Summary
The distal stumps of severed medial giant axons (MGAs) and of non-giant axons (NGAs) in the CNS of the crayfish Procambarus clarkii show long-term (5–9 months) survival associated with disorientation of mitochondria and thickening of the glial sheath. However, the morphological responses of the two axonal types differ in that neither the proximal nor the distal stump of severed MGAs ever fills with mitochondria as is observed in some severed NGAs. Furthermore, the adaxonal glial layer never completely encircles portions of MGA axoplasm as occurs in many severed NGAs; in fact, ultrastructural changes in the adaxonal layer around severed MGAs are often difficult to detect. No multiple axonal profiles are ever seen within the glial sheath of the proximal or distal stumps of severed MGAs whereas these structures are easily located within severed NGAs.
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Anderson TE, Bittner GD (1980) Long term alteration of electrotonic synapses. Brain Res. 184:224–228
Atwood HL, Govind CK, Bittner GD (1973) Ultrastructure of nerve terminals and muscle fibers in denervated crayfish muscle. Z Zellforsch 146:155–166
Birse SC, Bittner GD (1976) Regeneration of giant axons in earthworms. Brain Res 113:575–581
Birse SC, Bittner GD (1980) Regeneration of transected giant axons in the earthworm CNS. (Submitted for publication)
Bittner GD (1973) Degeneration and regeneration in crustacean neuromuscular systems. Am Zool 13:379–408
Bittner GD (1977) Trophic interactions of crustacean neurons. In: Hoyle G (ed) Identified Neurons and Behavior. Plenum Press, New York, pp 507–532
Bittner GD, Ballinger M (1980) Ultrastructural studies of gap junctions between lesioned crayfish axons. Cell Tissue Res (in press)
Bittner GD, Bouton MS (1979) The mechanism of axonal regeneration in crayfish motor neurons. Neurosci Abst 5:675
Bittner GD, Johnson A (1974) Degeneration and regeneration in custacean peripheral nerves. J Comp Physiol 89:123–144
Bittner GD, Mann DW (1976) Differential survival of isolated portions of crayfish axons. Cell Tissue Res 169:301–311
Bittner GD, Ballinger ML, Larimer JL (1974) Crayfish CNS: minimal degenerative-regenerative changes after lesioning. J Exp Zool 189:13–36
Boulton PS (1969) Degeneration and regeneration in the insect central nervous system. I. Z Zellforsch 101:98–118
Clark RL (1976) Structural and functional changes in an identified cricked neuron after separation from the soma. J Comp Physiol 170:253–266
Hamori J, Horridge J (1966) The lobster optic lamina. III. Degeneration of retinula cell endings. J Cell Sci 1:271–274
Horridge GA (1968) Interneurons. WH Freeman and Co., San Francisco, pp 108–127
Hoy RR (1969) Degeneration and regeneration in abdominal flexor motor neurons in the crayfish. J Exp Zool 172:219–323
Kennedy D, Bittner GD (1974) Ultrastructural correlates of motor nerve regeneration in the crayfish. Cell Tissue Res 148:97–110
Lasek RJ, Gainer H, Barker JL (1977) Cell-to-cell transfer of glial proteins to the squid giant axon. J Cell Biol 74:501–523
Meyer MR, Bittner GD (1978a) Histological studies of trophic interactions in crayfish giant axons. Brain Res 143:195–211
Meyer MR, Bittner GD (1978b) Biochemical studies of trophic interactions in crayfish giant axons. Brain Res 143:212–232
Muller KJ, Carbonetto S (1979) The morphological and physiological properties of a regenerating synapse in the CNS of the leech. J Comp Neurol 185:485–516
Nordlander RH, Singer M (1972) Electron microscopy of severed motor fibers in the crayfish. Z Zellforsch 126:157–181
Nordlander RH, Singer M (1973) Degeneration and regeneration of crayfish sensory fibers: an ultrastructural study. J Comp Neurol 152:175–192
Singer M, Saltpeter MM (1966) The transport of 3H-l-histidine through the Schwann and myelin sheath into the axon of peripheral nerves. J Morphol 120:281–316
Spencer PS, Thomas PR (1974) Ultrastructural studies of the dying-back process II. The sequestration and removal by Schwann cells and oligodendrocytes of organelles from normal and diseased axons. J Neurocytol 3:763–783
Tung ASC, Pipa RL (1971) Fine structure of the interganglionic connectives and degenerating axons of wax moth larvae. J Ultrastruct Res 36:697–707
Wiersma CAG, Hughes GM (1961) On the functional anatomy of neuronal units in the abdominal cord of the crayfish, Procambarus clarkii (Girard). J Comp Neurol 116:209–228
Wine JJ (1973) Invertebrate central neurons: orthograde degeneration and retrograde changes after axonotomy. Exp Neurol 38:147–169
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This work was supported by NIH research grant #NS-14412 and an RCDA#00070 to GDB
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Ballinger, M.L., Bittner, G.D. Ultrastructural studies of severed medial giant and other CNS axons in crayfish. Cell Tissue Res. 208, 123–133 (1980). https://doi.org/10.1007/BF00234178
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DOI: https://doi.org/10.1007/BF00234178