Summary
The ontogenesis of cells containing polypeptide hormones (ACTH, MSH, LPH, GH and Prolactin) was investigated in the fetal rat hypophysis by immunohistochemistry using the peroxidase-antiperoxidase complex.
Corticotrophs, melanotrophs and lipotropic cells were revealed earlier in the pars distalis than in the pars intermedia. In the pars distalis, cells producing LPH were found in the morning of day 15 of gestation using anti-γ- or anti-β-LPH sera, and in the afternoon using anti-α- or β-endorphin sera. Cells containing β-MSH were observed from the afternoon of day 15. The cells stainable with the anti-α-MSH, anti-β-(17-39)ACTH and anti-β-(l-24)ACTH sera appeared on day 16. In the pars intermedia, the cells producing α-MSH, β MSH, α- and β-endorphin, γ and β-LPH were observed in the morning of day 17, while cells containing ACTH were only revealed in the afternoon of the same day of gestation. Based on the treatment of serial paraffin sections with various antisera, it was clearly shown that MSH, ACTH, and LPH occur in the same cells located in the pars distalis as in the pars intermedia.
The development of the corticotrophs, melanotrophs and lipotropic cells does not require the presence of the fetal hypothalamus or other central nervous structures. The pituitary glands of 21 day-old fetuses encephalectomized on day 16 showed as many reactive cells as those of the littermate controls.
The somatotrophs were first revealed in the pars distalis in the afternoon of day 19. The cells producing prolactin were not observed before day 21 of gestation. On some cases GH and prolactin were found together in one cell. The cytodifferentiation of GH and prolactin cells is apparently not under hypothalamic control.
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References
Abe, K., Island, D.P., Liddle, G.W., Fleischer, N., Nicholson, W.E.: Radioimmunologic evidence for α-MSH in human pituitary and tumor tissues. J. Clin. Endocrinol. Metab. 27, 46–52 (1967)
Baker, B.L., Drummond, S.T.: The cellular origins of corticotropin and melanotropin as revealed by immunochemical staining. Am. J. Anat. 134, 395–409 (1972)
Baker, B.L., Jaffe, R.B.: The genesis of cell types in the adenohypophysis of the human fetus as observed with immunocytochemistry. Am. J. Anat. 143, 137–162 (1975)
Bégeot, M., Dubois, M.P., Dubois, P.M.: Localisation par immunofluorescence de l'hormone β lipotrope (β-LPH) et de la β endorphine dans l'antéhypophyse de foetus humains normaux et anencéphales. C.R. Acad. Sci. (Paris) 286, 213–215 (1978)
Birge, C.A., Peake, G.T., Mariz, I.K., Daughaday, W.H.: Radioimmunoassayable growth hormone in the rat pituitary gland: effects of age, sex and hormonal state. Endocrinology 81, 195–204 (1967)
Bloom, F., Battenberg, E., Rossier, J., Ling, N., Leppaluoto, J., Vargo, T.M., Guillemin, R.: Endorphins are located in the intermediate and anterior lobes of the pituitary gland, not in the neurohypophysis. Life Sci. 20, 43–48 (1977)
Briaud, B., Koch, B.: Etude en périfusion de l'effet d'extraits purifiés d'hypothalamus médio-basal sur la sécrétion d'ACTH anté- et posthypophysaire du rat. J. Physiol. (Paris) 72, 27B (1976)
Chatelain, A., Dubois, M.P., Dupouy, J.P.: Hypothalamus and cytodifferentiation of the foetal pituitary gland. Cell Tissue Res. 169, 335–344 (1976)
Chatelain, A., Dubois, M.P., Dupouy, J.P.: Les cellules à prolactine chez la ratte gestante, le foetus, le nouveau-né et le jeune. Ann. Biol. Anim. Biochim. Biophys. 17, 403–412 (1977)
Dessy, C., Herlant, M., Chretien, M.: Détection par immuno-fluorescence des cellules synthétisant la lipotropine. C.R.Acad. Sci. (Paris) 276, 335–338 (1973)
Dubois, M.P.: Cytologie de l'hypophyse des bovins: séparation par immunofluorescence, des cellules somatotropes et des cellules à prolactine. Identification des cellules LH dans la pars distalis et la pars intermedia. Bull. Assoc. Anat. 145, 139–146 (1969)
Dubois, M.P.: Etude de l'apparition des sécrétions hormonales dans l'hypophyse foetale des bovins: mise en évidence par immunofluorescence des cellules somatotropes et des cellules à prolactine. C.R. Acad. Sci. (Paris) 272, 433–435 (1971a)
Dubois, M.P.: Les cellules corticotropes de l'hypophyse des bovins, ovins et porcins. Ann. Biol. Anim. Biochim. Biophys. 11, 589–624 (1971b)
Dubois, M.P.: Localisation cytologique par immunofluorescence des sécrétions corticotropes, a et β mélanotropes au niveau de l'adénohypophyse des bovins, ovins et porcins. Z. Zellforsch. 125, 200–209 (1972a)
Dubois, M.P.: Nouvelles données sur la localisation au niveau de l'adénohypophyse des hormones polypeptidiques, ACTH, MSH, LPH. Lille Med. 17, 1391–1394; 1418–1420 (1972b)
Dubois, M.P.: Recherche par immunofluorescence des cellules adénohypophysaires élaborant les hormones polypeptidiques: ACTH, α-MSH, β-MSH. Bull. Ass. Anat. 57, 63–76 (1973)
Dubois, M.P.: Sur les sécrétions hormonales polypeptidiques du complexe mère-foetus au 30ème jour de la gestation dans l'espèce porcine. Bull. Soc. Zool. France (Suppl. 1978) (to be published)
Dubois, M.P., Graf, L.: Demonstration by immunofluorescence of the lipotropic hormone, LPH in bovine, ovine and porcine adenohypophysis. Horm. Metab. Res. 5, 229 (1973)
Dubois, P., Berthenod, M., Gilly, M., Dubois, M.P.: Etude des activités cortico-mélanotropes dans Fantéhypophyse d'un anencéphale et d'un nouveau-né normal. Arch. Fr. Pediatr. 32, 647–654 (1975)
Dubois, P., Vargues-Regairaz, H., Dubois, M.P.: Human foetal anterior pituitary immunofluorescent evidence for corticotropin and melanotropin activities. Z. Zellforsch. 145, 131–143 (1973)
Dupouy, J.P.: Evolution de la teneur de l'hypophyse foetale en ACTH. Etude chez le rat en fin de gestation. C.R. Acad. Sci. (Paris) 282, 211–214 (1976)
Dupouy, J.P., Dubois, M.P.: Ontogenesis of the α-MSH, β-MSH and ACTH cells in the fetal hypophysis of the rat. Correlation with the growth of the adrenals and adrenocortical activity. Cell Tissue Res. 161, 373–384 (1975)
Dussault, J.H., Walker, P., Dubois, J.D.: The development of the hypothalamo-pituitary axis in the neonatal rat: pituitary and serum prolactin concentrations. Can. J. Physiol. Pharmacol. 55, 84–99 (1977)
Ellis, S.T., Beck, J.S., Currie, A.R.: The cellular localisation of growth hormone in the human foetal adenohypophysis. J. Path. Bact. 92, 179–183 (1966)
Facer, P., Polak, J., Bloom, S.R., Sullivan, S.N.: Endorphin-like immunoreactivity in the human and rodent pituitary glands. J. Endocrinol. 75, 34–35P (1977)
Ferguson, K.A., Wallace, A.L.C.: Prolactin activity of human growth hormone. Nature 190, 632–633 (1961)
Goluboff, L.G., Ezrin, C.: Effect of pregnancy on the somatotroph and the prolactin cell of the human adenohypophysis. J. Clin. Endocrinol. Metab. 29, 1533–1538 (1969)
Guillemin, R., Vargo, T., Rossier, J., Minick, S., Ling, N., Rivier, C., Vale, W., Bloom, F.: β-endorphin and adrenocorticotropin are secreted concomitantly by the pituitary gland. Science 197, 1367–1369 (1977)
Hess, R., Barratt, D., Gelzer, J.: Immunofluorescent localization of gb-corticotropin in the rat pituitary. Experientia 24, 5784–5785 (1968)
Jost, A.: Anterior pituitary function in foetal life. In: The pituitary gland (G.W. Harris and B.T. Donovan, eds.), Vol. 2, pp. 299–323. London: Butterworths (1966)
Jost, A., Picon, L.: Hormonal control of fetal development and metabolism. Advances in metabolic disorders, Vol. 4, pp. 123–184. New York: Academic Press Inc. (1970)
Jost, A., Dupouy, J.P., Monchamp, A.: Fonction corticotrope de l'hypophyse et hypothalamus chez le foetus de rat. C.R. Acad. Sci. (Paris) 262, 147–150 (1966)
Jost, A., Dupouy, J.P., Rieutort, M.: The ontogenetic development of hypothalamohypophyseal relations. In: Progress in Brain Research (D.F. Swaab and Schade J.P., eds.), Vol. 41, pp. 209–219. Amsterdam: Elsevier Scientific Publ. Comp. (1974)
Kaplan, S.L., Grumbach, M.M., Shepard, T.H.: The ontogenesis of human fetal hormones. I. Growth hormone and insulin. J. Clin. Invest. 51, 3080–3093 (1972)
Kraicer, J., Gosbee, J.L., Bencosme, S.A.: Pars intermedia and pars distalis: two sites of ACTH production in the rat hypophysis. Neuroendocrinol. 11, 156–176 (1973)
Labella, F., Queen, G., Senyshyn, J.: Lipotropin: localization by radioimmunoassay of endorphin precursor in pituitary and brain. Biochem. Biophys. Res. Commun. 75, 350–357 (1977)
Lazarus, L.H., Ling, N., Guillemin, R.: β-lipotropin as a prohormone for the morphinomimetic peptides endorphins and enkephalins. Proc. Natl. Acad. Sci. U.S.A. 73, 2156–2159 (1976)
Li, C.H., Barnafi, L., Chretien, M., Chung, D.: Isolation and amino-acid sequence of β-LPH from sheep pituitary glands. Nature 208, 1093–1094 (1965)
Mains, R.E., Eipper, B.A., Ling, N.: Common precursor to corticotropins and endorphins. Proc. Natl. Acad. Sci. U.S.A. 74, 3014–3018 (1977)
Moriarty, C.M., Moriarty, G.C.: Bioactive and immunoactive ACTH in the rat pituitary: influence of stress and adrenalectomy. Endocrinol. 96, 1419–1425 (1975)
Moriarty, G.C.: Adenohypophysis: ultrastructural cytochemistry. A review. J. Histochem. Cytochem. 21, 855–894 (1973)
Moriarty, G.C., Halmi, N.S.: Electron microscopic study of the adrenocorticotropin producing cell with the use of unlabelled antibody and the soluble peroxidase-antiperoxidase complex. J. Histochem. Cytochem. 20, 590–603 (1972)
Moriarty, G.C., Halmi, N.S., Moriarty, C.M.: The effect of stress on the cytology and immunocytochemistry of pars intermedia cells in the rat pituitary. Endocrinol. 96, 1426–1436 (1975)
Nakane, P.K.: Simultaneous localization of multiple tissue antigens using the peroxydase-labelled antibody method: a study on pituitary glands of rat. J. Histochem. Cytochem. 16, 557–560 (1968)
Nakane, P.K.: Classifications of anterior pituitary cell types with immunoenzyme histochemistry. J. Histochem. Cytochem. 18, 9–20 (1970)
Nemeskery, A., Nemeth, A., Sétáló, G., Vigh, S., Halasz, B.: Cell differentiation of the fetal rat anterior pituitary in vitro. Cell Tissue Res. 170, 263–273 (1976)
Osamura, R.Y.: Functional prenatal development of anencephalic and normal pituitary glands — in human and experimental animals studied by peroxidase — labeled antibody method. Acta Pathol. Jpn. 27, 495–509 (1977)
Parker, M.L., Jarret, L., Schalch, D.S., Kipnis, D.M.: Rat growth hormone: immunofluorescent and radioimmunologic studies. Endocrinol. 76, 928–932 (1965)
Pelletier, G., Leclerc, R., Labrie, F., Cote, J., Chretien, M., Lis, M.: Immunohistochemical localization of β-lipotropic hormone in the pituitary gland. Endocrinol. 100, 770–776 (1977)
Phifer, R.F., Orth, D.N., Spicer, S.S.: Specific demonstration of the human hypophyseal adrenocortico-melanotropic (ACTH/MSH) cell. J. Clin. Endocrinol. Metab. 39, 684–695 (1974)
Phifer, R.F., Spicer, S.S.: Immunohistologic and immunopathologic demonstration of adrenocorticotropic hormone in the pars intermedia of the adenohypophysis. Lab. Invest. 23, 543–550 (1970)
Queen, G., Pinsky, C., Labella, F.: Subcellular localization of endorphine activity in bovine pituitary and brain. Biochem. Biophys. Res. Commun. 72, 1021–1027 (1976)
Rieutort, M.: Dosage radioimmunologique de l'hormone somatotrope de rat à l'aide d'une nouvelle technique de séparation. C.R. Acad. Sci. (Paris) 274, 3589–3592 (1972)
Rieutort, M., Jost, A.: Growth hormone in encephalectomized rat fetuses, with comments on the effects of anesthetics. Endocrinol. 98, 1123–1129 (1976)
Roberts, J.L., Herbert, E.: Characterization of a common precursor to corticotropin and β-lipotropin: cell-free synthesis of the precursor and identification of corticotropin peptides in the molecule. Proc. Natl. Acad. Sci. U.S.A. 74, 4826–4830 (1977)
Schleiffer, R., Mialhe, C.: Diminution du taux d'ACTH du lobe neurointermédiaire (LNI) chez le rat Brattleboro après surrénalectomie (S). J. Physiol. (Paris) 72, 28B (1976)
Scott, A.P., Lowry, P.J., Bennett, H.P.J., McMartin, C., Ratcliffe, J.G.: Purification and characterization of porcine corticotrophin-like intermediate lobe peptide. J. Endocrinol. 61, 369–380 (1974a)
Scott, A.P., Lowry, P.J., Ratcliffe, J.G., Rees. L.H., Landon, J.: Corticotropin like peptides in the rat pituitary. J. Endocrinol. 61, 355–367 (1974b)
Sétáló, G., Nakane, P.K.: Studies on the functional differentiation of cells in fetal anterior pituitary glands of rats with peroxydase labelled antibody method. Anat. Rec. 172, 403–404 (1972)
Sétáló, G., Nakane, P.K.: Functional differentiation of the fetal anterior pituitary cells in the rat. Endocrinol. Exp. 10, 155–166 (1976)
Stefan, Y., Dubois, M.P.: Localisation par immunofluorescence des hormones corticotropes et mélanotropes dans l'hypophyse du rongeur Ellobius lutescens. Z. Zellforsch. 133, 353–356 (1972)
Sternberger, L.A., Hardy, P.H., Cuculis, J.J., Meyer, H.G.: The unlabeled antibody enzyme method of immunochemistry. J. Histochem. Cytochem. 18, 315–333 (1970)
Stokes, H., Boda, J.M.: Immunofluorescent localization of growth hormone and prolactin in the adenohypophysis of fetal sheep. Endocrinol. 83, 1362–1366 (1968)
Stratmann, I.E., Ezrin, C., Sellers, E.A.: Estrogen-induced transformation of somatotrophs into mammotrophs in the rat. Cell Tissue Res. 152, 229–238 (1974)
Strosser, M.T., Mialhe, P.: L'hormone de croissance plasmatique au cours du développement foetal et postnatal chez le rat. Gen. Comp. Endocrinol. 18, 625 (1972)
Thompson, S.A., Trimble, J.J.: Immunohistochemical localization of prolactin cells of the pars distalis in the fetal and neonatal hamster. Cell Tissue Res. 168, 161–175 (1976)
Tramu, G., Dubois, M.P.: Comparative cellular localization of corticotropin and melanotropin in Lerot adenohypophysis (Eliomys quercinus). An immunohistochemical study. Cell Tissue Res. 183, 457–469 (1977)
Usategui, R., Oliver, C., Vaudry, H., Lombardi, G., Rosemberg, I., Mourre, A.M.: Immunoreactive α MSH and ACTH levels in rat plasma and pituitary. Endocrinol. 98, 189–196 (1976)
Watanabe, Y.G., Daikoku, S.: Immunological study on the cytodifferentiation of rat anterior pituitary in vivo and in vitro. 10th Int. Cong. Anat. Tokyo 275 (1975)
Watanabe, Y.G., Daikoku, S.: Immunohistochemical study on adenohypophysial primordia in organ culture. Cell Tissue Res. 166, 407–412 (1976)
Weidner, W.: Immunohistologische Untersuchungen zur Lokalisation von STH und LTH in Hypophysenvorderlappen verschiedener Spezies. Beitr. Path. Anat. 145, 168–203 (1972)
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Chatelain, A., Dupouy, J.P. & Dubois, M.P. Ontogenesis of cells producing polypeptide hormones (ACTH, MSH, LPH, GH, Prolactin) in the fetal hypophysis of the rat: Influence of the hypothalamus. Cell Tissue Res. 196, 409–427 (1979). https://doi.org/10.1007/BF00234737
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DOI: https://doi.org/10.1007/BF00234737