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Influence of castration on incorporation of exogenous peroxidase into “synaptic” vesicles of the median eminence

I. The basal part of the palisade zone in male rats

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Summary

Nerve profiles in the basal part of the palisade zone of rat median eminence have been divided into 4 categories on the basis of their vesicular content: profiles a with agranular vesicles of about 50 nm, b with similar agranular vesicles and also with granular vesicles of mainly 70–140 nm, c with granular vesicles alone and d without vesicles. After intravenous injection of exogenous peroxidase, castrated male rats showed, in comparison to sham-operated controls, a 3–5 fold increase in the percentage of nerve profiles of category a which contained peroxidase loaded vesicles of about 50 nm. In contrast, no significant change was observed for nerve profiles of other categories. It is concluded that exogenous peroxidase may be used to demonstrate changes in the activity of tuberoinfundibular nerve terminals at the ultrastructural level.

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Authors and Affiliations

  1. Department of Anatomy, Erasmus University, Rotterdam

    R. Stoeckart, H. G. Jansen & A. J. Kreike

Authors
  1. R. Stoeckart
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  2. H. G. Jansen
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  3. A. J. Kreike
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Additional information

The technical assistance of Mrs. R.M.Y. Hartsteen is gratefully acknowledged. We are also grateful to Miss P. C. Delfos and Mr. W. van den Oudenalder for photographic assistance, to Mrs. L. C. Boonstra for assistance in preparing the manuscript, to Mr. J. Steysiger and Dr. J. Dullaart for measuring LH and FSH and to Dr. J. H. Aafjes and Prof. J. Moll for critical evaluation of the manuscript.

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Stoeckart, R., Jansen, H.G. & Kreike, A.J. Influence of castration on incorporation of exogenous peroxidase into “synaptic” vesicles of the median eminence. Cell Tissue Res. 155, 1–11 (1974). https://doi.org/10.1007/BF00220280

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  • DOI: https://doi.org/10.1007/BF00220280

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