149, 113–120 (1974). https://doi.org/10.1007/BF00209054
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DOI: https://doi.org/10.1007/BF00209054