Summary
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1.
The development of the characteristic histochemical appearance of the slow anterior latissimus dorsi (ALD) and fast posterior latissimus dorsi (PLD) was studied in chickens during embryonic development as well as during regeneration of minced muscle.
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2.
During embryonic development the activity of the oxidative enzyme succinic dehydrogenase (SDH) is higher in the slow ALD muscle already at 16 days of incubation. At this time the fast PLD has a higher activity of the glycolytic enzyme, phosphorylase. Although the histochemical appearance of the two types of muscle is already different at 16 days, their contractile speeds are still similar. No difference in myosin ATP-ase was found in the two muscles in young embryos but in 20-day old embryos the two muscles became distinctly different when stained for this enzyme.
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3.
When PLD muscles in hatched chickens redeveloped during regeneration in place of ALD the histochemical characteristics of the regenerated muscle resembled ALD, and when ALD regenerated in place of PLD it resembled PLD.
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4.
It is concluded that the histochemical characteristics of slow and fast muscles become determined during early development, even before any difference in contractile properties can be detected and that they are determined by the nerve.
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References
Askanas, V., Hee, D., Milhorat, A.T.: Histochemistry of aneural chicken and rat muscle in tissue culture. J. Histochem. Cytochem. 20, 849–850 (1972)
Bass, A., Lusch, G., Pette, D.: Postnatal differentiation of the enzyme activity pattern of energy-supplying metabolism in slow (red) and fast (white) muscles of chicken. Eur. J. Biochem. 13, 289–292 (1970)
Chayen, J., Bitensky, L., Butcher, R.G.: Practical histochemistry. John Wiley and Sons (1973)
Dubowitz, V.: Cross-innervated mammalian muscle: histochemical, physiological and biochemical observations. J. Physiol. 193, 481–496 (1967)
Dubowitz, V., Pearse, A.G.E.: Reciprocal relationship of phosphorylase and oxidative enzymes in skeletal muscle. Nature. 185, 701–702 (1960a)
Fedde, M.R.: Electrical properties and acetylcholine sensitivity of singly and multiply innervated avian muscle fibres. J. gen. physiol. 53, 624–637 (1969)
Godlewski, H.G.: Application of ethylene-diamine-tetracetic acid (EDTA) in the histochemical method for demonstration of phosphorylase and the branching enzyme. Bull. Acad. Pol. Sci. (Sec. biol.) 8, 441–444 (1960)
Gordon, T., Vrbová, G.: The influence of innervation on the differentiation of contractile speeds of developing chick muscles. Pflügers Arch. 360, 199–218 (1975a)
Guha, S., Wegmann, R.: Etudes sur l'activité phosphorylasique. I. Une nouvelle méthode de mise en évidence de la phosphorylase. Ann. Histochem. 3, 103–114 (1959)
Guth, L., Samaha, F.J.: Procedure for the histochemical demonstration of actomyosin ATPase. Exp. Neurol. 28, 365–367 (1970)
Hanzliková, V., Schiaffino, S.: Studies on the effect of denervation in developing muscle. III. Diversification of myofibrillar structure and origin of the heterogeneity of muscle fibre types. Z. Zellforsch. 147, 75–85 (1973)
Hnik, P., Jirmanova, J., Vyklicky, L., Zelena, J.: Fast and slow muscles of the chick after nerve cross-union. J. Physiol. 193, 309–325 (1967)
Holtzer, H., Marshall, J., Finck, H.: An analysis of myogenesis by use of fluorescent antimyosin. J. Biophys. Biochem. Cytol. 3, 705–724 (1957)
Koenig, J.: Contribution à l'étude des plaques motrices des grands dorsaux antérieur et postérieur du poulet après innervation croisée. Arch. Anat. micr. Morphol. exp. 59, 403–426 (1970)
Koenig, J., Fardeau, M.: Etude histochimique des muscles grands dorsaux antérieur et postérieur du poulet et des modifications observées après dénervation et réinnervation homologue ou croisée. Arch. Anat. micr. Morphol. exp. 62, 249–267 (1973)
Melichna, J., Gutmann, E., Syrovy, I.: Developmental changes in contraction properties, ATPase activity and muscle fibre patterns of fast and slow chicken muscle. Physiol. Bohemoslav. 23, 511–520 (1974)
Nachlas, M.M., Tsou, K., DeSouza, G., Cheng, C., Seligman, A.: Cytochemical demonstration of SDH by the use of a new P-nitrophenol substitute derivative. J. Histochem. Cytochem. 5, 420–436 (1957)
Niles, N.R., Chayen, J., Cunningham, G.J., Bitensky, L.: The histochemical demonstration of adenosine triphosphatase activity in myocardium. J. Histochem. Cytochem. 12, 740–743 (1964b)
Padykula, H.A., Herman, E.: The specificity of the histochemical method for adenosine triphosphatase. J. Histochem. Cytochem. 3, 170–183 (1955b)
Stockdale, F.E., Holtzer, H.: DNA synthesis and myogenesis. Exptl. Cell Res. 24, 508–520 (1961)
Takeuchi, T., Kuriaki, H.: Histochemical detection of phosphorylase in animal tissues. J. Histochem. Cytochem. 3, 153–160 (1953)
Vyskocil, F., Vyklicky, L., Huston, R.: Quantum content at the neuromuscular junction of fast muscle after cross union with the nerve of slow muscle in the chick. Br. Res. 26, 443–445 (1971)
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We are grateful to the Medical Research Council and Muscular Dystrophy Group of Great Britain for supporting this work and to Mrs. Fay Underhill for technical help
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Gordon, T., Perry, R., Srihari, T. et al. Differentiation of slow and fast muscles in chickens. Cell Tissue Res. 180, 211–222 (1977). https://doi.org/10.1007/BF00231953
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DOI: https://doi.org/10.1007/BF00231953